In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity
Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that B...
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MDPI AG
2021-12-01
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| author | Yeon-Seop Jung So-Hee Lee So Young Chun Dae Hwan Kim Byung Ik Jang Man-Hoon Han Syng-Ook Lee |
| author_facet | Yeon-Seop Jung So-Hee Lee So Young Chun Dae Hwan Kim Byung Ik Jang Man-Hoon Han Syng-Ook Lee |
| author_sort | Yeon-Seop Jung |
| collection | DOAJ |
| description | Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that BLE might protect liver cells against oxidative stress-induced cytotoxicity. BLE was evaluated for its protective effects against oxidative stress-induced hepatotoxicity in AML12 mouse hepatocytes and BALB/c mice. H<sub>2</sub>O<sub>2</sub> treatment caused a marked decrease in cell viability; however, pretreatment with BLE (25–100 μg/mL) for 24 h significantly preserved the viability of H<sub>2</sub>O<sub>2</sub>-treated cells up to about 50% at 100 μg/mL. As expected, BLE dramatically reduced intracellular reactive oxygen species (ROS) levels in a dose-dependent manner in H<sub>2</sub>O<sub>2</sub>-treated cells. Further mechanistic studies demonstrated that BLE reduced cellular ROS levels, partly by increasing expression of antioxidant genes. Furthermore, pretreatment with BLE (400 mg/kg) for 2 weeks significantly reduced serum levels of alanine transaminase and triglyceride by about 49% and 40%, respectively, and increased the expression and activity of glutathione peroxidase in CCl<sub>4</sub>-treated BALB/c mice. These results suggest that BLE protects liver cells against oxidative stress, partly by inducing cellular antioxidant system; thus, it represents a potential source of nutraceuticals with hepatoprotective effects. |
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| spelling | doaj.art-f36ca1e2846241868422a24fe59314742023-11-23T11:56:06ZengMDPI AGMolecules1420-30492021-12-012715910.3390/molecules27010059In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced HepatotoxicityYeon-Seop Jung0So-Hee Lee1So Young Chun2Dae Hwan Kim3Byung Ik Jang4Man-Hoon Han5Syng-Ook Lee6Department of Food Science and Technology, Keimyung University, Daegu 42601, KoreaDepartment of Food Science and Technology, Keimyung University, Daegu 42601, KoreaBioMedical Research Institute, Kyungpook National University Hospital, Daegu 41404, KoreaDepartment of Laboratory Animal Research Support Team, Yeungnam University Medical Center, Daegu 42415, KoreaDepartment of Internal Medicine, Yeungnam University College of Medicine, Daegu 42415, KoreaDepartment of Pathology, School of Medicine, Kyungpook National University, Daegu 41404, KoreaDepartment of Food Science and Technology, Keimyung University, Daegu 42601, KoreaExcessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that BLE might protect liver cells against oxidative stress-induced cytotoxicity. BLE was evaluated for its protective effects against oxidative stress-induced hepatotoxicity in AML12 mouse hepatocytes and BALB/c mice. H<sub>2</sub>O<sub>2</sub> treatment caused a marked decrease in cell viability; however, pretreatment with BLE (25–100 μg/mL) for 24 h significantly preserved the viability of H<sub>2</sub>O<sub>2</sub>-treated cells up to about 50% at 100 μg/mL. As expected, BLE dramatically reduced intracellular reactive oxygen species (ROS) levels in a dose-dependent manner in H<sub>2</sub>O<sub>2</sub>-treated cells. Further mechanistic studies demonstrated that BLE reduced cellular ROS levels, partly by increasing expression of antioxidant genes. Furthermore, pretreatment with BLE (400 mg/kg) for 2 weeks significantly reduced serum levels of alanine transaminase and triglyceride by about 49% and 40%, respectively, and increased the expression and activity of glutathione peroxidase in CCl<sub>4</sub>-treated BALB/c mice. These results suggest that BLE protects liver cells against oxidative stress, partly by inducing cellular antioxidant system; thus, it represents a potential source of nutraceuticals with hepatoprotective effects.https://www.mdpi.com/1420-3049/27/1/59lentilhepatoprotective effectoxidative stressNrf2 |
| spellingShingle | Yeon-Seop Jung So-Hee Lee So Young Chun Dae Hwan Kim Byung Ik Jang Man-Hoon Han Syng-Ook Lee In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity Molecules lentil hepatoprotective effect oxidative stress Nrf2 |
| title | In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity |
| title_full | In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity |
| title_fullStr | In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity |
| title_full_unstemmed | In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity |
| title_short | In Vitro and In Vivo Protective Effects of Lentil (<i>Lens culinaris</i>) Extract against Oxidative Stress-Induced Hepatotoxicity |
| title_sort | in vitro and in vivo protective effects of lentil i lens culinaris i extract against oxidative stress induced hepatotoxicity |
| topic | lentil hepatoprotective effect oxidative stress Nrf2 |
| url | https://www.mdpi.com/1420-3049/27/1/59 |
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