In vivo production of pederin by labrenzin pathway expansion
Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus Paederus. Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynth...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | Metabolic Engineering Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030122000074 |
| _version_ | 1811231579414986752 |
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| author | Dina Kačar Carmen Schleissner Librada M. Cañedo Pilar Rodríguez Fernando de la Calle Carmen Cuevas Beatriz Galán José Luis García |
| author_facet | Dina Kačar Carmen Schleissner Librada M. Cañedo Pilar Rodríguez Fernando de la Calle Carmen Cuevas Beatriz Galán José Luis García |
| author_sort | Dina Kačar |
| collection | DOAJ |
| description | Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus Paederus. Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium Labrenzia sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic pedO gene encoding one of the three O-methyltraferase of the pederin cluster into Labrenzia sp. PHM005 we have been able to produce pederin for the first time by fermentation in the new recombinant strain. |
| first_indexed | 2024-04-12T10:47:23Z |
| format | Article |
| id | doaj.art-f3696ccacf6d407e99a8d33654b4e88d |
| institution | Directory Open Access Journal |
| issn | 2214-0301 |
| language | English |
| last_indexed | 2024-04-12T10:47:23Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj.art-f3696ccacf6d407e99a8d33654b4e88d2022-12-22T03:36:23ZengElsevierMetabolic Engineering Communications2214-03012022-06-0114e00198In vivo production of pederin by labrenzin pathway expansionDina Kačar0Carmen Schleissner1Librada M. Cañedo2Pilar Rodríguez3Fernando de la Calle4Carmen Cuevas5Beatriz Galán6José Luis García7Department of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas, Agencia Estatal Consejo Superior de Investigaciones Científicas, Madrid, SpainResearch and Development Department, PharmaMar S.A, Madrid, SpainResearch and Development Department, PharmaMar S.A, Madrid, SpainResearch and Development Department, PharmaMar S.A, Madrid, SpainResearch and Development Department, PharmaMar S.A, Madrid, SpainResearch and Development Department, PharmaMar S.A, Madrid, SpainDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas, Agencia Estatal Consejo Superior de Investigaciones Científicas, Madrid, SpainDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas, Agencia Estatal Consejo Superior de Investigaciones Científicas, Madrid, Spain; Corresponding author.Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus Paederus. Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium Labrenzia sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic pedO gene encoding one of the three O-methyltraferase of the pederin cluster into Labrenzia sp. PHM005 we have been able to produce pederin for the first time by fermentation in the new recombinant strain.http://www.sciencedirect.com/science/article/pii/S2214030122000074 |
| spellingShingle | Dina Kačar Carmen Schleissner Librada M. Cañedo Pilar Rodríguez Fernando de la Calle Carmen Cuevas Beatriz Galán José Luis García In vivo production of pederin by labrenzin pathway expansion Metabolic Engineering Communications |
| title | In vivo production of pederin by labrenzin pathway expansion |
| title_full | In vivo production of pederin by labrenzin pathway expansion |
| title_fullStr | In vivo production of pederin by labrenzin pathway expansion |
| title_full_unstemmed | In vivo production of pederin by labrenzin pathway expansion |
| title_short | In vivo production of pederin by labrenzin pathway expansion |
| title_sort | in vivo production of pederin by labrenzin pathway expansion |
| url | http://www.sciencedirect.com/science/article/pii/S2214030122000074 |
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