The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella
Microorganisms have developed many strategies in the process of long-term defense against external attacks, one of which is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological system. In this study, the whole genome of 300 s...
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| Format: | Article |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.790673/full |
| _version_ | 1818320306326994944 |
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| author | Yuqiao Zhou Wei Zhou Jinzhi Zhou Jinchang Yan Dingting Xu Xiner Zheng Shuaizhou Zong Ping Jiang Shiyi Tian Jianzhong Han Daofeng Qu |
| author_facet | Yuqiao Zhou Wei Zhou Jinzhi Zhou Jinchang Yan Dingting Xu Xiner Zheng Shuaizhou Zong Ping Jiang Shiyi Tian Jianzhong Han Daofeng Qu |
| author_sort | Yuqiao Zhou |
| collection | DOAJ |
| description | Microorganisms have developed many strategies in the process of long-term defense against external attacks, one of which is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological system. In this study, the whole genome of 300 strains of Klebsiella was collected, the CRISPR-Cas system in the strains was statistically analyzed, and the types and structures of CRISPR system in Klebsiella were explored, as well as the correlation between CRISPR and mobile genetic elements (MGEs). Through principal component analysis (PCA), we found that Cas gene, plasmids, integron, IS1, IS609, and enzymes of DNA metabolism were closely related to CRISPR-Cas. Compared the structural characteristics of plasmids, the DinG family helicases, Cas6, Csf2, and IS5 were observed near the CRISPR loci in plasmid, which is also confirmed by the results of PCA that they may be important factors affecting the plasmid with CRISPR. |
| first_indexed | 2024-12-13T10:22:55Z |
| format | Article |
| id | doaj.art-f34a5413c19646f2b4d350b7d82276da |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T10:22:55Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f34a5413c19646f2b4d350b7d82276da2022-12-21T23:51:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-02-011210.3389/fmicb.2021.790673790673The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in KlebsiellaYuqiao Zhou0Wei Zhou1Jinzhi Zhou2Jinchang Yan3Dingting Xu4Xiner Zheng5Shuaizhou Zong6Ping Jiang7Shiyi Tian8Jianzhong Han9Daofeng Qu10Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, ChinaZhejiang Provincial Center for Animal Disease Prevention and Control, Hangzhou, ChinaZhejiang Provincial Center for Animal Disease Prevention and Control, Hangzhou, ChinaAgricultural and Rural Bureau of Wangdian Town, Jiaxing, ChinaThe Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, ChinaThe Eighth People’s Hospital of Qingdao, Qingdao, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, ChinaMicroorganisms have developed many strategies in the process of long-term defense against external attacks, one of which is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological system. In this study, the whole genome of 300 strains of Klebsiella was collected, the CRISPR-Cas system in the strains was statistically analyzed, and the types and structures of CRISPR system in Klebsiella were explored, as well as the correlation between CRISPR and mobile genetic elements (MGEs). Through principal component analysis (PCA), we found that Cas gene, plasmids, integron, IS1, IS609, and enzymes of DNA metabolism were closely related to CRISPR-Cas. Compared the structural characteristics of plasmids, the DinG family helicases, Cas6, Csf2, and IS5 were observed near the CRISPR loci in plasmid, which is also confirmed by the results of PCA that they may be important factors affecting the plasmid with CRISPR.https://www.frontiersin.org/articles/10.3389/fmicb.2021.790673/fullKlebsiellaCRISPR-Castype IVplasmidPCA |
| spellingShingle | Yuqiao Zhou Wei Zhou Jinzhi Zhou Jinchang Yan Dingting Xu Xiner Zheng Shuaizhou Zong Ping Jiang Shiyi Tian Jianzhong Han Daofeng Qu The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella Frontiers in Microbiology Klebsiella CRISPR-Cas type IV plasmid PCA |
| title | The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella |
| title_full | The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella |
| title_fullStr | The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella |
| title_full_unstemmed | The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella |
| title_short | The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella |
| title_sort | clustered regularly interspaced short palindromic repeats associated system and its relationship with mobile genetic elements in klebsiella |
| topic | Klebsiella CRISPR-Cas type IV plasmid PCA |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.790673/full |
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