Detection of viruses in the Lake of Maracaibo
A methodology for concentrating viruses In water was used In a six month study to detect the presence ofenteroviruses In an area of Lake Maracaibo. Concentrated samples were Inoculated In BGM, MA-}04 and YERO cell lInes. The positive Iso lates for cytophatic effect were analyzed In order to determin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidad del Zulia
2011-01-01
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| Series: | Revista Técnica de la Facultad de Ingeniería |
| Subjects: | |
| Online Access: | https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5509 |
| _version_ | 1811188547951001600 |
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| author | Ligia Botero Giuliana malaro Rosalva Salas Hugo Hernández |
| author_facet | Ligia Botero Giuliana malaro Rosalva Salas Hugo Hernández |
| author_sort | Ligia Botero |
| collection | DOAJ |
| description | A methodology for concentrating viruses In water was used In a six month study to detect the presence ofenteroviruses In an area of Lake Maracaibo. Concentrated samples were Inoculated In BGM, MA-}04 and YERO cell lInes. The positive Iso lates for cytophatic effect were analyzed In order to determine fue heat stabllization of divalent cations and later Identified using a neutralization technique With LB-M serum. The samples analyzed proved 100% positive for enteroviruses. This Indicates that the area studied was highly contaminated. 1\velve were Identified as pollovirus 2 and two as echovirus 21. Six of the samples gave results that to not correspond to any of the neutralizing combinations and two Isolates did not neutralize any serum combination.
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| first_indexed | 2024-04-11T14:21:43Z |
| format | Article |
| id | doaj.art-f360d20e617b4f06be120011c20ed284 |
| institution | Directory Open Access Journal |
| issn | 0254-0770 2477-9377 |
| language | English |
| last_indexed | 2024-04-11T14:21:43Z |
| publishDate | 2011-01-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Técnica de la Facultad de Ingeniería |
| spelling | doaj.art-f360d20e617b4f06be120011c20ed2842022-12-22T04:19:03ZengUniversidad del ZuliaRevista Técnica de la Facultad de Ingeniería0254-07702477-93772011-01-01192Detection of viruses in the Lake of MaracaiboLigia Botero0Giuliana malaro1Rosalva Salas2Hugo Hernández3Universidad del Zulia-VenezuelaUniversidad del Zulia-VeneuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaA methodology for concentrating viruses In water was used In a six month study to detect the presence ofenteroviruses In an area of Lake Maracaibo. Concentrated samples were Inoculated In BGM, MA-}04 and YERO cell lInes. The positive Iso lates for cytophatic effect were analyzed In order to determine fue heat stabllization of divalent cations and later Identified using a neutralization technique With LB-M serum. The samples analyzed proved 100% positive for enteroviruses. This Indicates that the area studied was highly contaminated. 1\velve were Identified as pollovirus 2 and two as echovirus 21. Six of the samples gave results that to not correspond to any of the neutralizing combinations and two Isolates did not neutralize any serum combination. https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5509virusenteroviruslakeestuarypolloviruswater |
| spellingShingle | Ligia Botero Giuliana malaro Rosalva Salas Hugo Hernández Detection of viruses in the Lake of Maracaibo Revista Técnica de la Facultad de Ingeniería virus enterovirus lake estuary pollovirus water |
| title | Detection of viruses in the Lake of Maracaibo |
| title_full | Detection of viruses in the Lake of Maracaibo |
| title_fullStr | Detection of viruses in the Lake of Maracaibo |
| title_full_unstemmed | Detection of viruses in the Lake of Maracaibo |
| title_short | Detection of viruses in the Lake of Maracaibo |
| title_sort | detection of viruses in the lake of maracaibo |
| topic | virus enterovirus lake estuary pollovirus water |
| url | https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5509 |
| work_keys_str_mv | AT ligiabotero detectionofvirusesinthelakeofmaracaibo AT giulianamalaro detectionofvirusesinthelakeofmaracaibo AT rosalvasalas detectionofvirusesinthelakeofmaracaibo AT hugohernandez detectionofvirusesinthelakeofmaracaibo |