Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan
Objective: To study the anti-inflammatory properties of OJ. Context: Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ. Materials and methods:...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1339282 |
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| author | Sun-Young Nam Kyu-Yeob Kim Mi Hye Kim Jae-Bum Jang So-Young Rah Jin-Man Lee Hyung-Min Kim Hyun-Ja Jeong |
| author_facet | Sun-Young Nam Kyu-Yeob Kim Mi Hye Kim Jae-Bum Jang So-Young Rah Jin-Man Lee Hyung-Min Kim Hyun-Ja Jeong |
| author_sort | Sun-Young Nam |
| collection | DOAJ |
| description | Objective: To study the anti-inflammatory properties of OJ. Context: Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ. Materials and methods: Peritoneal macrophages were isolated 3–4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0.01, 0.1, and 1 mg/mL) for 1 h and stimulated with lipopolysaccharide (LPS) for different times. Nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and proinflammatory cytokine levels were determined by NO assay, Western blotting, RT-PCR and ELISA. Results: NO generation and iNOS induction were increased in the LPS-activated mouse peritoneal macrophages. However, NO generation and iNOS induction by LPS were suppressed by treatment with OJ for the first time. The IC50 value of OJ with respect to NO production was 0.09 mg/mL. OJ did not influence LPS-stimulated COX-2 induction, but did significantly decrease LPS-stimulated secretions and mRNA expressions of tumour necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Inhibition rates of TNF-α, IL-6, and IL-1β at an OJ concentration of 1 mg/mL were 77%, 88%, and 50%, respectively. OJ also suppressed the LPS-induced nuclear translocation of NF-κB. High-performance liquid chromatography showed schizandrin and gomisin A are major components of OJ. Conclusions: OJ reduces inflammatory response, and this probably explains its positive impact on the prostatitis associated inflammation. |
| first_indexed | 2024-12-23T04:35:34Z |
| format | Article |
| id | doaj.art-f341efee37b04feab29b78e805c40ced |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-23T04:35:34Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-f341efee37b04feab29b78e805c40ced2022-12-21T17:59:55ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015511856186210.1080/13880209.2017.13392821339282Anti-inflammatory effects of a traditional Korean medicine: OjayeonjonghwanSun-Young Nam0Kyu-Yeob Kim1Mi Hye Kim2Jae-Bum Jang3So-Young Rah4Jin-Man Lee5Hyung-Min Kim6Hyun-Ja Jeong7Kyung Hee UniversityKyung Hee UniversityHoseo UniversityHoseo UniversityChonbuk National UniversityHoseo UniversityKyung Hee UniversityHoseo UniversityObjective: To study the anti-inflammatory properties of OJ. Context: Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ. Materials and methods: Peritoneal macrophages were isolated 3–4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0.01, 0.1, and 1 mg/mL) for 1 h and stimulated with lipopolysaccharide (LPS) for different times. Nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and proinflammatory cytokine levels were determined by NO assay, Western blotting, RT-PCR and ELISA. Results: NO generation and iNOS induction were increased in the LPS-activated mouse peritoneal macrophages. However, NO generation and iNOS induction by LPS were suppressed by treatment with OJ for the first time. The IC50 value of OJ with respect to NO production was 0.09 mg/mL. OJ did not influence LPS-stimulated COX-2 induction, but did significantly decrease LPS-stimulated secretions and mRNA expressions of tumour necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Inhibition rates of TNF-α, IL-6, and IL-1β at an OJ concentration of 1 mg/mL were 77%, 88%, and 50%, respectively. OJ also suppressed the LPS-induced nuclear translocation of NF-κB. High-performance liquid chromatography showed schizandrin and gomisin A are major components of OJ. Conclusions: OJ reduces inflammatory response, and this probably explains its positive impact on the prostatitis associated inflammation.http://dx.doi.org/10.1080/13880209.2017.1339282mouse peritoneal macrophagesnitric oxideinflammatory cytokinenf-κb |
| spellingShingle | Sun-Young Nam Kyu-Yeob Kim Mi Hye Kim Jae-Bum Jang So-Young Rah Jin-Man Lee Hyung-Min Kim Hyun-Ja Jeong Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan Pharmaceutical Biology mouse peritoneal macrophages nitric oxide inflammatory cytokine nf-κb |
| title | Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan |
| title_full | Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan |
| title_fullStr | Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan |
| title_full_unstemmed | Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan |
| title_short | Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan |
| title_sort | anti inflammatory effects of a traditional korean medicine ojayeonjonghwan |
| topic | mouse peritoneal macrophages nitric oxide inflammatory cytokine nf-κb |
| url | http://dx.doi.org/10.1080/13880209.2017.1339282 |
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