Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) are receiving increasing interest as an approach to encapsulate natural extracts to increase the physicochemical stability of bioactives. Cannabis extract-derived cannabidiol (CBD) has potent therapeutic properties, including an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/6/2875 |
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| author | Aleksandra Zielińska Raquel da Ana Joel Fonseca Milena Szalata Karolina Wielgus Faezeh Fathi M. Beatriz P. P. Oliveira Rafał Staszewski Jacek Karczewski Eliana B. Souto |
| author_facet | Aleksandra Zielińska Raquel da Ana Joel Fonseca Milena Szalata Karolina Wielgus Faezeh Fathi M. Beatriz P. P. Oliveira Rafał Staszewski Jacek Karczewski Eliana B. Souto |
| author_sort | Aleksandra Zielińska |
| collection | DOAJ |
| description | Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) are receiving increasing interest as an approach to encapsulate natural extracts to increase the physicochemical stability of bioactives. Cannabis extract-derived cannabidiol (CBD) has potent therapeutic properties, including anti-inflammatory, antioxidant, and neuroprotective properties. In this work, physicochemical characterization was carried out after producing Compritol-based nanoparticles (cSLN or cNLC) loaded with CBD. Then, the determination of the encapsulation efficiency (EE), loading capacity (LC), particle size (Z-Ave), polydispersity index (PDI), and zeta potential were performed. Additionally, the viscoelastic profiles and differential scanning calorimetry (DSC) patterns were recorded. As a result, CBD-loaded SLN showed a mean particle size of 217.2 ± 6.5 nm, PDI of 0.273 ± 0.023, and EE of about 74%, while CBD-loaded NLC showed Z-Ave of 158.3 ± 6.6 nm, PDI of 0.325 ± 0.016, and EE of about 70%. The rheological analysis showed that the loss modulus for both lipid nanoparticle formulations was higher than the storage modulus over the applied frequency range of 10 Hz, demonstrating that they are more elastic than viscous. The crystallinity profiles of both CBD-cSLN (90.41%) and CBD-cNLC (40.18%) were determined. It may justify the obtained encapsulation parameters while corroborating the liquid-like character demonstrated in the rheological analysis. Scanning electron microscopy (SEM) study confirmed the morphology and shape of the developed nanoparticles. The work has proven that the solid nature and morphology of cSLN/cNLC strengthen these particles’ potential to modify the CBD delivery profile for several biomedical applications. |
| first_indexed | 2024-03-11T06:06:52Z |
| format | Article |
| id | doaj.art-f347f3e584214edf8ef68a5d3e18d6ac |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T06:06:52Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f347f3e584214edf8ef68a5d3e18d6ac2023-11-17T12:56:24ZengMDPI AGMolecules1420-30492023-03-01286287510.3390/molecules28062875Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid NanoparticlesAleksandra Zielińska0Raquel da Ana1Joel Fonseca2Milena Szalata3Karolina Wielgus4Faezeh Fathi5M. Beatriz P. P. Oliveira6Rafał Staszewski7Jacek Karczewski8Eliana B. Souto9Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznan, PolandDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, PortugalDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, PortugalDepartment of Biotechnology, Institute of Natural Fibres and Medicinal Plants, National Research Institute, Wojska Polskiego 71B, 60-630 Poznan, PolandDepartment of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572 Poznan, PolandREQUIMTE/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira No. 280, 4050-313 Porto, PortugalREQUIMTE/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira No. 280, 4050-313 Porto, PortugalDepartment of Hypertension Angiology and Internal Medicine, Poznan University of Medical Sciences, 61-701 Poznan, PolandDepartment of Environmental Medicine, Poznan University of Medical Sciences, 61-701 Poznan, PolandDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, PortugalSolid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) are receiving increasing interest as an approach to encapsulate natural extracts to increase the physicochemical stability of bioactives. Cannabis extract-derived cannabidiol (CBD) has potent therapeutic properties, including anti-inflammatory, antioxidant, and neuroprotective properties. In this work, physicochemical characterization was carried out after producing Compritol-based nanoparticles (cSLN or cNLC) loaded with CBD. Then, the determination of the encapsulation efficiency (EE), loading capacity (LC), particle size (Z-Ave), polydispersity index (PDI), and zeta potential were performed. Additionally, the viscoelastic profiles and differential scanning calorimetry (DSC) patterns were recorded. As a result, CBD-loaded SLN showed a mean particle size of 217.2 ± 6.5 nm, PDI of 0.273 ± 0.023, and EE of about 74%, while CBD-loaded NLC showed Z-Ave of 158.3 ± 6.6 nm, PDI of 0.325 ± 0.016, and EE of about 70%. The rheological analysis showed that the loss modulus for both lipid nanoparticle formulations was higher than the storage modulus over the applied frequency range of 10 Hz, demonstrating that they are more elastic than viscous. The crystallinity profiles of both CBD-cSLN (90.41%) and CBD-cNLC (40.18%) were determined. It may justify the obtained encapsulation parameters while corroborating the liquid-like character demonstrated in the rheological analysis. Scanning electron microscopy (SEM) study confirmed the morphology and shape of the developed nanoparticles. The work has proven that the solid nature and morphology of cSLN/cNLC strengthen these particles’ potential to modify the CBD delivery profile for several biomedical applications.https://www.mdpi.com/1420-3049/28/6/2875solid lipid nanoparticlesnanostructured lipid carrierscannabidiolviscoelastic behaviorCompritol<sup>®</sup> 888 ATOMiglyol<sup>®</sup> 812 |
| spellingShingle | Aleksandra Zielińska Raquel da Ana Joel Fonseca Milena Szalata Karolina Wielgus Faezeh Fathi M. Beatriz P. P. Oliveira Rafał Staszewski Jacek Karczewski Eliana B. Souto Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles Molecules solid lipid nanoparticles nanostructured lipid carriers cannabidiol viscoelastic behavior Compritol<sup>®</sup> 888 ATO Miglyol<sup>®</sup> 812 |
| title | Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles |
| title_full | Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles |
| title_fullStr | Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles |
| title_full_unstemmed | Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles |
| title_short | Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles |
| title_sort | phytocannabinoids chromatographic screening of cannabinoids and loading into lipid nanoparticles |
| topic | solid lipid nanoparticles nanostructured lipid carriers cannabidiol viscoelastic behavior Compritol<sup>®</sup> 888 ATO Miglyol<sup>®</sup> 812 |
| url | https://www.mdpi.com/1420-3049/28/6/2875 |
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