Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants
This study determines the phytochemical contents of selected medicinal plant extracts quantitatively using spectrophotometry and to evaluate their safety on mammalian cells in vitro using tetrazolium-based colorimetric cytotoxicity assay. Twenty-two ethanolic plant extracts were assessed, and the re...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Journal of Applied Animal Research |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09712119.2022.2119979 |
| _version_ | 1817985729779728384 |
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| author | Aderonke N. Abd’quadri-Abojukoro Sanah M. Nkadimeng Lyndy J. McGaw Ignatius V. Nsahlai |
| author_facet | Aderonke N. Abd’quadri-Abojukoro Sanah M. Nkadimeng Lyndy J. McGaw Ignatius V. Nsahlai |
| author_sort | Aderonke N. Abd’quadri-Abojukoro |
| collection | DOAJ |
| description | This study determines the phytochemical contents of selected medicinal plant extracts quantitatively using spectrophotometry and to evaluate their safety on mammalian cells in vitro using tetrazolium-based colorimetric cytotoxicity assay. Twenty-two ethanolic plant extracts were assessed, and the result showed that Psidium guajava had the highest concentration of alkaloids (219.06±11.50mg/g dry-weight (dw)). In contrast, Acacia nilotica leaf extract with 191.60±9.07mg/gdw had the highest level of flavonoids. Vernonia amygdalina and Moringa oleifera contained more steroids than the other plant extracts while condensed tannin was highest in Carya illinoinensis (21.72 ± 0.84 mg/g dw). Coffea arabica, Acacia nilotica leaf, Vernonia amygdalina, Carya illinoinensis and Psidium guajava had more saponins than the other plant extracts. Regarding cytotoxicity evaluation, Allium cepa and Tulbaghia violacea with concentration killing 50% of cells (LC50) values of 0.5182±0.40 and 0.4909 ± 0.034 mg/ml, respectively, were the safest of all the plant extracts. Acacia nilotica pod and Camelia japonica leaf extracts were the most cytotoxic with LC50 values of 0.0101 ± 0.016 and 0.0151 ± 0.005 mg/ml, respectively, which are well below the recommended toxic cut-off point (0.03mg/ml). The best selectivity index (SI) value of 1.061 was obtained with Aloe ferox against S. aureus, which is a Gram-positive bacterium. |
| first_indexed | 2024-04-14T00:00:45Z |
| format | Article |
| id | doaj.art-f368c142f8074107919bf59bc7010a5e |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-04-14T00:00:45Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-f368c142f8074107919bf59bc7010a5e2022-12-22T02:23:42ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442022-12-0150165666510.1080/09712119.2022.2119979Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plantsAderonke N. Abd’quadri-Abojukoro0Sanah M. Nkadimeng1Lyndy J. McGaw2Ignatius V. Nsahlai3Discipline of Animal and Poultry Science, University of KwaZulu-Natal, Scottsville, South AfricaPhytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, Onderstepoort, South AfricaPhytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, Onderstepoort, South AfricaDiscipline of Animal and Poultry Science, University of KwaZulu-Natal, Scottsville, South AfricaThis study determines the phytochemical contents of selected medicinal plant extracts quantitatively using spectrophotometry and to evaluate their safety on mammalian cells in vitro using tetrazolium-based colorimetric cytotoxicity assay. Twenty-two ethanolic plant extracts were assessed, and the result showed that Psidium guajava had the highest concentration of alkaloids (219.06±11.50mg/g dry-weight (dw)). In contrast, Acacia nilotica leaf extract with 191.60±9.07mg/gdw had the highest level of flavonoids. Vernonia amygdalina and Moringa oleifera contained more steroids than the other plant extracts while condensed tannin was highest in Carya illinoinensis (21.72 ± 0.84 mg/g dw). Coffea arabica, Acacia nilotica leaf, Vernonia amygdalina, Carya illinoinensis and Psidium guajava had more saponins than the other plant extracts. Regarding cytotoxicity evaluation, Allium cepa and Tulbaghia violacea with concentration killing 50% of cells (LC50) values of 0.5182±0.40 and 0.4909 ± 0.034 mg/ml, respectively, were the safest of all the plant extracts. Acacia nilotica pod and Camelia japonica leaf extracts were the most cytotoxic with LC50 values of 0.0101 ± 0.016 and 0.0151 ± 0.005 mg/ml, respectively, which are well below the recommended toxic cut-off point (0.03mg/ml). The best selectivity index (SI) value of 1.061 was obtained with Aloe ferox against S. aureus, which is a Gram-positive bacterium.https://www.tandfonline.com/doi/10.1080/09712119.2022.2119979Rumen metabolismphytochemicalsmethane emissionplant extractscytotoxicity |
| spellingShingle | Aderonke N. Abd’quadri-Abojukoro Sanah M. Nkadimeng Lyndy J. McGaw Ignatius V. Nsahlai Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants Journal of Applied Animal Research Rumen metabolism phytochemicals methane emission plant extracts cytotoxicity |
| title | Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants |
| title_full | Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants |
| title_fullStr | Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants |
| title_full_unstemmed | Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants |
| title_short | Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants |
| title_sort | phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants |
| topic | Rumen metabolism phytochemicals methane emission plant extracts cytotoxicity |
| url | https://www.tandfonline.com/doi/10.1080/09712119.2022.2119979 |
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