Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa
Objective: The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation of buffalo semen. Materials and Methods: In this experimental study, split pooled ejaculates (n=4), possessing more...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2014-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_52384335443224333224357735536427458442226-Abdolhossain%20Shahverdi-phone.pdf |
| _version_ | 1818850669007732736 |
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| author | Abdolhossain Shahverdi Abdolreza Rastegarnia Tohid Rezaei Topraggaleh |
| author_facet | Abdolhossain Shahverdi Abdolreza Rastegarnia Tohid Rezaei Topraggaleh |
| author_sort | Abdolhossain Shahverdi |
| collection | DOAJ |
| description | Objective: The aim of the present study was to investigate the effects of four equilibration
times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation
of buffalo semen.
Materials and Methods: In this experimental study, split pooled ejaculates (n=4), possessing
more than 70% visual sperm motility were divided in two aliquots and diluted in Bioxcell
® and tris-citric egg yolk (TCE) extenders. Semen was cooled to 4˚C within 2 hours,
equilibrated at 4˚C for 2, 4, 8 and 16 hours, then transferred into 0.5 ml French straws,
and frozen in a programmable cell freezer before being plunged into liquid nitrogen. Postthaw
motility characteristics, plasma membrane integrity, acrosome morphology and DNA
integrity of the buffalo sperm were studied after thawing.
Results: There were significant interactions between equilibration times and extenders
for sperm motility and membrane integrity. Post thaw sperm motility (PMOT), progressive
motile spermatozoa (PROG), plasma membrane integrity (PMI) and normal apical ridge
(NAR) measures were lower for sperm equilibrated for 2 hours in both TCE and Bioxcell®
extender compared to others equilibration times. PMOT, PMI and NAR for sperm equilibrated
for 4, 8 and 16 hours showed no significant differences in either extender, although
PROG measures were superior in Bioxcell® compared to TCE at all equilibration times
(p<0.05). Kinematic parameters such as average path velocity, curvilinear velocity and
linearity in the Bioxcell® extender were superior to those in the TCE extender studied. In
contrast to motility and viability, the DNA integrity of post thaw spermatozoa remained
unaffected by different equilibration times.
Conclusion: Equilibration time is necessary for preservation of the motility and integrity of
buffalo sperm membranes. Equilibration times of over than 2 hours resulted in the greatest
preservation of total semen parameters during cryopreservation. There were no significant
interactions between equilibration times |
| first_indexed | 2024-12-19T06:52:48Z |
| format | Article |
| id | doaj.art-f341aae00f614fc48b3178b4a5e0a54d |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-19T06:52:48Z |
| publishDate | 2014-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-f341aae00f614fc48b3178b4a5e0a54d2022-12-21T20:31:39ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142014-04-01163279288Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) SpermatozoaAbdolhossain Shahverdi0Abdolreza Rastegarnia1Tohid Rezaei Topraggaleh2Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Clinical Science, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, IranFaculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, IranObjective: The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation of buffalo semen. Materials and Methods: In this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in Bioxcell ® and tris-citric egg yolk (TCE) extenders. Semen was cooled to 4˚C within 2 hours, equilibrated at 4˚C for 2, 4, 8 and 16 hours, then transferred into 0.5 ml French straws, and frozen in a programmable cell freezer before being plunged into liquid nitrogen. Postthaw motility characteristics, plasma membrane integrity, acrosome morphology and DNA integrity of the buffalo sperm were studied after thawing. Results: There were significant interactions between equilibration times and extenders for sperm motility and membrane integrity. Post thaw sperm motility (PMOT), progressive motile spermatozoa (PROG), plasma membrane integrity (PMI) and normal apical ridge (NAR) measures were lower for sperm equilibrated for 2 hours in both TCE and Bioxcell® extender compared to others equilibration times. PMOT, PMI and NAR for sperm equilibrated for 4, 8 and 16 hours showed no significant differences in either extender, although PROG measures were superior in Bioxcell® compared to TCE at all equilibration times (p<0.05). Kinematic parameters such as average path velocity, curvilinear velocity and linearity in the Bioxcell® extender were superior to those in the TCE extender studied. In contrast to motility and viability, the DNA integrity of post thaw spermatozoa remained unaffected by different equilibration times. Conclusion: Equilibration time is necessary for preservation of the motility and integrity of buffalo sperm membranes. Equilibration times of over than 2 hours resulted in the greatest preservation of total semen parameters during cryopreservation. There were no significant interactions between equilibration timeshttp://celljournal.org/library/upload/article/af_52384335443224333224357735536427458442226-Abdolhossain%20Shahverdi-phone.pdfBuffaloSpermCryopreservationExtenderChromatinIran |
| spellingShingle | Abdolhossain Shahverdi Abdolreza Rastegarnia Tohid Rezaei Topraggaleh Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa Cell Journal Buffalo Sperm Cryopreservation Extender Chromatin Iran |
| title | Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa |
| title_full | Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa |
| title_fullStr | Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa |
| title_full_unstemmed | Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa |
| title_short | Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa |
| title_sort | effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull bubalus bubalis spermatozoa |
| topic | Buffalo Sperm Cryopreservation Extender Chromatin Iran |
| url | http://celljournal.org/library/upload/article/af_52384335443224333224357735536427458442226-Abdolhossain%20Shahverdi-phone.pdf |
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