Preparation and identification of anti-breast cancer cells peptides released from yak milk casein
Yak milk casein (YMC) is the main protein in the yak milk. Peptides released from Yak milk casein (YMC) have multiple bioactivities, including anti-inflammation and immune-regulation, suggesting that these peptides might be able to inhibit cancer theoretically. However, the anti-cancer peptides from...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.997514/full |
| _version_ | 1811283409788469248 |
|---|---|
| author | Haofeng Gu Haofeng Gu Lei Liang Ziwei Zhu Xueying Mao |
| author_facet | Haofeng Gu Haofeng Gu Lei Liang Ziwei Zhu Xueying Mao |
| author_sort | Haofeng Gu |
| collection | DOAJ |
| description | Yak milk casein (YMC) is the main protein in the yak milk. Peptides released from Yak milk casein (YMC) have multiple bioactivities, including anti-inflammation and immune-regulation, suggesting that these peptides might be able to inhibit cancer theoretically. However, the anti-cancer peptides from YMC have only been sparsely studied. Breast carcinoma is the most common carcinoma in women worldwide. Thus, the paper herein was to identify yak milk casein (YMC)-derived anti-breast cancer peptides via gel filtration, reversed phase high-performance liquid chromatography (RP-HPLC) and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI MS/MS) for the first time. The inhibitory effects of the hydrolysates on the cell viabilities, cell cycles and apoptosis of breast cancer cells were evaluated with a cck8 kit and a flow cytometry. The result showed that YMC hydrolysates (YMCH) obtained by united hydrolyzation with trypsin (3 h) and alkaline protease (3 h) displayed the highest cell viability inhibition rate for MCF7 (20.74 ± 1.39%) and MDA-MB-231 (26.73 ± 2.87%) cells. Three peptides were identified in the RP-HPLC subfraction F3-4, and a nonapeptide (TPVVVPPFL) showed the most potent inhibitory effects on both cancer cells and displayed good gastrointestinal stability. TPVVVPPFL could induce G2-M cell cycle arrest in MCF7 cells and S cell arrest in MDA-MB-231 cells and induce apoptosis in both cancer cells. Moreover, in silico analysis indicated that the peptide had non-toxic and no inhibitory roles on P4502D6-enzyme. Together, this study shows that YMC is a good source of anti-breast cancer cells peptides. |
| first_indexed | 2024-04-13T02:11:00Z |
| format | Article |
| id | doaj.art-f374619d34614407a7d6737329fc8da8 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T02:11:00Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-f374619d34614407a7d6737329fc8da82022-12-22T03:07:17ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-08-01910.3389/fnut.2022.997514997514Preparation and identification of anti-breast cancer cells peptides released from yak milk caseinHaofeng Gu0Haofeng Gu1Lei Liang2Ziwei Zhu3Xueying Mao4School of Modern Agriculture and Biotechnology, Ankang University, Ankang, ChinaCollege of Food Science and Nutritional Engineering, Key Laboratory of Functional Dairy, Ministry of Education, China Agricultural University, Beijing, ChinaSchool of Modern Agriculture and Biotechnology, Ankang University, Ankang, ChinaCollege of Food Science and Nutritional Engineering, Key Laboratory of Functional Dairy, Ministry of Education, China Agricultural University, Beijing, ChinaCollege of Food Science and Nutritional Engineering, Key Laboratory of Functional Dairy, Ministry of Education, China Agricultural University, Beijing, ChinaYak milk casein (YMC) is the main protein in the yak milk. Peptides released from Yak milk casein (YMC) have multiple bioactivities, including anti-inflammation and immune-regulation, suggesting that these peptides might be able to inhibit cancer theoretically. However, the anti-cancer peptides from YMC have only been sparsely studied. Breast carcinoma is the most common carcinoma in women worldwide. Thus, the paper herein was to identify yak milk casein (YMC)-derived anti-breast cancer peptides via gel filtration, reversed phase high-performance liquid chromatography (RP-HPLC) and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI MS/MS) for the first time. The inhibitory effects of the hydrolysates on the cell viabilities, cell cycles and apoptosis of breast cancer cells were evaluated with a cck8 kit and a flow cytometry. The result showed that YMC hydrolysates (YMCH) obtained by united hydrolyzation with trypsin (3 h) and alkaline protease (3 h) displayed the highest cell viability inhibition rate for MCF7 (20.74 ± 1.39%) and MDA-MB-231 (26.73 ± 2.87%) cells. Three peptides were identified in the RP-HPLC subfraction F3-4, and a nonapeptide (TPVVVPPFL) showed the most potent inhibitory effects on both cancer cells and displayed good gastrointestinal stability. TPVVVPPFL could induce G2-M cell cycle arrest in MCF7 cells and S cell arrest in MDA-MB-231 cells and induce apoptosis in both cancer cells. Moreover, in silico analysis indicated that the peptide had non-toxic and no inhibitory roles on P4502D6-enzyme. Together, this study shows that YMC is a good source of anti-breast cancer cells peptides.https://www.frontiersin.org/articles/10.3389/fnut.2022.997514/fullhydrolysatepeptidesbreast cancer cellsyak milk caseinidentification |
| spellingShingle | Haofeng Gu Haofeng Gu Lei Liang Ziwei Zhu Xueying Mao Preparation and identification of anti-breast cancer cells peptides released from yak milk casein Frontiers in Nutrition hydrolysate peptides breast cancer cells yak milk casein identification |
| title | Preparation and identification of anti-breast cancer cells peptides released from yak milk casein |
| title_full | Preparation and identification of anti-breast cancer cells peptides released from yak milk casein |
| title_fullStr | Preparation and identification of anti-breast cancer cells peptides released from yak milk casein |
| title_full_unstemmed | Preparation and identification of anti-breast cancer cells peptides released from yak milk casein |
| title_short | Preparation and identification of anti-breast cancer cells peptides released from yak milk casein |
| title_sort | preparation and identification of anti breast cancer cells peptides released from yak milk casein |
| topic | hydrolysate peptides breast cancer cells yak milk casein identification |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.997514/full |
| work_keys_str_mv | AT haofenggu preparationandidentificationofantibreastcancercellspeptidesreleasedfromyakmilkcasein AT haofenggu preparationandidentificationofantibreastcancercellspeptidesreleasedfromyakmilkcasein AT leiliang preparationandidentificationofantibreastcancercellspeptidesreleasedfromyakmilkcasein AT ziweizhu preparationandidentificationofantibreastcancercellspeptidesreleasedfromyakmilkcasein AT xueyingmao preparationandidentificationofantibreastcancercellspeptidesreleasedfromyakmilkcasein |