Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice
Background: Thymoquinone as the main active component of Nigella sativa might have a various pharmacological effects such as antiapoptotic and antioxidant. Morphine is commonly used for the treatment of severe pain that can increase the generation of free radicals and affects the spermatogenesis. Th...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2018;volume=7;issue=1;spage=18;epage=18;aulast=Salahshoor |
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| author | Mohammad Reza Salahshoor Mojdeh Haghjoo Shiva Roshankhah Fatemeh Makalani Cyrus Jalili |
| author_facet | Mohammad Reza Salahshoor Mojdeh Haghjoo Shiva Roshankhah Fatemeh Makalani Cyrus Jalili |
| author_sort | Mohammad Reza Salahshoor |
| collection | DOAJ |
| description | Background: Thymoquinone as the main active component of Nigella sativa might have a various pharmacological effects such as antiapoptotic and antioxidant. Morphine is commonly used for the treatment of severe pain that can increase the generation of free radicals and affects the spermatogenesis. This study was designed to evaluate protective effects of thymoquinone against morphine-induced damages, sperm viability, count, motility, morphology and testis histology, and nitric oxide and testosterone hormone of the mice. Materials and Methods: In this experimental study, we divided 48 mice into eight groups (n = 6); various doses of thymoquinone (2, 10, and 20 mg/kg) and morphine (20 mg/kg) plus thymoquinone (2, 10, and 20 mg/kg) were administered intraperitoneally to 48 male mice for 30 consequent days. Male reproductive parameters including testis weight, testosterone hormone, serum nitric oxide, germinal thickness, sperm morphology, count, viability, and motility were analyzed and compared. Results: The results indicated that morphine administration significantly decreased germinal thickness, testis weight, testosterone level, viability, morphology, count, and motility of sperm and increased nitric oxide as compared to saline group (P < 0.05). However, increasing the dose of thymoquinone in the thymoquinone and thymoquinone plus morphine groups significantly decreases nitric oxide level (P < 0.05) while significantly boosted motility, morphology, count, viability of sperm cells, germinal thickness, and testosterone hormone in all groups as compared to morphine group (P < 0.05). Conclusion: It seems that thymoquinone administration could increase the quality some of spermatozoa and improves morphine-induced adverse effects on reproductive parameters in male mice |
| first_indexed | 2024-12-21T12:42:19Z |
| format | Article |
| id | doaj.art-f364a572b0754ef7aeada29d26c41833 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 |
| language | English |
| last_indexed | 2024-12-21T12:42:19Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-f364a572b0754ef7aeada29d26c418332022-12-21T19:03:44ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752018-01-0171181810.4103/abr.abr_69_17Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male MiceMohammad Reza SalahshoorMojdeh HaghjooShiva RoshankhahFatemeh MakalaniCyrus JaliliBackground: Thymoquinone as the main active component of Nigella sativa might have a various pharmacological effects such as antiapoptotic and antioxidant. Morphine is commonly used for the treatment of severe pain that can increase the generation of free radicals and affects the spermatogenesis. This study was designed to evaluate protective effects of thymoquinone against morphine-induced damages, sperm viability, count, motility, morphology and testis histology, and nitric oxide and testosterone hormone of the mice. Materials and Methods: In this experimental study, we divided 48 mice into eight groups (n = 6); various doses of thymoquinone (2, 10, and 20 mg/kg) and morphine (20 mg/kg) plus thymoquinone (2, 10, and 20 mg/kg) were administered intraperitoneally to 48 male mice for 30 consequent days. Male reproductive parameters including testis weight, testosterone hormone, serum nitric oxide, germinal thickness, sperm morphology, count, viability, and motility were analyzed and compared. Results: The results indicated that morphine administration significantly decreased germinal thickness, testis weight, testosterone level, viability, morphology, count, and motility of sperm and increased nitric oxide as compared to saline group (P < 0.05). However, increasing the dose of thymoquinone in the thymoquinone and thymoquinone plus morphine groups significantly decreases nitric oxide level (P < 0.05) while significantly boosted motility, morphology, count, viability of sperm cells, germinal thickness, and testosterone hormone in all groups as compared to morphine group (P < 0.05). Conclusion: It seems that thymoquinone administration could increase the quality some of spermatozoa and improves morphine-induced adverse effects on reproductive parameters in male micehttp://www.advbiores.net/article.asp?issn=2277-9175;year=2018;volume=7;issue=1;spage=18;epage=18;aulast=SalahshoorMorphinereproductivethymoquinone |
| spellingShingle | Mohammad Reza Salahshoor Mojdeh Haghjoo Shiva Roshankhah Fatemeh Makalani Cyrus Jalili Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice Advanced Biomedical Research Morphine reproductive thymoquinone |
| title | Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice |
| title_full | Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice |
| title_fullStr | Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice |
| title_full_unstemmed | Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice |
| title_short | Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice |
| title_sort | effect of thymoquinone on reproductive parameter in morphine treated male mice |
| topic | Morphine reproductive thymoquinone |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2018;volume=7;issue=1;spage=18;epage=18;aulast=Salahshoor |
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