Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology
Despite extensive research into the toxicology of the herbicide glyphosate, there are still major unknowns regarding its effects on the human gut microbiome. We describe the effects of glyphosate and a Roundup glyphosate-based herbicide on infant gut microbiota using SHIME technology. SHIME microbio...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2022-01-01
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| Series: | Gut Microbiome |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2632289722000056/type/journal_article |
| _version_ | 1811156152431411200 |
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| author | Robin Mesnage Marta Calatayud Cindy Duysburgh Massimo Marzorati Michael N. Antoniou |
| author_facet | Robin Mesnage Marta Calatayud Cindy Duysburgh Massimo Marzorati Michael N. Antoniou |
| author_sort | Robin Mesnage |
| collection | DOAJ |
| description | Despite extensive research into the toxicology of the herbicide glyphosate, there are still major unknowns regarding its effects on the human gut microbiome. We describe the effects of glyphosate and a Roundup glyphosate-based herbicide on infant gut microbiota using SHIME technology. SHIME microbiota culture was undertaken in the presence of a concentration of 100-mg/L glyphosate and the same glyphosate equivalent concentration of Roundup. Roundup and to a lesser extent glyphosate caused an increase in fermentation activity, resulting in acidification of the microbial environment. This was also reflected by an increase in lactate and acetate production concomitant to a decrease in the levels of propionate, valerate, caproate and butyrate. Ammonium production reflecting proteolytic activities was increased by Roundup exposure. Global metabolomics revealed large-scale disturbances, including an increased abundance of long-chain polyunsaturated fatty acids. Changes in bacterial composition measured by qPCR and 16S rRNA suggested that lactobacilli had their growth stimulated as a result of microenvironment acidification. Co-treatment with the spore-based probiotic formulation MegaSporeBiotic reverted some of the changes in short-chain fatty acid levels. Altogether, our results suggest that glyphosate can exert effects on human gut microbiota. |
| first_indexed | 2024-04-10T04:46:43Z |
| format | Article |
| id | doaj.art-f36e150078384908bbce9ddbd0a34d7e |
| institution | Directory Open Access Journal |
| issn | 2632-2897 |
| language | English |
| last_indexed | 2024-04-10T04:46:43Z |
| publishDate | 2022-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Gut Microbiome |
| spelling | doaj.art-f36e150078384908bbce9ddbd0a34d7e2023-03-09T12:35:27ZengCambridge University PressGut Microbiome2632-28972022-01-01310.1017/gmb.2022.5Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technologyRobin Mesnage0https://orcid.org/0000-0003-1732-4741Marta Calatayud1Cindy Duysburgh2Massimo Marzorati3Michael N. Antoniou4Gene Expression and Therapy Group, King’s College London, Faculty of Life Sciences & Medicine, Department of Medical and Molecular Genetics, Guy’s Hospital, London, SE1 9RT, UK.ProDigest BV, Ghent, BelgiumProDigest BV, Ghent, BelgiumProDigest BV, Ghent, Belgium Center for Microbial Ecology and Technology, Faculty of Bioscience Engineering, Department of Biotechnology, Ghent University, Ghent, BelgiumGene Expression and Therapy Group, King’s College London, Faculty of Life Sciences & Medicine, Department of Medical and Molecular Genetics, Guy’s Hospital, London, SE1 9RT, UK.Despite extensive research into the toxicology of the herbicide glyphosate, there are still major unknowns regarding its effects on the human gut microbiome. We describe the effects of glyphosate and a Roundup glyphosate-based herbicide on infant gut microbiota using SHIME technology. SHIME microbiota culture was undertaken in the presence of a concentration of 100-mg/L glyphosate and the same glyphosate equivalent concentration of Roundup. Roundup and to a lesser extent glyphosate caused an increase in fermentation activity, resulting in acidification of the microbial environment. This was also reflected by an increase in lactate and acetate production concomitant to a decrease in the levels of propionate, valerate, caproate and butyrate. Ammonium production reflecting proteolytic activities was increased by Roundup exposure. Global metabolomics revealed large-scale disturbances, including an increased abundance of long-chain polyunsaturated fatty acids. Changes in bacterial composition measured by qPCR and 16S rRNA suggested that lactobacilli had their growth stimulated as a result of microenvironment acidification. Co-treatment with the spore-based probiotic formulation MegaSporeBiotic reverted some of the changes in short-chain fatty acid levels. Altogether, our results suggest that glyphosate can exert effects on human gut microbiota.https://www.cambridge.org/core/product/identifier/S2632289722000056/type/journal_articlepesticidesprobioticsmetabolomicsSHIMEglyphosategut microbiota |
| spellingShingle | Robin Mesnage Marta Calatayud Cindy Duysburgh Massimo Marzorati Michael N. Antoniou Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology Gut Microbiome pesticides probiotics metabolomics SHIME glyphosate gut microbiota |
| title | Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology |
| title_full | Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology |
| title_fullStr | Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology |
| title_full_unstemmed | Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology |
| title_short | Alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and Roundup and/or a spore-based formulation using the SHIME technology |
| title_sort | alterations in infant gut microbiome composition and metabolism after exposure to glyphosate and roundup and or a spore based formulation using the shime technology |
| topic | pesticides probiotics metabolomics SHIME glyphosate gut microbiota |
| url | https://www.cambridge.org/core/product/identifier/S2632289722000056/type/journal_article |
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