Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics
Previously, DNA microarrays analysis showed that, in co-culture with <i>Bacillus subtilis</i>, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of <i>Aspergillus niger</i> is downregulated. Based on phylogenetic and synteny analyses, we show here t...
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MDPI AG
2021-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/5/374 |
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| author | Gregory Evdokias Cameron Semper Montserrat Mora-Ochomogo Marcos Di Falco Thi Truc Minh Nguyen Alexei Savchenko Adrian Tsang Isabelle Benoit-Gelber |
| author_facet | Gregory Evdokias Cameron Semper Montserrat Mora-Ochomogo Marcos Di Falco Thi Truc Minh Nguyen Alexei Savchenko Adrian Tsang Isabelle Benoit-Gelber |
| author_sort | Gregory Evdokias |
| collection | DOAJ |
| description | Previously, DNA microarrays analysis showed that, in co-culture with <i>Bacillus subtilis</i>, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of <i>Aspergillus niger</i> is downregulated. Based on phylogenetic and synteny analyses, we show here that this gene cluster, <i>NRRL3_00036-NRRL3_00042</i>, comprises genes predicted to encode a nonribosomal peptides synthetase, a FAD-binding domain-containing protein, an uncharacterized protein, a transporter, a cytochrome P450 protein, a NAD(P)-binding domain-containing protein and a transcription factor. We overexpressed the in-cluster transcription factor gene <i>NRRL3_00042</i>. The overexpression strain, NRRL3_00042<sup>OE</sup>, displays reduced growth rate and production of a yellow pigment, which by mass spectrometric analysis corresponds to two compounds with masses of 409.1384 and 425.1331. We deleted the gene encoding the NRRL3_00036 nonribosomal peptides synthetase in the NRRL3_00042<sup>OE</sup> strain. The resulting strain reverted to the wild-type phenotype. These results suggest that the biosynthetic gene cluster anchored by the <i>NRRL3_00036</i> nonribosomal peptides synthetase gene is regulated by the in-cluster transcriptional regulator gene <i>NRRL3_00042</i>, and that it is involved in the production of two previously uncharacterized compounds. |
| first_indexed | 2024-03-10T11:33:14Z |
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| language | English |
| last_indexed | 2024-03-10T11:33:14Z |
| publishDate | 2021-05-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-f34c7f8856744974a07ff8621cc9cb312023-11-21T19:08:11ZengMDPI AGJournal of Fungi2309-608X2021-05-017537410.3390/jof7050374Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative GenomicsGregory Evdokias0Cameron Semper1Montserrat Mora-Ochomogo2Marcos Di Falco3Thi Truc Minh Nguyen4Alexei Savchenko5Adrian Tsang6Isabelle Benoit-Gelber7Centre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, CanadaDepartment of Microbiology, Immunology and Infectious Disease, University of Calgary, 3330 Hospital Drive, Calgary, AB T2N 4N1, CanadaCentre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, CanadaCentre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, CanadaCentre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, CanadaDepartment of Microbiology, Immunology and Infectious Disease, University of Calgary, 3330 Hospital Drive, Calgary, AB T2N 4N1, CanadaCentre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, CanadaCentre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, CanadaPreviously, DNA microarrays analysis showed that, in co-culture with <i>Bacillus subtilis</i>, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of <i>Aspergillus niger</i> is downregulated. Based on phylogenetic and synteny analyses, we show here that this gene cluster, <i>NRRL3_00036-NRRL3_00042</i>, comprises genes predicted to encode a nonribosomal peptides synthetase, a FAD-binding domain-containing protein, an uncharacterized protein, a transporter, a cytochrome P450 protein, a NAD(P)-binding domain-containing protein and a transcription factor. We overexpressed the in-cluster transcription factor gene <i>NRRL3_00042</i>. The overexpression strain, NRRL3_00042<sup>OE</sup>, displays reduced growth rate and production of a yellow pigment, which by mass spectrometric analysis corresponds to two compounds with masses of 409.1384 and 425.1331. We deleted the gene encoding the NRRL3_00036 nonribosomal peptides synthetase in the NRRL3_00042<sup>OE</sup> strain. The resulting strain reverted to the wild-type phenotype. These results suggest that the biosynthetic gene cluster anchored by the <i>NRRL3_00036</i> nonribosomal peptides synthetase gene is regulated by the in-cluster transcriptional regulator gene <i>NRRL3_00042</i>, and that it is involved in the production of two previously uncharacterized compounds.https://www.mdpi.com/2309-608X/7/5/374<i>Aspergillus niger</i>secondary metabolitesBGC: biosynthetic gene clusterNRPS: nonribosomal peptide synthetasecomparative genomicsCRISPR/Cas9 |
| spellingShingle | Gregory Evdokias Cameron Semper Montserrat Mora-Ochomogo Marcos Di Falco Thi Truc Minh Nguyen Alexei Savchenko Adrian Tsang Isabelle Benoit-Gelber Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics Journal of Fungi <i>Aspergillus niger</i> secondary metabolites BGC: biosynthetic gene cluster NRPS: nonribosomal peptide synthetase comparative genomics CRISPR/Cas9 |
| title | Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics |
| title_full | Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics |
| title_fullStr | Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics |
| title_full_unstemmed | Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics |
| title_short | Identification of a Novel Biosynthetic Gene Cluster in <i>Aspergillus niger</i> Using Comparative Genomics |
| title_sort | identification of a novel biosynthetic gene cluster in i aspergillus niger i using comparative genomics |
| topic | <i>Aspergillus niger</i> secondary metabolites BGC: biosynthetic gene cluster NRPS: nonribosomal peptide synthetase comparative genomics CRISPR/Cas9 |
| url | https://www.mdpi.com/2309-608X/7/5/374 |
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