Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population
Molecular characterization of a given set of maize germplasm could be useful for understanding the use of the assembled germplasm for further improvement in a breeding program, such as analyzing genetic diversity, selecting a parental line, assigning heterotic groups, creating a core set of germplas...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1226072/full |
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| author | Alejandro Ledesma Fernando Augusto Sales Ribeiro Alison Uberti Jode Edwards Sarah Hearne Ursula Frei Thomas Lübberstedt |
| author_facet | Alejandro Ledesma Fernando Augusto Sales Ribeiro Alison Uberti Jode Edwards Sarah Hearne Ursula Frei Thomas Lübberstedt |
| author_sort | Alejandro Ledesma |
| collection | DOAJ |
| description | Molecular characterization of a given set of maize germplasm could be useful for understanding the use of the assembled germplasm for further improvement in a breeding program, such as analyzing genetic diversity, selecting a parental line, assigning heterotic groups, creating a core set of germplasm and/or performing association analysis for traits of interest. In this study, we used single nucleotide polymorphism (SNP) markers to assess the genetic variability in a set of doubled haploid (DH) lines derived from the unselected Iowa Stiff Stalk Synthetic (BSSS) maize population, denoted as C0 (BSSS(R)C0), the seventeenth cycle of reciprocal recurrent selection in BSSS (BSSS(R)C17), denoted as C17 and the cross between BSSS(R)C0 and BSSS(R)C17 denoted as C0/C17. With the aim to explore if we have potentially lost diversity from C0 to C17 derived DH lines and observe whether useful genetic variation in C0 was left behind during the selection process since C0 could be a reservoir of genetic diversity that could be untapped using DH technology. Additionally, we quantify the contribution of the BSSS progenitors in each set of DH lines. The molecular characterization analysis confirmed the apparent separation and the loss of genetic variability from C0 to C17 through the recurrent selection process. Which was observed by the degree of differentiation between the C0_DHL versus C17_DHL groups by Wright’s F-statistics (FST). Similarly for the population structure based on principal component analysis (PCA) revealed a clear separation among groups of DH lines. Some of the progenitors had a higher genetic contribution in C0 compared with C0/C17 and C17 derived DH lines. Although genetic drift can explain most of the genetic structure genome-wide, phenotypic data provide evidence that selection has altered favorable allele frequencies in the BSSS maize population through the reciprocal recurrent selection program. |
| first_indexed | 2024-03-12T20:32:47Z |
| format | Article |
| id | doaj.art-f35c5d5d859e40798c09659592d1a562 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T20:32:47Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f35c5d5d859e40798c09659592d1a5622023-08-01T19:15:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.12260721226072Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize populationAlejandro Ledesma0Fernando Augusto Sales Ribeiro1Alison Uberti2Jode Edwards3Sarah Hearne4Ursula Frei5Thomas Lübberstedt6Department of Agronomy, Iowa State University, Ames, IA, United StatesDepartment of Agronomy, Iowa State University, Ames, IA, United StatesDepartment of Agronomy, Iowa State University, Ames, IA, United StatesUSDA-ARS, Corn Insects and Crop Genetics Research Unit, Ames, IA, United StatesInternational Maize and Wheat Improvement Center (CIMMYT), El Batan, Texcoco, MexicoDepartment of Agronomy, Iowa State University, Ames, IA, United StatesDepartment of Agronomy, Iowa State University, Ames, IA, United StatesMolecular characterization of a given set of maize germplasm could be useful for understanding the use of the assembled germplasm for further improvement in a breeding program, such as analyzing genetic diversity, selecting a parental line, assigning heterotic groups, creating a core set of germplasm and/or performing association analysis for traits of interest. In this study, we used single nucleotide polymorphism (SNP) markers to assess the genetic variability in a set of doubled haploid (DH) lines derived from the unselected Iowa Stiff Stalk Synthetic (BSSS) maize population, denoted as C0 (BSSS(R)C0), the seventeenth cycle of reciprocal recurrent selection in BSSS (BSSS(R)C17), denoted as C17 and the cross between BSSS(R)C0 and BSSS(R)C17 denoted as C0/C17. With the aim to explore if we have potentially lost diversity from C0 to C17 derived DH lines and observe whether useful genetic variation in C0 was left behind during the selection process since C0 could be a reservoir of genetic diversity that could be untapped using DH technology. Additionally, we quantify the contribution of the BSSS progenitors in each set of DH lines. The molecular characterization analysis confirmed the apparent separation and the loss of genetic variability from C0 to C17 through the recurrent selection process. Which was observed by the degree of differentiation between the C0_DHL versus C17_DHL groups by Wright’s F-statistics (FST). Similarly for the population structure based on principal component analysis (PCA) revealed a clear separation among groups of DH lines. Some of the progenitors had a higher genetic contribution in C0 compared with C0/C17 and C17 derived DH lines. Although genetic drift can explain most of the genetic structure genome-wide, phenotypic data provide evidence that selection has altered favorable allele frequencies in the BSSS maize population through the reciprocal recurrent selection program.https://www.frontiersin.org/articles/10.3389/fpls.2023.1226072/fullzea mays L.diversitygenetic resourceshomozygous linesgenetic diversity |
| spellingShingle | Alejandro Ledesma Fernando Augusto Sales Ribeiro Alison Uberti Jode Edwards Sarah Hearne Ursula Frei Thomas Lübberstedt Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population Frontiers in Plant Science zea mays L. diversity genetic resources homozygous lines genetic diversity |
| title | Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population |
| title_full | Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population |
| title_fullStr | Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population |
| title_full_unstemmed | Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population |
| title_short | Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population |
| title_sort | molecular characterization of doubled haploid lines derived from different cycles of the iowa stiff stalk synthetic bsss maize population |
| topic | zea mays L. diversity genetic resources homozygous lines genetic diversity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1226072/full |
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