Preparation and Biological Activities of Low Molecular Weight Brown Algae
The polysaccharide was degraded by ascorbic acid and hydrogen peroxide, and the optimal degradation conditions were obtained by the index of DPPH free radical scavenging rate. Then the degradation products were classified by ultrafiltration to obtain different molecular weight components, and their...
| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050125 |
| _version_ | 1798022884760748032 |
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| author | Fei QUE Wenjing TAO Wenjie FENG |
| author_facet | Fei QUE Wenjing TAO Wenjie FENG |
| author_sort | Fei QUE |
| collection | DOAJ |
| description | The polysaccharide was degraded by ascorbic acid and hydrogen peroxide, and the optimal degradation conditions were obtained by the index of DPPH free radical scavenging rate. Then the degradation products were classified by ultrafiltration to obtain different molecular weight components, and their activities were analyzed. The optimal degradation conditions were H2O2-VC 20 mmol/L, 45 ℃ and 3 h. Under these conditions, the DPPH free radical scavenging rate reached 61.23%, and the yield of degradation products was 73.16%. Electrophoretic results showed that the bands of the degraded polysaccharides were obviously in the low molecular weight region. Then the degradation products were classified into four different molecular weight fractions, as <5 kDa, 5~10 kDa, 10~30 kDa and >30 kDa by ultrafiltration. There were significant differences among the molecular weight segments (P<0.05), especially the <5 kDa component (main component 2.140×103 Da, 29.6%) had the best biological activity. The DPPH free radical scavenging rate was 59.27%, and the moisture retention rate was 75.75% after 60 h, the tyrosinase inhibition rate was 65.28%. The content of uronic acid of the <5 kDa component decreased slightly compared with polysaccharides. The results could provide theoretical basis for the application of polysaccharides in functional food and other fields. |
| first_indexed | 2024-04-11T17:37:15Z |
| format | Article |
| id | doaj.art-f362df3e22474da8a4ed8f8f1fdcba8f |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T17:37:15Z |
| publishDate | 2022-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-f362df3e22474da8a4ed8f8f1fdcba8f2022-12-22T04:11:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-01-0143222623210.13386/j.issn1002-0306.20210501252021050125-2Preparation and Biological Activities of Low Molecular Weight Brown AlgaeFei QUE0Wenjing TAO1Wenjie FENG2College of Applied Engineering, Zhejiang Institute of Economics and Trade, Hangzhou 310018, ChinaBeijing Meizheng Bio-Tech Co., Ltd., Beijing 102200, ChinaCollege of Applied Engineering, Zhejiang Institute of Economics and Trade, Hangzhou 310018, ChinaThe polysaccharide was degraded by ascorbic acid and hydrogen peroxide, and the optimal degradation conditions were obtained by the index of DPPH free radical scavenging rate. Then the degradation products were classified by ultrafiltration to obtain different molecular weight components, and their activities were analyzed. The optimal degradation conditions were H2O2-VC 20 mmol/L, 45 ℃ and 3 h. Under these conditions, the DPPH free radical scavenging rate reached 61.23%, and the yield of degradation products was 73.16%. Electrophoretic results showed that the bands of the degraded polysaccharides were obviously in the low molecular weight region. Then the degradation products were classified into four different molecular weight fractions, as <5 kDa, 5~10 kDa, 10~30 kDa and >30 kDa by ultrafiltration. There were significant differences among the molecular weight segments (P<0.05), especially the <5 kDa component (main component 2.140×103 Da, 29.6%) had the best biological activity. The DPPH free radical scavenging rate was 59.27%, and the moisture retention rate was 75.75% after 60 h, the tyrosinase inhibition rate was 65.28%. The content of uronic acid of the <5 kDa component decreased slightly compared with polysaccharides. The results could provide theoretical basis for the application of polysaccharides in functional food and other fields.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050125low molecular weight brown algaepreparationdpph free radical scavenging ratethe tyrosinase inhibition ratemoisture retention rate |
| spellingShingle | Fei QUE Wenjing TAO Wenjie FENG Preparation and Biological Activities of Low Molecular Weight Brown Algae Shipin gongye ke-ji low molecular weight brown algae preparation dpph free radical scavenging rate the tyrosinase inhibition rate moisture retention rate |
| title | Preparation and Biological Activities of Low Molecular Weight Brown Algae |
| title_full | Preparation and Biological Activities of Low Molecular Weight Brown Algae |
| title_fullStr | Preparation and Biological Activities of Low Molecular Weight Brown Algae |
| title_full_unstemmed | Preparation and Biological Activities of Low Molecular Weight Brown Algae |
| title_short | Preparation and Biological Activities of Low Molecular Weight Brown Algae |
| title_sort | preparation and biological activities of low molecular weight brown algae |
| topic | low molecular weight brown algae preparation dpph free radical scavenging rate the tyrosinase inhibition rate moisture retention rate |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050125 |
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