Proteomics of secretory and endocytic organelles in Giardia lamblia.
Giardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocy...
| Asıl Yazarlar: | , , , , |
|---|---|
| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Public Library of Science (PLoS)
2014-01-01
|
| Seri Bilgileri: | PLoS ONE |
| Online Erişim: | http://europepmc.org/articles/PMC3986054?pdf=render |
| _version_ | 1828895915016978432 |
|---|---|
| author | Petra B Wampfler Vinko Tosevski Paolo Nanni Cornelia Spycher Adrian B Hehl |
| author_facet | Petra B Wampfler Vinko Tosevski Paolo Nanni Cornelia Spycher Adrian B Hehl |
| author_sort | Petra B Wampfler |
| collection | DOAJ |
| description | Giardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocytic organelles termed peripheral vesicles (PVs), mainly on the exposed dorsal side. When trophozoites reach the G2/M restriction point in the cell cycle they can begin another round of cell division or encyst if they encounter specific environmental cues. They induce neogenesis of Golgi-like organelles, encystation-specific vesicles (ESVs), for regulated secretion of cyst wall material. PVs and ESVs are highly simplified and thus evolutionary diverged endocytic and exocytic organelle systems with key roles in proliferation and transmission to a new host, respectively. Both organelle systems physically and functionally intersect at the endoplasmic reticulum (ER) which has catabolic as well as anabolic functions. However, the unusually high degree of sequence divergence in Giardia rapidly exhausts phylogenomic strategies to identify and characterize the molecular underpinnings of these streamlined organelles. To define the first proteome of ESVs and PVs we used a novel strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data. From the limited size datasets we retrieved many hypothetical but also known organelle-specific factors. In contrast to PVs, ESVs appear to maintain a strong physical and functional link to the ER including recruitment of ribosomes to organelle membranes. Overall the data provide further evidence for the formation of a cyst extracellular matrix with minimal complexity. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000694. |
| first_indexed | 2024-12-13T14:34:33Z |
| format | Article |
| id | doaj.art-f35eb0cd2c5c45abb993c25461fadb51 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:34:33Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f35eb0cd2c5c45abb993c25461fadb512022-12-21T23:41:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9408910.1371/journal.pone.0094089Proteomics of secretory and endocytic organelles in Giardia lamblia.Petra B WampflerVinko TosevskiPaolo NanniCornelia SpycherAdrian B HehlGiardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocytic organelles termed peripheral vesicles (PVs), mainly on the exposed dorsal side. When trophozoites reach the G2/M restriction point in the cell cycle they can begin another round of cell division or encyst if they encounter specific environmental cues. They induce neogenesis of Golgi-like organelles, encystation-specific vesicles (ESVs), for regulated secretion of cyst wall material. PVs and ESVs are highly simplified and thus evolutionary diverged endocytic and exocytic organelle systems with key roles in proliferation and transmission to a new host, respectively. Both organelle systems physically and functionally intersect at the endoplasmic reticulum (ER) which has catabolic as well as anabolic functions. However, the unusually high degree of sequence divergence in Giardia rapidly exhausts phylogenomic strategies to identify and characterize the molecular underpinnings of these streamlined organelles. To define the first proteome of ESVs and PVs we used a novel strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data. From the limited size datasets we retrieved many hypothetical but also known organelle-specific factors. In contrast to PVs, ESVs appear to maintain a strong physical and functional link to the ER including recruitment of ribosomes to organelle membranes. Overall the data provide further evidence for the formation of a cyst extracellular matrix with minimal complexity. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000694.http://europepmc.org/articles/PMC3986054?pdf=render |
| spellingShingle | Petra B Wampfler Vinko Tosevski Paolo Nanni Cornelia Spycher Adrian B Hehl Proteomics of secretory and endocytic organelles in Giardia lamblia. PLoS ONE |
| title | Proteomics of secretory and endocytic organelles in Giardia lamblia. |
| title_full | Proteomics of secretory and endocytic organelles in Giardia lamblia. |
| title_fullStr | Proteomics of secretory and endocytic organelles in Giardia lamblia. |
| title_full_unstemmed | Proteomics of secretory and endocytic organelles in Giardia lamblia. |
| title_short | Proteomics of secretory and endocytic organelles in Giardia lamblia. |
| title_sort | proteomics of secretory and endocytic organelles in giardia lamblia |
| url | http://europepmc.org/articles/PMC3986054?pdf=render |
| work_keys_str_mv | AT petrabwampfler proteomicsofsecretoryandendocyticorganellesingiardialamblia AT vinkotosevski proteomicsofsecretoryandendocyticorganellesingiardialamblia AT paolonanni proteomicsofsecretoryandendocyticorganellesingiardialamblia AT corneliaspycher proteomicsofsecretoryandendocyticorganellesingiardialamblia AT adrianbhehl proteomicsofsecretoryandendocyticorganellesingiardialamblia |