Detection of viruses in the Lake of Maracaibo

A methodology for concentrating viruses In water was used In a six month study to detect the presence ofenteroviruses In an area of Lake Maracaibo. Concentrated samples were Inoculated In BGM, MA-}04 and YERO cell lInes. The positive Iso lates for cytophatic effect were analyzed In order to determin...

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Bibliographic Details
Main Authors: Ligia Botero, Giuliana malaro, Rosalva Salas, Hugo Hernández
Format: Article
Language:English
Published: Universidad del Zulia 2011-01-01
Series:Revista Técnica de la Facultad de Ingeniería
Subjects:
Online Access:https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5509
Description
Summary:A methodology for concentrating viruses In water was used In a six month study to detect the presence ofenteroviruses In an area of Lake Maracaibo. Concentrated samples were Inoculated In BGM, MA-}04 and YERO cell lInes. The positive Iso lates for cytophatic effect were analyzed In order to determine fue heat stabllization of divalent cations and later Identified using a neutralization technique With LB-M serum. The samples analyzed proved 100% positive for enteroviruses. This Indicates that the area studied was highly contaminated. 1\velve were Identified as pollovirus 2 and two as echovirus 21. Six of the samples gave results that to not correspond to any of the neutralizing combinations and two Isolates did not neutralize any serum combination.
ISSN:0254-0770
2477-9377