New insights into phenotype and genotype relationships in Neospora caninum
The successful isolation of four new Neospora caninum strains from different regions and with different backgrounds (obtained from an abortion storm or congenitally infected and asymptomatic calves) allowed us previously to characterize natural isolates, finding differences in phenotype and microsat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1214971/full |
| _version_ | 1828717349662883840 |
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| author | Andres Cabrera Andres Cabrera Luisa Berná Luisa Berná Lucía López Paula Faral-Tello Ana Paula Arevalo Martina Crispo Maria E. Francia Maria E. Francia Carlos Robello Carlos Robello |
| author_facet | Andres Cabrera Andres Cabrera Luisa Berná Luisa Berná Lucía López Paula Faral-Tello Ana Paula Arevalo Martina Crispo Maria E. Francia Maria E. Francia Carlos Robello Carlos Robello |
| author_sort | Andres Cabrera |
| collection | DOAJ |
| description | The successful isolation of four new Neospora caninum strains from different regions and with different backgrounds (obtained from an abortion storm or congenitally infected and asymptomatic calves) allowed us previously to characterize natural isolates, finding differences in phenotype and microsatellites. Given the variability observed, we wondered in this work whether these differences had consequences in virulence, invasion and vertical transmission using cell cultures and murine neosporosis models. In addition, we performed the genomic analysis and SNP comparative studies of the NcURU isolates. The results obtained in this work allowed us to establish that NcURU isolates are of low virulence and have unique phenotypic characteristics. Likewise, sequencing their genomes has allowed us to delve into the genetic singularities underlying these phenotypes, as well as the common mutated genes. This work opens a new perspective for diagnostic purposes and formulating possible vaccines based on attenuated strains. |
| first_indexed | 2024-03-12T14:26:50Z |
| format | Article |
| id | doaj.art-f37504428db4426ebbc9147b5bae8e25 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-12T14:26:50Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-f37504428db4426ebbc9147b5bae8e252023-08-18T05:25:32ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-08-011010.3389/fvets.2023.12149711214971New insights into phenotype and genotype relationships in Neospora caninumAndres Cabrera0Andres Cabrera1Luisa Berná2Luisa Berná3Lucía López4Paula Faral-Tello5Ana Paula Arevalo6Martina Crispo7Maria E. Francia8Maria E. Francia9Carlos Robello10Carlos Robello11Laboratorio de Interacciones Hospedero-Patógeno -UBM, Institut Pasteur de Montevideo, Montevideo, UruguayDepartamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, UruguayLaboratorio de Interacciones Hospedero-Patógeno -UBM, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratorio de Genómica Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayLaboratorio de Interacciones Hospedero-Patógeno -UBM, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratorio de Interacciones Hospedero-Patógeno -UBM, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratory Animals Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratory Animals Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, UruguayDepartamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, UruguayLaboratory of Apicomplexan Biology, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratorio de Interacciones Hospedero-Patógeno -UBM, Institut Pasteur de Montevideo, Montevideo, UruguayDepartamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, UruguayThe successful isolation of four new Neospora caninum strains from different regions and with different backgrounds (obtained from an abortion storm or congenitally infected and asymptomatic calves) allowed us previously to characterize natural isolates, finding differences in phenotype and microsatellites. Given the variability observed, we wondered in this work whether these differences had consequences in virulence, invasion and vertical transmission using cell cultures and murine neosporosis models. In addition, we performed the genomic analysis and SNP comparative studies of the NcURU isolates. The results obtained in this work allowed us to establish that NcURU isolates are of low virulence and have unique phenotypic characteristics. Likewise, sequencing their genomes has allowed us to delve into the genetic singularities underlying these phenotypes, as well as the common mutated genes. This work opens a new perspective for diagnostic purposes and formulating possible vaccines based on attenuated strains.https://www.frontiersin.org/articles/10.3389/fvets.2023.1214971/fullNeospora caninumvirulencechronicityvertical transmissionabortionanimal health |
| spellingShingle | Andres Cabrera Andres Cabrera Luisa Berná Luisa Berná Lucía López Paula Faral-Tello Ana Paula Arevalo Martina Crispo Maria E. Francia Maria E. Francia Carlos Robello Carlos Robello New insights into phenotype and genotype relationships in Neospora caninum Frontiers in Veterinary Science Neospora caninum virulence chronicity vertical transmission abortion animal health |
| title | New insights into phenotype and genotype relationships in Neospora caninum |
| title_full | New insights into phenotype and genotype relationships in Neospora caninum |
| title_fullStr | New insights into phenotype and genotype relationships in Neospora caninum |
| title_full_unstemmed | New insights into phenotype and genotype relationships in Neospora caninum |
| title_short | New insights into phenotype and genotype relationships in Neospora caninum |
| title_sort | new insights into phenotype and genotype relationships in neospora caninum |
| topic | Neospora caninum virulence chronicity vertical transmission abortion animal health |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1214971/full |
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