Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics
BackgroundPolycystic ovary syndrome (PCOS) is a heterogeneous endocrinological and metabolic disorder which is the common cause of female infertility. The dysmetabolism displayed in it has not been completely ascertained. Metabonomics may shed light on understanding many small molecule endogenous me...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.982200/full |
| _version_ | 1797999441243799552 |
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| author | Shang-yue Guan Shang-yue Guan Yuan-yuan Liu Yuan-yuan Liu Yuhan Guo Yuhan Guo Xiao-xue Shen Yan Liu Yan Liu Hai-xia Jin Hai-xia Jin |
| author_facet | Shang-yue Guan Shang-yue Guan Yuan-yuan Liu Yuan-yuan Liu Yuhan Guo Yuhan Guo Xiao-xue Shen Yan Liu Yan Liu Hai-xia Jin Hai-xia Jin |
| author_sort | Shang-yue Guan |
| collection | DOAJ |
| description | BackgroundPolycystic ovary syndrome (PCOS) is a heterogeneous endocrinological and metabolic disorder which is the common cause of female infertility. The dysmetabolism displayed in it has not been completely ascertained. Metabonomics may shed light on understanding many small molecule endogenous metabolites and their associated metabolic pathways.ObjectiveTo analyze the different metabolites and related metabolic pathways in follicular fluid and embryo culture fluid of PCOS and non-PCOS groups. Finding markers predictable for clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) treatment.Population and sample60 women who underwent IVF-ET were selected, including 30 with PCOS and 30 with the fallopian tubal issues only. We collected the first tube follicular fluid (FF) of all patients at the time of oocyte pick up and the waste embryo culture medium (ECM) after D3 high-quality embryo transplant.MethodsAll samples were performed nontargeted Ultra High Performance Liquid Chromatography-Mass Spectrometry (UHPLC-QE-MS) analysis. Related metabolic pathways were screened by KEGG annotation. To search potential indicators, the logistic regression was made combined with clinical data.Mean outcome measuresPredictive performance of markers of clinical outcomes (pregnancy rate, delivery rate, live birth rate, miscarriage rate) of assisted reproductive technology (ART).ResultsComparing the PCOS group against the non-PCOS group, we found 11 significantly different metabolites in the FF and 56 in the ECM. There are a total of 11 kinds of biomarkers associated with clinical outcomes. Androsterone sulfate, Glycerophosphocholine, and Elaidic carnitine seem robust to predict the abortion rate of the PCOS group, with an AUC of 0.941, 0.933, 0.933, respectively. The glycerol phospholipid metabolic pathway is enriched in both the follicular fluid and embryo culture fluid.ConclusionsThe differential metabolites were mainly a variety of lipids. Some of them can predict clinical outcomes to a certain extent. |
| first_indexed | 2024-04-11T11:04:40Z |
| format | Article |
| id | doaj.art-f341d6da5f9040209a6b2bfdfcdb0a4d |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T11:04:40Z |
| publishDate | 2022-09-01 |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-f341d6da5f9040209a6b2bfdfcdb0a4d2022-12-22T04:28:26ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-09-011310.3389/fendo.2022.982200982200Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomicsShang-yue Guan0Shang-yue Guan1Yuan-yuan Liu2Yuan-yuan Liu3Yuhan Guo4Yuhan Guo5Xiao-xue Shen6Yan Liu7Yan Liu8Hai-xia Jin9Hai-xia Jin10Center for Reproductive Medicine, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaCenter for Reproductive Medicine, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaCenter for Reproductive Medicine, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaCenter for Reproductive Medicine, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaCenter for Reproductive Medicine, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaCenter for Reproductive Medicine, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, Zhengzhou University First Affiliated Hospital, Zhengzhou, ChinaBackgroundPolycystic ovary syndrome (PCOS) is a heterogeneous endocrinological and metabolic disorder which is the common cause of female infertility. The dysmetabolism displayed in it has not been completely ascertained. Metabonomics may shed light on understanding many small molecule endogenous metabolites and their associated metabolic pathways.ObjectiveTo analyze the different metabolites and related metabolic pathways in follicular fluid and embryo culture fluid of PCOS and non-PCOS groups. Finding markers predictable for clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) treatment.Population and sample60 women who underwent IVF-ET were selected, including 30 with PCOS and 30 with the fallopian tubal issues only. We collected the first tube follicular fluid (FF) of all patients at the time of oocyte pick up and the waste embryo culture medium (ECM) after D3 high-quality embryo transplant.MethodsAll samples were performed nontargeted Ultra High Performance Liquid Chromatography-Mass Spectrometry (UHPLC-QE-MS) analysis. Related metabolic pathways were screened by KEGG annotation. To search potential indicators, the logistic regression was made combined with clinical data.Mean outcome measuresPredictive performance of markers of clinical outcomes (pregnancy rate, delivery rate, live birth rate, miscarriage rate) of assisted reproductive technology (ART).ResultsComparing the PCOS group against the non-PCOS group, we found 11 significantly different metabolites in the FF and 56 in the ECM. There are a total of 11 kinds of biomarkers associated with clinical outcomes. Androsterone sulfate, Glycerophosphocholine, and Elaidic carnitine seem robust to predict the abortion rate of the PCOS group, with an AUC of 0.941, 0.933, 0.933, respectively. The glycerol phospholipid metabolic pathway is enriched in both the follicular fluid and embryo culture fluid.ConclusionsThe differential metabolites were mainly a variety of lipids. Some of them can predict clinical outcomes to a certain extent.https://www.frontiersin.org/articles/10.3389/fendo.2022.982200/fullassisted reproductive technology (ART)polycystic ovary syndromemetabolomicsfollicular fluidembryo culture medium |
| spellingShingle | Shang-yue Guan Shang-yue Guan Yuan-yuan Liu Yuan-yuan Liu Yuhan Guo Yuhan Guo Xiao-xue Shen Yan Liu Yan Liu Hai-xia Jin Hai-xia Jin Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics Frontiers in Endocrinology assisted reproductive technology (ART) polycystic ovary syndrome metabolomics follicular fluid embryo culture medium |
| title | Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics |
| title_full | Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics |
| title_fullStr | Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics |
| title_full_unstemmed | Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics |
| title_short | Potential biomarkers for clinical outcomes of IVF cycles in women with/without PCOS: Searching with metabolomics |
| title_sort | potential biomarkers for clinical outcomes of ivf cycles in women with without pcos searching with metabolomics |
| topic | assisted reproductive technology (ART) polycystic ovary syndrome metabolomics follicular fluid embryo culture medium |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.982200/full |
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