A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size
Summary: Skeletal muscle size is controlled by the balance between protein synthesis and protein degradation. Given the essential role of skeletal muscle in maintaining a high quality of life, understanding the mechanisms that modulate this balance are of critical importance. Previously, we demonstr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422300603X |
| _version_ | 1797848280377327616 |
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| author | Nathaniel D. Steinert Kent W. Jorgenson Kuan-Hung Lin Jake B. Hermanson Jake L. Lemens Troy A. Hornberger |
| author_facet | Nathaniel D. Steinert Kent W. Jorgenson Kuan-Hung Lin Jake B. Hermanson Jake L. Lemens Troy A. Hornberger |
| author_sort | Nathaniel D. Steinert |
| collection | DOAJ |
| description | Summary: Skeletal muscle size is controlled by the balance between protein synthesis and protein degradation. Given the essential role of skeletal muscle in maintaining a high quality of life, understanding the mechanisms that modulate this balance are of critical importance. Previously, we demonstrated that muscle-specific knockout of TRIM28 reduces muscle size and function and in the current study, we discovered that this effect is associated with an increase in protein degradation and a dramatic reduction in the expression of Mettl21c. Importantly, we also determined that overexpression of Mettl21c is sufficient to induce hypertrophy in both control and TRIM28 knockout muscles. Moreover, we developed a simple pulse-chase biorthogonal non-canonical amino acid tagging technique that enabled us to visualize the in vivo rate of protein degradation, and with this technique were able to conclude that the hypertrophic effect of Mettl21c is due, at least in part, to an inhibition of protein degradation. |
| first_indexed | 2024-04-09T18:24:58Z |
| format | Article |
| id | doaj.art-f350e5a34bf94357b778af9c6864303c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T18:24:58Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-f350e5a34bf94357b778af9c6864303c2023-04-12T04:12:09ZengElsevieriScience2589-00422023-04-01264106526A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle sizeNathaniel D. Steinert0Kent W. Jorgenson1Kuan-Hung Lin2Jake B. Hermanson3Jake L. Lemens4Troy A. Hornberger5Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USADepartment of Molecular and Cellular Pharmacology, University of Wisconsin - Madison, Madison, WI, USA; School of Medicine and Public Health, University of Wisconsin - Madison, Madison, WI, USADepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USADepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USADepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USADepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA; Corresponding authorSummary: Skeletal muscle size is controlled by the balance between protein synthesis and protein degradation. Given the essential role of skeletal muscle in maintaining a high quality of life, understanding the mechanisms that modulate this balance are of critical importance. Previously, we demonstrated that muscle-specific knockout of TRIM28 reduces muscle size and function and in the current study, we discovered that this effect is associated with an increase in protein degradation and a dramatic reduction in the expression of Mettl21c. Importantly, we also determined that overexpression of Mettl21c is sufficient to induce hypertrophy in both control and TRIM28 knockout muscles. Moreover, we developed a simple pulse-chase biorthogonal non-canonical amino acid tagging technique that enabled us to visualize the in vivo rate of protein degradation, and with this technique were able to conclude that the hypertrophic effect of Mettl21c is due, at least in part, to an inhibition of protein degradation.http://www.sciencedirect.com/science/article/pii/S258900422300603XBiochemistryCell biologyDevelopmental biology |
| spellingShingle | Nathaniel D. Steinert Kent W. Jorgenson Kuan-Hung Lin Jake B. Hermanson Jake L. Lemens Troy A. Hornberger A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size iScience Biochemistry Cell biology Developmental biology |
| title | A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size |
| title_full | A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size |
| title_fullStr | A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size |
| title_full_unstemmed | A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size |
| title_short | A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size |
| title_sort | novel method for visualizing in vivo rates of protein degradation provides insight into how trim28 regulates muscle size |
| topic | Biochemistry Cell biology Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422300603X |
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