Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis
Cucumber is one of the most widely cultivated greenhouse vegetables, and its quality and yield are threatened by drought stress. Studies have shown that carbon dioxide concentration ([CO<sub>2</sub>]) enrichment can alleviate drought stress in cucumber seedlings; however the mechanism of...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14911 |
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| author | Yiman Li Wendong Zhang Dalong Zhang Yinjian Zheng Yaliang Xu Binbin Liu Qingming Li |
| author_facet | Yiman Li Wendong Zhang Dalong Zhang Yinjian Zheng Yaliang Xu Binbin Liu Qingming Li |
| author_sort | Yiman Li |
| collection | DOAJ |
| description | Cucumber is one of the most widely cultivated greenhouse vegetables, and its quality and yield are threatened by drought stress. Studies have shown that carbon dioxide concentration ([CO<sub>2</sub>]) enrichment can alleviate drought stress in cucumber seedlings; however the mechanism of this [CO<sub>2</sub>] enrichment effect on root drought stress is not clear. In this study, the effects of different drought stresses (simulated with 0, 5% and 10% PEG 6000, i.e., no, moderate, and severe drought stress) and [CO<sub>2</sub>] (400 μmol·mol<sup>−1</sup> and 800 ± 40 μmol·mol<sup>−1</sup>) on the cucumber seedling root proteome were analyzed using the tandem mass tag (TMT) quantitative proteomics method. The results showed that after [CO<sub>2</sub>] enrichment, 346 differentially accumulating proteins (DAPs) were found only under moderate drought stress, 27 DAPs only under severe drought stress, and 34 DAPs under both moderate and severe drought stress. [CO<sub>2</sub>] enrichment promoted energy metabolism, amino acid metabolism, and secondary metabolism, induced the expression of proteins related to root cell wall and cytoskeleton metabolism, effectively maintained the balance of protein processing and degradation, and enhanced the cell wall regulation ability. However, the extent to which [CO<sub>2</sub>] enrichment alleviated drought stress in cucumber seedling roots was limited under severe drought stress, which may be due to excessive damage to the seedlings. |
| first_indexed | 2024-03-09T17:46:01Z |
| format | Article |
| id | doaj.art-f34954bc71bb49de8716379a3ba3a1d5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:01Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f34954bc71bb49de8716379a3ba3a1d52023-11-24T11:10:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231491110.3390/ijms232314911Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical AnalysisYiman Li0Wendong Zhang1Dalong Zhang2Yinjian Zheng3Yaliang Xu4Binbin Liu5Qingming Li6College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Water Resource and Civil Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, ChinaState Key Laboratory of Crop Biology, Tai’an 271018, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, ChinaCucumber is one of the most widely cultivated greenhouse vegetables, and its quality and yield are threatened by drought stress. Studies have shown that carbon dioxide concentration ([CO<sub>2</sub>]) enrichment can alleviate drought stress in cucumber seedlings; however the mechanism of this [CO<sub>2</sub>] enrichment effect on root drought stress is not clear. In this study, the effects of different drought stresses (simulated with 0, 5% and 10% PEG 6000, i.e., no, moderate, and severe drought stress) and [CO<sub>2</sub>] (400 μmol·mol<sup>−1</sup> and 800 ± 40 μmol·mol<sup>−1</sup>) on the cucumber seedling root proteome were analyzed using the tandem mass tag (TMT) quantitative proteomics method. The results showed that after [CO<sub>2</sub>] enrichment, 346 differentially accumulating proteins (DAPs) were found only under moderate drought stress, 27 DAPs only under severe drought stress, and 34 DAPs under both moderate and severe drought stress. [CO<sub>2</sub>] enrichment promoted energy metabolism, amino acid metabolism, and secondary metabolism, induced the expression of proteins related to root cell wall and cytoskeleton metabolism, effectively maintained the balance of protein processing and degradation, and enhanced the cell wall regulation ability. However, the extent to which [CO<sub>2</sub>] enrichment alleviated drought stress in cucumber seedling roots was limited under severe drought stress, which may be due to excessive damage to the seedlings.https://www.mdpi.com/1422-0067/23/23/14911cucumber rootsCO<sub>2</sub> enrichmentdrought stressTMT-based quantitative proteomiccarbohydrate synthesisamino acid metabolism |
| spellingShingle | Yiman Li Wendong Zhang Dalong Zhang Yinjian Zheng Yaliang Xu Binbin Liu Qingming Li Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis International Journal of Molecular Sciences cucumber roots CO<sub>2</sub> enrichment drought stress TMT-based quantitative proteomic carbohydrate synthesis amino acid metabolism |
| title | Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis |
| title_full | Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis |
| title_fullStr | Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis |
| title_full_unstemmed | Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis |
| title_short | Mechanism of [CO<sub>2</sub>] Enrichment Alleviated Drought Stress in the Roots of Cucumber Seedlings Revealed via Proteomic and Biochemical Analysis |
| title_sort | mechanism of co sub 2 sub enrichment alleviated drought stress in the roots of cucumber seedlings revealed via proteomic and biochemical analysis |
| topic | cucumber roots CO<sub>2</sub> enrichment drought stress TMT-based quantitative proteomic carbohydrate synthesis amino acid metabolism |
| url | https://www.mdpi.com/1422-0067/23/23/14911 |
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