Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves.
The fresh leaves of Mitragyna speciosa (Korth.) Havil. have been traditionally consumed for centuries in Southeast Asia for its healing properties. Although the alkaloids of M. speciosa have been studied since the 1920s, comparative and systematic studies of metabolite composition based on different...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0283147 |
| _version_ | 1797843014885834752 |
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| author | Rubashiny Veeramohan Arief Izzairy Zamani Kamalrul Azlan Azizan Hoe-Han Goh Wan Mohd Aizat Mohd Fauzi Abd Razak Nur Sabrina Mohd Yusof Sharif Mahsufi Mansor Syarul Nataqain Baharum Chyan Leong Ng |
| author_facet | Rubashiny Veeramohan Arief Izzairy Zamani Kamalrul Azlan Azizan Hoe-Han Goh Wan Mohd Aizat Mohd Fauzi Abd Razak Nur Sabrina Mohd Yusof Sharif Mahsufi Mansor Syarul Nataqain Baharum Chyan Leong Ng |
| author_sort | Rubashiny Veeramohan |
| collection | DOAJ |
| description | The fresh leaves of Mitragyna speciosa (Korth.) Havil. have been traditionally consumed for centuries in Southeast Asia for its healing properties. Although the alkaloids of M. speciosa have been studied since the 1920s, comparative and systematic studies of metabolite composition based on different leaf maturity levels are still lacking. This study assessed the secondary metabolite composition in two different leaf stages (young and mature) of M. speciosa, using an untargeted liquid chromatography-electrospray ionisation-time-of-flight-mass spectrometry (LC-ESI-TOF-MS) metabolite profiling. The results revealed 86 putatively annotated metabolite features (RT:m/z value) comprising 63 alkaloids, 10 flavonoids, 6 terpenoids, 3 phenylpropanoids, and 1 of each carboxylic acid, glucoside, phenol, and phenolic aldehyde. The alkaloid features were further categorised into 14 subclasses, i.e., the most abundant class of secondary metabolites identified. As per previous reports, indole alkaloids are the most abundant alkaloid subclass in M. speciosa. The result of multivariate analysis (MVA) using principal component analysis (PCA) showed a clear separation of 92.8% between the young and mature leaf samples, indicating a high variance in metabolite levels between them. Akuammidine, alstonine, tryptamine, and yohimbine were tentatively identified among the many new alkaloids reported in this study, depicting the diverse biological activities of M. speciosa. Besides delving into the knowledge of metabolite distribution in different leaf stages, these findings have extended the current alkaloid repository of M. speciosa for a better understanding of its pharmaceutical potential. |
| first_indexed | 2024-04-09T16:58:41Z |
| format | Article |
| id | doaj.art-f359b382acc7456bb74fcc8ab4fcaef7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T16:58:41Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f359b382acc7456bb74fcc8ab4fcaef72023-04-21T05:34:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01183e028314710.1371/journal.pone.0283147Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves.Rubashiny VeeramohanArief Izzairy ZamaniKamalrul Azlan AzizanHoe-Han GohWan Mohd AizatMohd Fauzi Abd RazakNur Sabrina Mohd YusofSharif Mahsufi MansorSyarul Nataqain BaharumChyan Leong NgThe fresh leaves of Mitragyna speciosa (Korth.) Havil. have been traditionally consumed for centuries in Southeast Asia for its healing properties. Although the alkaloids of M. speciosa have been studied since the 1920s, comparative and systematic studies of metabolite composition based on different leaf maturity levels are still lacking. This study assessed the secondary metabolite composition in two different leaf stages (young and mature) of M. speciosa, using an untargeted liquid chromatography-electrospray ionisation-time-of-flight-mass spectrometry (LC-ESI-TOF-MS) metabolite profiling. The results revealed 86 putatively annotated metabolite features (RT:m/z value) comprising 63 alkaloids, 10 flavonoids, 6 terpenoids, 3 phenylpropanoids, and 1 of each carboxylic acid, glucoside, phenol, and phenolic aldehyde. The alkaloid features were further categorised into 14 subclasses, i.e., the most abundant class of secondary metabolites identified. As per previous reports, indole alkaloids are the most abundant alkaloid subclass in M. speciosa. The result of multivariate analysis (MVA) using principal component analysis (PCA) showed a clear separation of 92.8% between the young and mature leaf samples, indicating a high variance in metabolite levels between them. Akuammidine, alstonine, tryptamine, and yohimbine were tentatively identified among the many new alkaloids reported in this study, depicting the diverse biological activities of M. speciosa. Besides delving into the knowledge of metabolite distribution in different leaf stages, these findings have extended the current alkaloid repository of M. speciosa for a better understanding of its pharmaceutical potential.https://doi.org/10.1371/journal.pone.0283147 |
| spellingShingle | Rubashiny Veeramohan Arief Izzairy Zamani Kamalrul Azlan Azizan Hoe-Han Goh Wan Mohd Aizat Mohd Fauzi Abd Razak Nur Sabrina Mohd Yusof Sharif Mahsufi Mansor Syarul Nataqain Baharum Chyan Leong Ng Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves. PLoS ONE |
| title | Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves. |
| title_full | Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves. |
| title_fullStr | Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves. |
| title_full_unstemmed | Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves. |
| title_short | Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves. |
| title_sort | comparative metabolomics analysis reveals alkaloid repertoires in young and mature mitragyna speciosa korth havil leaves |
| url | https://doi.org/10.1371/journal.pone.0283147 |
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