Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model
Excessive liver fat causes non-alcoholic fatty liver disease (NAFLD) in laying hens, reducing egg production. Addressing NAFLD via bile-acid metabolism is gaining attention. We induced NAFLD in 7-week-old ISA female chickens with a high-cholesterol, low-choline diet (CLC) for 6 weeks. LC/MS was used...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/7/992 |
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| author | Wen-Yuan Yang Pei-En Chang Sin-Jin Li Shih-Torng Ding Yuan-Yu Lin |
| author_facet | Wen-Yuan Yang Pei-En Chang Sin-Jin Li Shih-Torng Ding Yuan-Yu Lin |
| author_sort | Wen-Yuan Yang |
| collection | DOAJ |
| description | Excessive liver fat causes non-alcoholic fatty liver disease (NAFLD) in laying hens, reducing egg production. Addressing NAFLD via bile-acid metabolism is gaining attention. We induced NAFLD in 7-week-old ISA female chickens with a high-cholesterol, low-choline diet (CLC) for 6 weeks. LC/MS was used to analyze serum and cecal bile acids, while cecal digesta DNA underwent 16S rRNA sequencing. The distribution of bile acid varied in healthy (CON) and CLC-fed chickens. CLC increased secondary bile acids (TLCA, TUDCA, THDCA, TDCA) in serum and primary bile acids (CDCA, TCDCA, isoDCA) in serum, as well as glycochenodeoxycholic acid (GCDCA) in cecal contents. CLC upregulated bile-acid synthesis enzymes (CYP7A1, CYP8B1) in the liver. Bile-acid receptor gene expression (HNF4A, FXR, LXR) was similar between groups. Microbiota abundance was richer in CON (alpha-diversity), with distinct separation (beta-diversity) between CON and CLC. The Firmicutes/Bacteroidetes ratio slightly decreased in CLC. Taxonomic analysis revealed higher <i>Bacteroides</i>, <i>Alistipes</i>, <i>Megamonas</i> in CLC but lower <i>Barnesiella</i>. CLC had more Mucispirillum, Eubacterium_coprostanoligenes_group, Shuttleworthia, and Olsenella, while CON had more Enterococcus, Ruminococcaceae_UCG_014, and Faecalibacterium. This study unveils bile-acid and microflora changes in a chicken NAFLD model, enhancing our understanding of fatty liver disease metabolism and aiding targeted interventions. |
| first_indexed | 2024-04-24T10:51:34Z |
| format | Article |
| id | doaj.art-f350660d60d1424b8b1675204121dfb8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-24T10:51:34Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f350660d60d1424b8b1675204121dfb82024-04-12T13:14:04ZengMDPI AGAnimals2076-26152024-03-0114799210.3390/ani14070992Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease ModelWen-Yuan Yang0Pei-En Chang1Sin-Jin Li2Shih-Torng Ding3Yuan-Yu Lin4Department of Animal Science and Technology, National Taiwan University, Taipei City 106, TaiwanInstitute of Biotechnology, National Taiwan University, Taipei City 106, TaiwanBachelor Program of Biotechnology and Food Nutrition, National Taiwan University, Taipei City 106, TaiwanDepartment of Animal Science and Technology, National Taiwan University, Taipei City 106, TaiwanDepartment of Animal Science and Technology, National Taiwan University, Taipei City 106, TaiwanExcessive liver fat causes non-alcoholic fatty liver disease (NAFLD) in laying hens, reducing egg production. Addressing NAFLD via bile-acid metabolism is gaining attention. We induced NAFLD in 7-week-old ISA female chickens with a high-cholesterol, low-choline diet (CLC) for 6 weeks. LC/MS was used to analyze serum and cecal bile acids, while cecal digesta DNA underwent 16S rRNA sequencing. The distribution of bile acid varied in healthy (CON) and CLC-fed chickens. CLC increased secondary bile acids (TLCA, TUDCA, THDCA, TDCA) in serum and primary bile acids (CDCA, TCDCA, isoDCA) in serum, as well as glycochenodeoxycholic acid (GCDCA) in cecal contents. CLC upregulated bile-acid synthesis enzymes (CYP7A1, CYP8B1) in the liver. Bile-acid receptor gene expression (HNF4A, FXR, LXR) was similar between groups. Microbiota abundance was richer in CON (alpha-diversity), with distinct separation (beta-diversity) between CON and CLC. The Firmicutes/Bacteroidetes ratio slightly decreased in CLC. Taxonomic analysis revealed higher <i>Bacteroides</i>, <i>Alistipes</i>, <i>Megamonas</i> in CLC but lower <i>Barnesiella</i>. CLC had more Mucispirillum, Eubacterium_coprostanoligenes_group, Shuttleworthia, and Olsenella, while CON had more Enterococcus, Ruminococcaceae_UCG_014, and Faecalibacterium. This study unveils bile-acid and microflora changes in a chicken NAFLD model, enhancing our understanding of fatty liver disease metabolism and aiding targeted interventions.https://www.mdpi.com/2076-2615/14/7/992non-alcoholic fatty liverbile acidscecal microbiotahigh-cholesterol low-choline diet |
| spellingShingle | Wen-Yuan Yang Pei-En Chang Sin-Jin Li Shih-Torng Ding Yuan-Yu Lin Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model Animals non-alcoholic fatty liver bile acids cecal microbiota high-cholesterol low-choline diet |
| title | Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model |
| title_full | Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model |
| title_fullStr | Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model |
| title_full_unstemmed | Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model |
| title_short | Exploring Bile-Acid Changes and Microflora Profiles in Chicken Fatty Liver Disease Model |
| title_sort | exploring bile acid changes and microflora profiles in chicken fatty liver disease model |
| topic | non-alcoholic fatty liver bile acids cecal microbiota high-cholesterol low-choline diet |
| url | https://www.mdpi.com/2076-2615/14/7/992 |
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