Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells
Abstract Cultured meat production has emerged as a breakthrough technology for the global food industry with the potential to reduce challenges associated with environmental sustainability, global public health, animal welfare, and competition for food between humans and animals. The muscle stem cel...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-44001-8 |
| _version_ | 1827590458584334336 |
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| author | Gaoxiang Zhu Dengfeng Gao Linzi Li Yixuan Yao Yingjie Wang Minglei Zhi Jinying Zhang Xinze Chen Qianqian Zhu Jie Gao Tianzhi Chen Xiaowei Zhang Tong Wang Suying Cao Aijin Ma Xianchao Feng Jianyong Han |
| author_facet | Gaoxiang Zhu Dengfeng Gao Linzi Li Yixuan Yao Yingjie Wang Minglei Zhi Jinying Zhang Xinze Chen Qianqian Zhu Jie Gao Tianzhi Chen Xiaowei Zhang Tong Wang Suying Cao Aijin Ma Xianchao Feng Jianyong Han |
| author_sort | Gaoxiang Zhu |
| collection | DOAJ |
| description | Abstract Cultured meat production has emerged as a breakthrough technology for the global food industry with the potential to reduce challenges associated with environmental sustainability, global public health, animal welfare, and competition for food between humans and animals. The muscle stem cell lines currently used for cultured meat cannot be passaged in vitro for extended periods of time. Here, we develop a directional differentiation system of porcine pre-gastrulation epiblast stem cells (pgEpiSCs) with stable cellular features and achieve serum-free myogenic differentiation of the pgEpiSCs. We show that the pgEpiSCs-derived skeletal muscle progenitor cells and skeletal muscle fibers have typical muscle cell characteristics and display skeletal muscle transcriptional features during myogenic differentiation. Importantly, we establish a three-dimensional differentiation system for shaping cultured tissue by screening plant-based edible scaffolds of non-animal origin, followed by the generation of pgEpiSCs-derived cultured meat. These advances provide a technical approach for the development of cultured meat. |
| first_indexed | 2024-03-09T01:17:08Z |
| format | Article |
| id | doaj.art-f339ad91f3e14c62be94978dca8e425d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-09T01:17:08Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f339ad91f3e14c62be94978dca8e425d2023-12-10T12:25:01ZengNature PortfolioNature Communications2041-17232023-12-0114111510.1038/s41467-023-44001-8Generation of three-dimensional meat-like tissue from stable pig epiblast stem cellsGaoxiang Zhu0Dengfeng Gao1Linzi Li2Yixuan Yao3Yingjie Wang4Minglei Zhi5Jinying Zhang6Xinze Chen7Qianqian Zhu8Jie Gao9Tianzhi Chen10Xiaowei Zhang11Tong Wang12Suying Cao13Aijin Ma14Xianchao Feng15Jianyong Han16State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityCollege of Food Science and Engineering, Northwest A&F UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityAnimal Science and Technology College, Beijing University of AgricultureSchool of Food and Health, Beijing Technology and Business UniversityCollege of Food Science and Engineering, Northwest A&F UniversityState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural UniversityAbstract Cultured meat production has emerged as a breakthrough technology for the global food industry with the potential to reduce challenges associated with environmental sustainability, global public health, animal welfare, and competition for food between humans and animals. The muscle stem cell lines currently used for cultured meat cannot be passaged in vitro for extended periods of time. Here, we develop a directional differentiation system of porcine pre-gastrulation epiblast stem cells (pgEpiSCs) with stable cellular features and achieve serum-free myogenic differentiation of the pgEpiSCs. We show that the pgEpiSCs-derived skeletal muscle progenitor cells and skeletal muscle fibers have typical muscle cell characteristics and display skeletal muscle transcriptional features during myogenic differentiation. Importantly, we establish a three-dimensional differentiation system for shaping cultured tissue by screening plant-based edible scaffolds of non-animal origin, followed by the generation of pgEpiSCs-derived cultured meat. These advances provide a technical approach for the development of cultured meat.https://doi.org/10.1038/s41467-023-44001-8 |
| spellingShingle | Gaoxiang Zhu Dengfeng Gao Linzi Li Yixuan Yao Yingjie Wang Minglei Zhi Jinying Zhang Xinze Chen Qianqian Zhu Jie Gao Tianzhi Chen Xiaowei Zhang Tong Wang Suying Cao Aijin Ma Xianchao Feng Jianyong Han Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells Nature Communications |
| title | Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells |
| title_full | Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells |
| title_fullStr | Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells |
| title_full_unstemmed | Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells |
| title_short | Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells |
| title_sort | generation of three dimensional meat like tissue from stable pig epiblast stem cells |
| url | https://doi.org/10.1038/s41467-023-44001-8 |
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