Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria
Free radicals are reactive compounds, their excessive production is considered to be an important cause of oxidative damage in biomolecules causing degenerative diseases. Polyphenols are one of the most important groups of secondary metabolites of plants, which have an antioxidant activity depending...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of Pharmacy University of Baghdad
2021-12-01
|
| Series: | Iraqi Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1533 |
| _version_ | 1818948536899731456 |
|---|---|
| author | Issa Al-Assaf Mays Khazem |
| author_facet | Issa Al-Assaf Mays Khazem |
| author_sort | Issa Al-Assaf |
| collection | DOAJ |
| description | Free radicals are reactive compounds, their excessive production is considered to be an important cause of oxidative damage in biomolecules causing degenerative diseases. Polyphenols are one of the most important groups of secondary metabolites of plants, which have an antioxidant activity depending on their properties as hydrogen donors. Echinops polyceras Boiss. (Asteraceae) is one of Echinops genus species that spread in Syria, Lebanon, and Palestine. Phytochemicals found in this species leaves have been extracted with gradient polarity solvents, and primary screening of the secondary metabolites was established. The phenolic compounds and flavonoids contents were determined. The free radicals scavenging activity was evaluated for all extracts with DPPH• in a 96-well microplate. The specificity study indicates that ascorbic acid was absent, and reducing sugars were exist in the aqueous extract. The identification tests showed the presence of polyphenols like flavonoids and coumarins. The methanolic extract of the E. polyceras leaves was the most effective scavengers of free radicals (90.22% in 30 min) with phenolic compounds content 682.5 mg GAE/g of dried extract (DE) and flavonoids content 194.5 mg QE/ g DE. The chloroform extract was the least effective as free radical scavenging (60% in 30 min) as the phenolic compounds content was 278.5 mg GAE/g DE and flavonoids content 94 mg QE/ g DE. In conclusion, the phenolic compounds and flavonoids from Echinops polyceras Boiss. are effective in free radicals scavenging and protecting from diseases caused by oxidative stress. |
| first_indexed | 2024-12-20T08:48:22Z |
| format | Article |
| id | doaj.art-f35f8de79d7c4ec88843036e5296171b |
| institution | Directory Open Access Journal |
| issn | 2521-3512 1683-3597 |
| language | English |
| last_indexed | 2024-12-20T08:48:22Z |
| publishDate | 2021-12-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-f35f8de79d7c4ec88843036e5296171b2022-12-21T19:46:12ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences2521-35121683-35972021-12-0130210.31351/vol30iss2pp261-268Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in SyriaIssa Al-Assaf0Mays Khazem1Department of Pharmacognosy in Faculty of Pharmacy, Damascus University, Damascus, SyriaDepartment of Pharmacognosy in Faculty of Pharmacy, Damascus University, Damascus, SyriaFree radicals are reactive compounds, their excessive production is considered to be an important cause of oxidative damage in biomolecules causing degenerative diseases. Polyphenols are one of the most important groups of secondary metabolites of plants, which have an antioxidant activity depending on their properties as hydrogen donors. Echinops polyceras Boiss. (Asteraceae) is one of Echinops genus species that spread in Syria, Lebanon, and Palestine. Phytochemicals found in this species leaves have been extracted with gradient polarity solvents, and primary screening of the secondary metabolites was established. The phenolic compounds and flavonoids contents were determined. The free radicals scavenging activity was evaluated for all extracts with DPPH• in a 96-well microplate. The specificity study indicates that ascorbic acid was absent, and reducing sugars were exist in the aqueous extract. The identification tests showed the presence of polyphenols like flavonoids and coumarins. The methanolic extract of the E. polyceras leaves was the most effective scavengers of free radicals (90.22% in 30 min) with phenolic compounds content 682.5 mg GAE/g of dried extract (DE) and flavonoids content 194.5 mg QE/ g DE. The chloroform extract was the least effective as free radical scavenging (60% in 30 min) as the phenolic compounds content was 278.5 mg GAE/g DE and flavonoids content 94 mg QE/ g DE. In conclusion, the phenolic compounds and flavonoids from Echinops polyceras Boiss. are effective in free radicals scavenging and protecting from diseases caused by oxidative stress.https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1533Antioxidants, Polyphenols, Flavonoids, Free Radicals, and Echinops polyceras Boiss |
| spellingShingle | Issa Al-Assaf Mays Khazem Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria Iraqi Journal of Pharmaceutical Sciences Antioxidants, Polyphenols, Flavonoids, Free Radicals, and Echinops polyceras Boiss |
| title | Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria |
| title_full | Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria |
| title_fullStr | Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria |
| title_full_unstemmed | Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria |
| title_short | Antioxidant Activity of Total phenols and Flavonoids extracted from Echinops polyceras roots grown in Syria |
| title_sort | antioxidant activity of total phenols and flavonoids extracted from echinops polyceras roots grown in syria |
| topic | Antioxidants, Polyphenols, Flavonoids, Free Radicals, and Echinops polyceras Boiss |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1533 |
| work_keys_str_mv | AT issaalassaf antioxidantactivityoftotalphenolsandflavonoidsextractedfromechinopspolycerasrootsgrowninsyria AT mayskhazem antioxidantactivityoftotalphenolsandflavonoidsextractedfromechinopspolycerasrootsgrowninsyria |