Investigation of Interleukin-17 gene polymorphism on DAP-Kinase gene promoter methylation in Patients with Breast Cancer
Background & Aim: Breast cancer is the most common malignancy in women . Studies have shown that increased in methylation of CpG islands (CpG island hyper methylation, CIHM), is one of the important mechanisms in gene down regulation. DAP-Kinase protein plays an important role in the process of...
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2016-02-01
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| Series: | Armaghane Danesh Bimonthly Journal |
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| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-191-1&slc_lang=en&sid=1 |
| Summary: | Background & Aim: Breast cancer is the most common malignancy in women . Studies have shown that increased in methylation of CpG islands (CpG island hyper methylation, CIHM), is one of the important mechanisms in gene down regulation. DAP-Kinase protein plays an important role in the process of Apoptosis. Interleukin-17 is an proinflammatory cytokine and inflammation,is one of the factors that affect on gene methylation . the purpose of this study was to evaluate the polymorphism of the IL-17 gene promoter methylation Dap-kinase and its relationship to breast cancer.
Methods: In this case - control study, A total of 40 Women with Breast cancer and 40 healthy women in Iran were examined.DNA was extracted by saluting out method and Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method and To study gene promoter methylation Dap-kinase, MSPCR method was used.data were compared in both groups by using Pearson’s chi-square and Hardy-weinberg equilibrium test.
RESULTS: Results confirm the fact that, there is a relationship between DAP-kinase gene promoter methylation and breast cancer disease So that the promoter of this gene in patients than in healthy individuals was much more methylated( p<0.05) . On the other hand there is no significant coloration between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05)
Conclusion: Due to the fact, that promoter genes methylation is one of the mechanisms of epigenetic genes silencing, it seems that DAP-kinase gene promoter methylation increases is associated with the risk of breast cancer in women. |
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| ISSN: | 1728-6506 1728-6514 |