Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters
Interleukin (IL)-1α, IL-1β, IL-6, IL-8 and tumor necrosis factor (TNF)α contribute to inflammation in chronic obstructive pulmonary disease (COPD). We wanted to investigate their interrelations and association with disease severity, as well as to combine them with other inflammation-associated bioma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/12/1029 |
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| author | Iva Hlapčić Daniela Belamarić Martina Bosnar Domagoj Kifer Andrea Vukić Dugac Lada Rumora |
| author_facet | Iva Hlapčić Daniela Belamarić Martina Bosnar Domagoj Kifer Andrea Vukić Dugac Lada Rumora |
| author_sort | Iva Hlapčić |
| collection | DOAJ |
| description | Interleukin (IL)-1α, IL-1β, IL-6, IL-8 and tumor necrosis factor (TNF)α contribute to inflammation in chronic obstructive pulmonary disease (COPD). We wanted to investigate their interrelations and association with disease severity, as well as to combine them with other inflammation-associated biomarkers and evaluate their predictive value and potential in identifying various patterns of systemic inflammation. One hundred and nine patients with stable COPD and 95 age- and sex-matched controls were enrolled in the study. Cytokines’ concentrations were determined in plasma samples by antibody-based multiplex immunosorbent assay kits. Investigated cytokines were increased in COPD patients but were not associated with disease or symptoms severity. IL-1β, IL-6 and TNFα showed the best discriminative values regarding ongoing inflammation in COPD. Inflammatory patterns were observed in COPD patients when cytokines, C-reactive protein (CRP), fibrinogen (Fbg), extracellular adenosine triphosphate (eATP), extracellular heat shock protein 70 (eHsp70) and clinical data were included in cluster analysis. IL-1β, eATP and eHsp70 combined correctly classified 91% of cases. Therefore, due to the heterogeneity of COPD, its assessment could be improved by combination of biomarkers. Models including IL-1β, eATP and eHsp70 might identify COPD patients, while IL-1β, IL-6 and TNFα combined with CRP, Fbg, eATP and eHsp70 might be informative regarding various COPD clinical subgroups. |
| first_indexed | 2024-03-10T14:25:47Z |
| format | Article |
| id | doaj.art-f379b6a7ad0341c7bcdc2058473edee1 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T14:25:47Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-f379b6a7ad0341c7bcdc2058473edee12023-11-20T23:01:12ZengMDPI AGDiagnostics2075-44182020-11-011012102910.3390/diagnostics10121029Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and ClustersIva Hlapčić0Daniela Belamarić1Martina Bosnar2Domagoj Kifer3Andrea Vukić Dugac4Lada Rumora5Department of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, CroatiaFidelta Ltd., 10000 Zagreb, CroatiaFidelta Ltd., 10000 Zagreb, CroatiaDepartment of Biophysics, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, CroatiaClinical Department for Lung Diseases Jordanovac, University Hospital Centre Zagreb, 10000 Zagreb, CroatiaDepartment of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, CroatiaInterleukin (IL)-1α, IL-1β, IL-6, IL-8 and tumor necrosis factor (TNF)α contribute to inflammation in chronic obstructive pulmonary disease (COPD). We wanted to investigate their interrelations and association with disease severity, as well as to combine them with other inflammation-associated biomarkers and evaluate their predictive value and potential in identifying various patterns of systemic inflammation. One hundred and nine patients with stable COPD and 95 age- and sex-matched controls were enrolled in the study. Cytokines’ concentrations were determined in plasma samples by antibody-based multiplex immunosorbent assay kits. Investigated cytokines were increased in COPD patients but were not associated with disease or symptoms severity. IL-1β, IL-6 and TNFα showed the best discriminative values regarding ongoing inflammation in COPD. Inflammatory patterns were observed in COPD patients when cytokines, C-reactive protein (CRP), fibrinogen (Fbg), extracellular adenosine triphosphate (eATP), extracellular heat shock protein 70 (eHsp70) and clinical data were included in cluster analysis. IL-1β, eATP and eHsp70 combined correctly classified 91% of cases. Therefore, due to the heterogeneity of COPD, its assessment could be improved by combination of biomarkers. Models including IL-1β, eATP and eHsp70 might identify COPD patients, while IL-1β, IL-6 and TNFα combined with CRP, Fbg, eATP and eHsp70 might be informative regarding various COPD clinical subgroups.https://www.mdpi.com/2075-4418/10/12/1029chronic obstructive pulmonary diseasecytokinessystemic inflammationclustersadenosine triphosphateheat shock protein 70 |
| spellingShingle | Iva Hlapčić Daniela Belamarić Martina Bosnar Domagoj Kifer Andrea Vukić Dugac Lada Rumora Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters Diagnostics chronic obstructive pulmonary disease cytokines systemic inflammation clusters adenosine triphosphate heat shock protein 70 |
| title | Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters |
| title_full | Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters |
| title_fullStr | Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters |
| title_full_unstemmed | Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters |
| title_short | Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters |
| title_sort | combination of systemic inflammatory biomarkers in assessment of chronic obstructive pulmonary disease diagnostic performance and identification of networks and clusters |
| topic | chronic obstructive pulmonary disease cytokines systemic inflammation clusters adenosine triphosphate heat shock protein 70 |
| url | https://www.mdpi.com/2075-4418/10/12/1029 |
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