Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure
Introduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1154454/full |
| _version_ | 1797861053778886656 |
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| author | Patricia E. McCallinhart Patricia E. McCallinhart Yong Ung Lee Yong Ung Lee Avione Lee Avione Lee Mircea Anghelescu Jeffrey R. Tonniges Ed Calomeni Gunjan Agarwal Gunjan Agarwal Joy Lincoln Joy Lincoln Joy Lincoln Aaron J. Trask Aaron J. Trask Aaron J. Trask Aaron J. Trask |
| author_facet | Patricia E. McCallinhart Patricia E. McCallinhart Yong Ung Lee Yong Ung Lee Avione Lee Avione Lee Mircea Anghelescu Jeffrey R. Tonniges Ed Calomeni Gunjan Agarwal Gunjan Agarwal Joy Lincoln Joy Lincoln Joy Lincoln Aaron J. Trask Aaron J. Trask Aaron J. Trask Aaron J. Trask |
| author_sort | Patricia E. McCallinhart |
| collection | DOAJ |
| description | Introduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that increased aortic PWV in type 2 diabetic mice is driven by increased blood pressure rather than vascular biomechanics.Methods and Results: Using a combination of in vivo PWV and ex vivo pressure myography, our data demonstrate no difference in ex vivo passive mechanics, including outer diameter, inner diameter, compliance (Db/db: 0.0094 ± 0.0018 mm2/mmHg vs. db/db: 0.0080 ± 0.0008 mm2/mmHg, p > 0.05 at 100 mmHg), and incremental modulus (Db/db: 801.52 ± 135.87 kPa vs. db/db: 838.12 ± 44.90 kPa, p > 0.05 at 100 mmHg), in normal versus diabetic 16 week old mice. We further report no difference in basal or active aorta biomechanics in normal versus diabetic 16 week old mice. Finally, we show here that the increase in diabetic in vivo aortic pulse wave velocity at baseline was completely abolished when measured at equivalent pharmacologically-modulated blood pressures, indicating that the elevated PWV was attributed to the concomitant increase in blood pressure at baseline, and therefore “stiffness.”Conclusions: Together, these animal model data suggest an intimate regulation of blood pressure during collection of pulse wave velocity when determining in vivo vascular stiffness. These data further indicate caution should be exerted when interpreting elevated PWV as the pure marker of vascular stiffness. |
| first_indexed | 2024-04-09T21:55:52Z |
| format | Article |
| id | doaj.art-f36109b6a04c458a91c0bf2d4c1b32e7 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-09T21:55:52Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-f36109b6a04c458a91c0bf2d4c1b32e72023-03-24T06:01:57ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-03-011410.3389/fphys.2023.11544541154454Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressurePatricia E. McCallinhart0Patricia E. McCallinhart1Yong Ung Lee2Yong Ung Lee3Avione Lee4Avione Lee5Mircea Anghelescu6Jeffrey R. Tonniges7Ed Calomeni8Gunjan Agarwal9Gunjan Agarwal10Joy Lincoln11Joy Lincoln12Joy Lincoln13Aaron J. Trask14Aaron J. Trask15Aaron J. Trask16Aaron J. Trask17Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, United StatesCenter for Cardiovascular Research, Nationwide Children’s Hospital, Columbus, OH, United StatesAbigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, United StatesTissue Engineering Program and Surgical Research, Columbus, OH, United StatesAbigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, United StatesTissue Engineering Program and Surgical Research, Columbus, OH, United StatesDepartment of Biomedical Sciences, Philadelphia College of Osteopathic Medicine (PCOM), Suwanee, GA, United StatesBiophysics Graduate Program at The Ohio State University, Columbus, OH, United StatesDorothy M. Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH, United StatesBiophysics Graduate Program at The Ohio State University, Columbus, OH, United StatesDorothy M. Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH, United StatesAbigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, United StatesCenter for Cardiovascular Research, Nationwide Children’s Hospital, Columbus, OH, United StatesDepartment of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, United StatesAbigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, United StatesCenter for Cardiovascular Research, Nationwide Children’s Hospital, Columbus, OH, United StatesDorothy M. Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH, United StatesDepartment of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, United StatesIntroduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that increased aortic PWV in type 2 diabetic mice is driven by increased blood pressure rather than vascular biomechanics.Methods and Results: Using a combination of in vivo PWV and ex vivo pressure myography, our data demonstrate no difference in ex vivo passive mechanics, including outer diameter, inner diameter, compliance (Db/db: 0.0094 ± 0.0018 mm2/mmHg vs. db/db: 0.0080 ± 0.0008 mm2/mmHg, p > 0.05 at 100 mmHg), and incremental modulus (Db/db: 801.52 ± 135.87 kPa vs. db/db: 838.12 ± 44.90 kPa, p > 0.05 at 100 mmHg), in normal versus diabetic 16 week old mice. We further report no difference in basal or active aorta biomechanics in normal versus diabetic 16 week old mice. Finally, we show here that the increase in diabetic in vivo aortic pulse wave velocity at baseline was completely abolished when measured at equivalent pharmacologically-modulated blood pressures, indicating that the elevated PWV was attributed to the concomitant increase in blood pressure at baseline, and therefore “stiffness.”Conclusions: Together, these animal model data suggest an intimate regulation of blood pressure during collection of pulse wave velocity when determining in vivo vascular stiffness. These data further indicate caution should be exerted when interpreting elevated PWV as the pure marker of vascular stiffness.https://www.frontiersin.org/articles/10.3389/fphys.2023.1154454/fullvascular stiffnesspulse wave velocityhypertensiondiabetesmetabolic syndromeaortic biomechanics |
| spellingShingle | Patricia E. McCallinhart Patricia E. McCallinhart Yong Ung Lee Yong Ung Lee Avione Lee Avione Lee Mircea Anghelescu Jeffrey R. Tonniges Ed Calomeni Gunjan Agarwal Gunjan Agarwal Joy Lincoln Joy Lincoln Joy Lincoln Aaron J. Trask Aaron J. Trask Aaron J. Trask Aaron J. Trask Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure Frontiers in Physiology vascular stiffness pulse wave velocity hypertension diabetes metabolic syndrome aortic biomechanics |
| title | Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure |
| title_full | Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure |
| title_fullStr | Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure |
| title_full_unstemmed | Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure |
| title_short | Dissociation of pulse wave velocity and aortic wall stiffness in diabetic db/db mice: The influence of blood pressure |
| title_sort | dissociation of pulse wave velocity and aortic wall stiffness in diabetic db db mice the influence of blood pressure |
| topic | vascular stiffness pulse wave velocity hypertension diabetes metabolic syndrome aortic biomechanics |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1154454/full |
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