Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation
Human gut microbiota harbors numerous microbial species with molecular enzymatic potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species isolation and description of their specific molecular features remain largely unexplored. In the present study, we focused...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4952 |
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| author | Ana López-Moreno Alfonso Torres-Sánchez Inmaculada Acuña Antonio Suárez Margarita Aguilera |
| author_facet | Ana López-Moreno Alfonso Torres-Sánchez Inmaculada Acuña Antonio Suárez Margarita Aguilera |
| author_sort | Ana López-Moreno |
| collection | DOAJ |
| description | Human gut microbiota harbors numerous microbial species with molecular enzymatic potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species isolation and description of their specific molecular features remain largely unexplored. In the present study, we focused on the cultivation and selection of species able to tolerate or biodegrade the endocrine disruptor bisphenol A (BPA), a xenobiotic extensively found in food plastic containers. Chemical xenobiotic addition methods for the directed isolation, culturing, Whole Genome Sequencing (WGS), phylogenomic identification, and specific gene-encoding searches have been applied to isolate microorganisms, assess their BPA metabolization potential, and describe encoded catabolic pathways. BPA-tolerant strains were isolated from 30% of infant fecal microbial culture libraries analyzed. Most isolated strains were phylogenetically related to the operational taxonomic group <i>Bacillus amyloliquefaciens</i> spp. Importantly, WGS analysis of microbial representative strain, <i>Bacillus</i> sp. AM1 identified the four complete molecular pathways involved on BPA degradation indicating its versatility and high potential to degrade BPA. Pathways for Exopolysaccharide (EPS) and Polyhydroxyalkanates (PHA) biopolymer synthesis were also identified and phenotypically confirmed by transmission electronic microscopy (TEM). These microbial biopolymers could generally contribute to capture and/or deposit xenobiotics. |
| first_indexed | 2024-03-10T11:39:38Z |
| format | Article |
| id | doaj.art-f333d2015ef94c23afa130144b15ab00 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:39:38Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f333d2015ef94c23afa130144b15ab002023-11-21T18:37:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01229495210.3390/ijms22094952Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A BiodegradationAna López-Moreno0Alfonso Torres-Sánchez1Inmaculada Acuña2Antonio Suárez3Margarita Aguilera4Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, SpainInstituto de Nutrición y Tecnología de los Alimentos, INYTA-Granada, 18100 Granada, SpainInstituto de Nutrición y Tecnología de los Alimentos, INYTA-Granada, 18100 Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, SpainHuman gut microbiota harbors numerous microbial species with molecular enzymatic potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species isolation and description of their specific molecular features remain largely unexplored. In the present study, we focused on the cultivation and selection of species able to tolerate or biodegrade the endocrine disruptor bisphenol A (BPA), a xenobiotic extensively found in food plastic containers. Chemical xenobiotic addition methods for the directed isolation, culturing, Whole Genome Sequencing (WGS), phylogenomic identification, and specific gene-encoding searches have been applied to isolate microorganisms, assess their BPA metabolization potential, and describe encoded catabolic pathways. BPA-tolerant strains were isolated from 30% of infant fecal microbial culture libraries analyzed. Most isolated strains were phylogenetically related to the operational taxonomic group <i>Bacillus amyloliquefaciens</i> spp. Importantly, WGS analysis of microbial representative strain, <i>Bacillus</i> sp. AM1 identified the four complete molecular pathways involved on BPA degradation indicating its versatility and high potential to degrade BPA. Pathways for Exopolysaccharide (EPS) and Polyhydroxyalkanates (PHA) biopolymer synthesis were also identified and phenotypically confirmed by transmission electronic microscopy (TEM). These microbial biopolymers could generally contribute to capture and/or deposit xenobiotics.https://www.mdpi.com/1422-0067/22/9/4952human microbiota<i>Bacillus</i>bisphenolsmolecular pathwaysenzymesEPS |
| spellingShingle | Ana López-Moreno Alfonso Torres-Sánchez Inmaculada Acuña Antonio Suárez Margarita Aguilera Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation International Journal of Molecular Sciences human microbiota <i>Bacillus</i> bisphenols molecular pathways enzymes EPS |
| title | Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation |
| title_full | Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation |
| title_fullStr | Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation |
| title_full_unstemmed | Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation |
| title_short | Representative <i>Bacillus</i> sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation |
| title_sort | representative i bacillus i sp am1 from gut microbiota harbor versatile molecular pathways for bisphenol a biodegradation |
| topic | human microbiota <i>Bacillus</i> bisphenols molecular pathways enzymes EPS |
| url | https://www.mdpi.com/1422-0067/22/9/4952 |
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