Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species.
An analysis of the phospholipid ester-linked and the lipopolysaccharide (LPS) fatty acids and hydroxy fatty acids of six lactate-utilizing Desulfovibrio-type sulfate-reducing bacteria (SRB) has been performed using capillary gas-liquid chromatography-mass spectrometry (GLC-MS). The concentrations of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1985-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520343029 |
| _version_ | 1819055426362146816 |
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| author | A Edlund P D Nichols R Roffey D C White |
| author_facet | A Edlund P D Nichols R Roffey D C White |
| author_sort | A Edlund |
| collection | DOAJ |
| description | An analysis of the phospholipid ester-linked and the lipopolysaccharide (LPS) fatty acids and hydroxy fatty acids of six lactate-utilizing Desulfovibrio-type sulfate-reducing bacteria (SRB) has been performed using capillary gas-liquid chromatography-mass spectrometry (GLC-MS). The concentrations of normal fatty acids were essentially similar, with the possible exception of a high content of normal fatty acids in the LPS of Desulfovibrio gigas. Determination of monounsaturated acid double bond configuration was performed by GLC-MS analysis of the derivatized fatty acids. A total of nine branched chain and eight straight chain monounsaturated fatty acids was detected in the Desulfovibrio species analyzed. The major component detected in five Desulfovibrio was the 17-carbon iso-branched monoenoic acid which showed cis unsaturation [i17:1(n-7)c] seven carbons from the terminal methyl group of the fatty acid chain. D. gigas, in contrast, contained almost no unsaturated fatty acids and was greatly enriched in iso-branched 15:0. Major differences between strains were found in the phospholipid and LPS hydroxy fatty acids. These components, in addition to the i17:1(n-7)c and other characteristic branched chain unsaturated acids, can possibly be utilized as signatures of the lactate-utilizing SRB. |
| first_indexed | 2024-12-21T13:07:20Z |
| format | Article |
| id | doaj.art-f357611d015a48e4a6f7dbc236c568e4 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:07:20Z |
| publishDate | 1985-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-f357611d015a48e4a6f7dbc236c568e42022-12-21T19:02:58ZengElsevierJournal of Lipid Research0022-22751985-08-01268982988Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species.A EdlundP D NicholsR RoffeyD C WhiteAn analysis of the phospholipid ester-linked and the lipopolysaccharide (LPS) fatty acids and hydroxy fatty acids of six lactate-utilizing Desulfovibrio-type sulfate-reducing bacteria (SRB) has been performed using capillary gas-liquid chromatography-mass spectrometry (GLC-MS). The concentrations of normal fatty acids were essentially similar, with the possible exception of a high content of normal fatty acids in the LPS of Desulfovibrio gigas. Determination of monounsaturated acid double bond configuration was performed by GLC-MS analysis of the derivatized fatty acids. A total of nine branched chain and eight straight chain monounsaturated fatty acids was detected in the Desulfovibrio species analyzed. The major component detected in five Desulfovibrio was the 17-carbon iso-branched monoenoic acid which showed cis unsaturation [i17:1(n-7)c] seven carbons from the terminal methyl group of the fatty acid chain. D. gigas, in contrast, contained almost no unsaturated fatty acids and was greatly enriched in iso-branched 15:0. Major differences between strains were found in the phospholipid and LPS hydroxy fatty acids. These components, in addition to the i17:1(n-7)c and other characteristic branched chain unsaturated acids, can possibly be utilized as signatures of the lactate-utilizing SRB.http://www.sciencedirect.com/science/article/pii/S0022227520343029 |
| spellingShingle | A Edlund P D Nichols R Roffey D C White Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. Journal of Lipid Research |
| title | Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. |
| title_full | Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. |
| title_fullStr | Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. |
| title_full_unstemmed | Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. |
| title_short | Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. |
| title_sort | extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from desulfovibrio species |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520343029 |
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