Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage
IntroductionThe post-harvest period of cowpea [Vigna unguiculata (L) Walp] is marked by substantial losses due to the insect pest Callosobruchus maculatus (Fabricius). The primary goal of the current study is to identify environmentally appropriate substitutes for synthetic pesticides in the managem...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1329100/full |
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| author | Asmae Baghouz Yassir Bouchelta Imane Es-safi Rajae El Brahimi Hamada Imtara Mashail N. AlZain Omer M. Noman Abdelaaty A. Shahat Raja Guemmouh |
| author_facet | Asmae Baghouz Yassir Bouchelta Imane Es-safi Rajae El Brahimi Hamada Imtara Mashail N. AlZain Omer M. Noman Abdelaaty A. Shahat Raja Guemmouh |
| author_sort | Asmae Baghouz |
| collection | DOAJ |
| description | IntroductionThe post-harvest period of cowpea [Vigna unguiculata (L) Walp] is marked by substantial losses due to the insect pest Callosobruchus maculatus (Fabricius). The primary goal of the current study is to identify environmentally appropriate substitutes for synthetic pesticides in the management of stored seed pests. Thus, in a laboratory setting, the insecticidal activity of essential oils (EOs) from Ziziphora hispanica and Satureja calamintha against the cowpea weevil C. maculatus was assessed.MethodsThe fumigant effects of these two EOs were tested with concentrations (4, 8, 12, 16, and 20 μL L−1 of air per 10 g of cowpea seeds) on four biological parameters of C. maculatus: adult mortality, fecundity, fertility, and adult emergence, while concentrations of 4, 12, 16, and 20 μL/cm2 of air were used for the repulsion test.Results and discussionThe fumigant effects of these two EOs were tested with concentrations (4, 8, 12, 16, and 20 μL L−1 of air per 10 g of cowpea seeds) on four biological parameters of C. maculatus: adult mortality, fecundity, fertility, and adult emergence, while concentrations of 4, 12, 16, and 20 μL/cm2 of air were used for the repulsion test. The results of fumigation tests showed a remarkable efficacy of both essential oils against adult C. maculatus after 24 h of exposure. Z. hispanica EO yielded a mortality rate of 80 ± 20%, with an LC50 of 2.77 μL L−1 for males and 66.66 ± 11.54% with an LC50 of 3.57 μL L-1 for females at 4 μL L−1 of air. However, the S. calamintha EO resulted in a mortality rate of 100% for males and 86.66 ± 23.09% with an LC50 of 2.17 μL L−1 for females at low doses. The fecundity was 1.33 ± 0.57 eggs per female. In contrast, this parameter was absent with S. calamintha EO at the low dose, while fertility and emerging adults were missing for both EOs. Furthermore, both EOs showed highly repellent activity towards C. maculatus adults, with 81.66% for Z. hispanica and 91.67% for S. calamintha EO. According to the results of the GC–MS analysis, the primary components of Z. hispanica EO were found to be pulegone (28.17%), alpha-naphtonitrite (10.77%), and 3-(3-thienyl) pro-2-enoic acid (10.62%). Similarly, the main constituents of S. calamintha EO were pulegone (21.48%), piperitenone oxide (17.71%), and eucalyptol (11.99%). Hence, these substances are regarded as the volatile compounds accountable for controlling C. maculatus activities. The study reports that Z. hispanica and S. calamintha show promising fumigant and repellent efficacy and offer new avenues for their potential use as an alternative to synthetic pesticides against stored seed pests. |
| first_indexed | 2024-04-24T10:59:11Z |
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| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-04-24T10:59:11Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-f35ec34cbc2848be87c476d5304b9fc52024-04-12T04:27:51ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2024-04-01810.3389/fsufs.2024.13291001329100Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storageAsmae Baghouz0Yassir Bouchelta1Imane Es-safi2Rajae El Brahimi3Hamada Imtara4Mashail N. AlZain5Omer M. Noman6Abdelaaty A. Shahat7Raja Guemmouh8Laboratory of Biotechnology, Conservation and Valorisation of Naturals Resources (LBCVNR), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fes, MoroccoLaboratory of Soil Microbiology and Environment, Department of Biology, Faculty of Sciences, Moulay Ismail University, Meknes, MoroccoLaboratory of Biotechnology, Environment, Agro-Food, and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah