Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes
Aspartate aminotransferase was purified from urine and serum of patients with type 2 diabetes in a 2 steps procedure involving dialysis bag and sephadex G-25 gel filtration (column chromatography). The enzyme was purified 346.23 fold with 1467% yield and 3.46 fold with 142.85% yield in urine and se...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Arabic |
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Mustansiriyah University/College of Engineering
2012-12-01
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| Series: | Journal of Engineering and Sustainable Development |
| Subjects: | |
| Online Access: | https://jeasd.uomustansiriyah.edu.iq/index.php/jeasd/article/view/1256 |
| _version_ | 1797984794403930112 |
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| author | Tagreed U.Mohammd Saad S. Saleh Nadia M.Al-Mhana Azhaar F.Abd Al-Zhra |
| author_facet | Tagreed U.Mohammd Saad S. Saleh Nadia M.Al-Mhana Azhaar F.Abd Al-Zhra |
| author_sort | Tagreed U.Mohammd |
| collection | DOAJ |
| description |
Aspartate aminotransferase was purified from urine and serum of patients with type 2 diabetes in a 2 steps procedure involving dialysis bag and sephadex G-25 gel filtration (column chromatography). The enzyme was purified 346.23 fold with 1467% yield and 3.46 fold with 142.85% yield in urine and serum of patients with type 2 diabetes respectively. The purified enzyme showed single peak. The results of this study revealed that AST activity of type 2 diabetes urine and serum increased significantly (p<0.001) compared with control group.
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| first_indexed | 2024-04-11T07:08:27Z |
| format | Article |
| id | doaj.art-f350ada5bebb4478afae75af55f4dd8d |
| institution | Directory Open Access Journal |
| issn | 2520-0917 2520-0925 |
| language | Arabic |
| last_indexed | 2024-04-11T07:08:27Z |
| publishDate | 2012-12-01 |
| publisher | Mustansiriyah University/College of Engineering |
| record_format | Article |
| series | Journal of Engineering and Sustainable Development |
| spelling | doaj.art-f350ada5bebb4478afae75af55f4dd8d2022-12-22T04:38:17ZaraMustansiriyah University/College of EngineeringJournal of Engineering and Sustainable Development2520-09172520-09252012-12-01164Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 DiabetesTagreed U.Mohammd0Saad S. Saleh1Nadia M.Al-Mhana2Azhaar F.Abd Al-Zhra3Chemistry Dept., College of Education-Ibn AL- Haytham, Baghdad University, Baghdad, Iraq Chemistry Dept., College of Science for Women, University of Baghdad, Baghdad, IraqChemistry Dept., College of Education-Ibn AL- Haytham, Baghdad University, Baghdad, Iraq College of Engineering, Al-Mustansiriyah University, Baghdad, Iraq Aspartate aminotransferase was purified from urine and serum of patients with type 2 diabetes in a 2 steps procedure involving dialysis bag and sephadex G-25 gel filtration (column chromatography). The enzyme was purified 346.23 fold with 1467% yield and 3.46 fold with 142.85% yield in urine and serum of patients with type 2 diabetes respectively. The purified enzyme showed single peak. The results of this study revealed that AST activity of type 2 diabetes urine and serum increased significantly (p<0.001) compared with control group. https://jeasd.uomustansiriyah.edu.iq/index.php/jeasd/article/view/1256Aspartate aminotransferasePurificationDiabetes |
| spellingShingle | Tagreed U.Mohammd Saad S. Saleh Nadia M.Al-Mhana Azhaar F.Abd Al-Zhra Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes Journal of Engineering and Sustainable Development Aspartate aminotransferase Purification Diabetes |
| title | Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes |
| title_full | Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes |
| title_fullStr | Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes |
| title_full_unstemmed | Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes |
| title_short | Comparison Study of (Urinary & Serum) AST Activity from Patients with Type 2 Diabetes |
| title_sort | comparison study of urinary serum ast activity from patients with type 2 diabetes |
| topic | Aspartate aminotransferase Purification Diabetes |
| url | https://jeasd.uomustansiriyah.edu.iq/index.php/jeasd/article/view/1256 |
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