Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
Abstract Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm the location and number of transplanted cells. This study describes in vivo imaging while assessing reporter gene exp...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-98861-5 |
| _version_ | 1818845281899249664 |
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| author | Weronika Wargocka-Matuszewska Katarzyna Fiedorowicz Anna Rugowska Karolina Bednarowicz Agnieszka Zimna Łukasz Cheda Paulina Hamankiewicz Krzysztof Kilian Michał Fiedorowicz Monika Drabik Natalia Rozwadowska Zbigniew Rogulski Maciej Kurpisz |
| author_facet | Weronika Wargocka-Matuszewska Katarzyna Fiedorowicz Anna Rugowska Karolina Bednarowicz Agnieszka Zimna Łukasz Cheda Paulina Hamankiewicz Krzysztof Kilian Michał Fiedorowicz Monika Drabik Natalia Rozwadowska Zbigniew Rogulski Maciej Kurpisz |
| author_sort | Weronika Wargocka-Matuszewska |
| collection | DOAJ |
| description | Abstract Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm the location and number of transplanted cells. This study describes in vivo imaging while assessing reporter gene expression by its binding to a radiolabelled molecule to the respective receptor expressed in target cells. Five mice underwent human skeletal muscle-derived stem/progenitor cell (huSkMDS/PC EF1-HSV-TK) intracardial transplantation after induction of myocardial infarction (MI). The metabolic parameters of control and post-infarction stem progenitor cell-implanted mice were monitored using 2-deoxy-18F-fluorodeoxyglucose ([18F]-FDG) before and after double promotor/reporter probe imaging with 9-(4-18F-fluoro-3-[hydroxymethyl]butyl)guanine ([18F]-FHBG) using positron emission tomography (PET) combined with computed tomography (CT). Standardized uptake values (SUVs) were then calculated based on set regions of interest (ROIs). Experimental animals were euthanized after magnetic resonance imaging (MRI). Molecular [18F]-FHBG imaging of myogenic stem/progenitor cells in control and post-infarction mice confirmed the survival and proliferation of transplanted cells, as shown by an increased or stable signal from the PET apparatus throughout the 5 weeks of monitoring. huSkMDS/PC EF1-HSV-TK transplantation improved cardiac metabolic ([18F]-FDG with PET) and haemodynamic (MRI) parameters. In vivo PET/CT and MRI revealed that the precise use of a promotor/reporter probe incorporated into stem/progenitor cells may improve non-invasive monitoring of targeted cellular therapy in the cardiovascular system. |
| first_indexed | 2024-12-19T05:27:10Z |
| format | Article |
| id | doaj.art-f33b98080133421aafe01e382c326a3b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:27:10Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f33b98080133421aafe01e382c326a3b2022-12-21T20:34:21ZengNature PortfolioScientific Reports2045-23222021-10-0111111210.1038/s41598-021-98861-5Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heartWeronika Wargocka-Matuszewska0Katarzyna Fiedorowicz1Anna Rugowska2Karolina Bednarowicz3Agnieszka Zimna4Łukasz Cheda5Paulina Hamankiewicz6Krzysztof Kilian7Michał Fiedorowicz8Monika Drabik9Natalia Rozwadowska10Zbigniew Rogulski11Maciej Kurpisz12Faculty of Chemistry, Biological and Chemical Research Centre, University of WarsawInstitute of Human Genetics Polish Academy of ScienceInstitute of Human Biology and Evolution, Faculty of Biology Adam, Mickiewicz UniversityInstitute of Human Genetics Polish Academy of ScienceInstitute of Human Genetics Polish Academy of ScienceFaculty of Chemistry, Biological and Chemical Research Centre, University of WarsawFaculty of Chemistry, Biological and Chemical Research Centre, University of WarsawHeavy Ion Laboratory, University of WarsawMossakowski Medical Research Centre Polish Academy of ScienceMossakowski Medical Research Centre Polish Academy of ScienceInstitute of Human Genetics Polish Academy of ScienceFaculty of Chemistry, Biological and Chemical Research Centre, University of WarsawInstitute of Human Genetics Polish Academy of ScienceAbstract Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm the location and number of transplanted cells. This study describes in vivo imaging while assessing reporter gene expression by its binding to a radiolabelled molecule to the respective receptor expressed in target cells. Five mice underwent human skeletal muscle-derived stem/progenitor cell (huSkMDS/PC EF1-HSV-TK) intracardial transplantation after induction of myocardial infarction (MI). The metabolic parameters of control and post-infarction stem progenitor cell-implanted mice were monitored using 2-deoxy-18F-fluorodeoxyglucose ([18F]-FDG) before and after double promotor/reporter probe imaging with 9-(4-18F-fluoro-3-[hydroxymethyl]butyl)guanine ([18F]-FHBG) using positron emission tomography (PET) combined with computed tomography (CT). Standardized uptake values (SUVs) were then calculated based on set regions of interest (ROIs). Experimental animals were euthanized after magnetic resonance imaging (MRI). Molecular [18F]-FHBG imaging of myogenic stem/progenitor cells in control and post-infarction mice confirmed the survival and proliferation of transplanted cells, as shown by an increased or stable signal from the PET apparatus throughout the 5 weeks of monitoring. huSkMDS/PC EF1-HSV-TK transplantation improved cardiac metabolic ([18F]-FDG with PET) and haemodynamic (MRI) parameters. In vivo PET/CT and MRI revealed that the precise use of a promotor/reporter probe incorporated into stem/progenitor cells may improve non-invasive monitoring of targeted cellular therapy in the cardiovascular system.https://doi.org/10.1038/s41598-021-98861-5 |
| spellingShingle | Weronika Wargocka-Matuszewska Katarzyna Fiedorowicz Anna Rugowska Karolina Bednarowicz Agnieszka Zimna Łukasz Cheda Paulina Hamankiewicz Krzysztof Kilian Michał Fiedorowicz Monika Drabik Natalia Rozwadowska Zbigniew Rogulski Maciej Kurpisz Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart Scientific Reports |
| title | Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart |
| title_full | Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart |
| title_fullStr | Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart |
| title_full_unstemmed | Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart |
| title_short | Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart |
| title_sort | molecular imaging of myogenic stem progenitor cells with 18f fhbg pet ct system in scid mice model of post infarction heart |
| url | https://doi.org/10.1038/s41598-021-98861-5 |
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