University (USMBA), Fes, MoroccoLaboratory of Biotechnology, Environment, Agro-Food, and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah University (USMBA), Fes, MoroccoFaculty of Sciences, Arab American University, Jenin, PalestineDepartment of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaLaboratory of Biotechnology, Conservation and Valorisation of Naturals Resources (LBCVNR), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fes, MoroccoIntroductionThe post-harvest period of cowpea [Vigna unguiculata (L) Walp] is marked by substantial losses due to the insect pest Callosobruchus maculatus (Fabricius). The primary goal of the current study is to identify environmentally appropriate substitutes for synthetic pesticides in the management of stored seed pests. Thus, in a laboratory setting, the insecticidal activity of essential oils (EOs) from Ziziphora hispanica and Satureja calamintha against the cowpea weevil C. maculatus was assessed.MethodsThe fumigant effects of these two EOs were tested with concentrations (4, 8, 12, 16, and 20 μL L−1 of air per 10 g of cowpea seeds) on four biological parameters of C. maculatus: adult mortality, fecundity, fertility, and adult emergence, while concentrations of 4, 12, 16, and 20 μL/cm2 of air were used for the repulsion test.Results and discussionThe fumigant effects of these two EOs were tested with concentrations (4, 8, 12, 16, and 20 μL L−1 of air per 10 g of cowpea seeds) on four biological parameters of C. maculatus: adult mortality, fecundity, fertility, and adult emergence, while concentrations of 4, 12, 16, and 20 μL/cm2 of air were used for the repulsion test. The results of fumigation tests showed a remarkable efficacy of both essential oils against adult C. maculatus after 24 h of exposure. Z. hispanica EO yielded a mortality rate of 80 ± 20%, with an LC50 of 2.77 μL L−1 for males and 66.66 ± 11.54% with an LC50 of 3.57 μL L-1 for females at 4 μL L−1 of air. However, the S. calamintha EO resulted in a mortality rate of 100% for males and 86.66 ± 23.09% with an LC50 of 2.17 μL L−1 for females at low doses. The fecundity was 1.33 ± 0.57 eggs per female. In contrast, this parameter was absent with S. calamintha EO at the low dose, while fertility and emerging adults were missing for both EOs. Furthermore, both EOs showed highly repellent activity towards C. maculatus adults, with 81.66% for Z. hispanica and 91.67% for S. calamintha EO. According to the results of the GC–MS analysis, the primary components of Z. hispanica EO were found to be pulegone (28.17%), alpha-naphtonitrite (10.77%), and 3-(3-thienyl) pro-2-enoic acid (10.62%). Similarly, the main constituents of S. calamintha EO were pulegone (21.48%), piperitenone oxide (17.71%), and eucalyptol (11.99%). Hence, these substances are regarded as the volatile compounds accountable for controlling C. maculatus activities. The study reports that Z. hispanica and S. calamintha show promising fumigant and repellent efficacy and offer new avenues for their potential use as an alternative to synthetic pesticides against stored seed pests.https://www.frontiersin.org/articles/10.3389/fsufs.2024.1329100/fullcowpeaCallosobruchus maculatusZiziphora hispanicaSatureja calaminthaessential oilsbiological parameters |
| spellingShingle | Asmae Baghouz Yassir Bouchelta Imane Es-safi Rajae El Brahimi Hamada Imtara Mashail N. AlZain Omer M. Noman Abdelaaty A. Shahat Raja Guemmouh Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage Frontiers in Sustainable Food Systems cowpea Callosobruchus maculatus Ziziphora hispanica Satureja calamintha essential oils biological parameters |
| title | Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage |
| title_full | Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage |
| title_fullStr | Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage |
| title_full_unstemmed | Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage |
| title_short | Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage |
| title_sort | biocidal activity of ziziphora hispanica l and satureja calamintha scheele l essential oils against the callosobruchus maculatus fabricius pest on cowpea seeds during storage |
| topic | cowpea Callosobruchus maculatus Ziziphora hispanica Satureja calamintha essential oils biological parameters |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1329100/full |
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