OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES
Currently, diabetes ranks third in relation to medical and social significance after cardiovascular diseases and cancer and is the leading cause of blindness; it greatly increases the risk of myocardial infarction, coronary heart disease, nephropathy and hypertension in patients with this disorder;...
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| Format: | Article |
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Siberian State Medical University (Tomsk)
2014-06-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/69 |
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| author | Ye. V. Shakhristova Ye. A. Stepovaya V. V. Ivanov O. L. Nosareva N. V. Ryazantseva V. V. Novitsky |
| author_facet | Ye. V. Shakhristova Ye. A. Stepovaya V. V. Ivanov O. L. Nosareva N. V. Ryazantseva V. V. Novitsky |
| author_sort | Ye. V. Shakhristova |
| collection | DOAJ |
| description | Currently, diabetes ranks third in relation to medical and social significance after cardiovascular diseases and cancer and is the leading cause of blindness; it greatly increases the risk of myocardial infarction, coronary heart disease, nephropathy and hypertension in patients with this disorder; therefore clinical and experimental studies aimed at investigation of diabetes emergence and development mechanisms are urgent.The aim of the study was to investigate the status of oxidative modification of proteins and glutathionedependent antioxidant defense system in adipocytes of rats with alloxan diabetes under conditions of oxidative stress.Material and methods. Development of type 1 diabetes was induced in rats by alloxan administration (90 mg/kg of body mass). Adipocytes were obtained from epididymal adipose tissue of rats. The level of carbonyl derivatives of proteins, oxidized tryptophan, bityrosine, general, reduced, oxygenated and protein-bound glutathione, as well as glutathione peroxidase activity in adipocytes of rats was determined.Results. In adipocytes of rats with alloxan diabetes, concentration of carbonyl derivatives of proteins, bityrosine and oxidized tryptophan increased on the background of redox-potential of glutathione system and glutathione peroxidase activity decrease.Conclusion. The obtained data indicate the activation of free-radical oxidation of proteins and reduction of antioxidant defense under conditions of oxidative stress in the adipose tissue of rats with alloxan diabetes; this process plays an important role in pathogenesis of diabetes and its complications development. |
| first_indexed | 2024-04-10T01:08:12Z |
| format | Article |
| id | doaj.art-f37d718ad4c448f0b4c2d840dbcbf23a |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:08:12Z |
| publishDate | 2014-06-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-f37d718ad4c448f0b4c2d840dbcbf23a2023-03-13T09:58:20ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842014-06-01133849010.20538/1682-0363-2014-3-84-9067OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETESYe. V. Shakhristova0Ye. A. Stepovaya1V. V. Ivanov2O. L. Nosareva3N. V. Ryazantseva4V. V. Novitsky5Сибирский государственный медицинский институт, ТомскСибирский государственный медицинский институт, ТомскСибирский государственный медицинский институт, ТомскСибирский государственный медицинский институт, ТомскСибирский государственный медицинский институт, ТомскСибирский государственный медицинский институт, ТомскCurrently, diabetes ranks third in relation to medical and social significance after cardiovascular diseases and cancer and is the leading cause of blindness; it greatly increases the risk of myocardial infarction, coronary heart disease, nephropathy and hypertension in patients with this disorder; therefore clinical and experimental studies aimed at investigation of diabetes emergence and development mechanisms are urgent.The aim of the study was to investigate the status of oxidative modification of proteins and glutathionedependent antioxidant defense system in adipocytes of rats with alloxan diabetes under conditions of oxidative stress.Material and methods. Development of type 1 diabetes was induced in rats by alloxan administration (90 mg/kg of body mass). Adipocytes were obtained from epididymal adipose tissue of rats. The level of carbonyl derivatives of proteins, oxidized tryptophan, bityrosine, general, reduced, oxygenated and protein-bound glutathione, as well as glutathione peroxidase activity in adipocytes of rats was determined.Results. In adipocytes of rats with alloxan diabetes, concentration of carbonyl derivatives of proteins, bityrosine and oxidized tryptophan increased on the background of redox-potential of glutathione system and glutathione peroxidase activity decrease.Conclusion. The obtained data indicate the activation of free-radical oxidation of proteins and reduction of antioxidant defense under conditions of oxidative stress in the adipose tissue of rats with alloxan diabetes; this process plays an important role in pathogenesis of diabetes and its complications development.https://bulletin.ssmu.ru/jour/article/view/69сахарный диабетадипоцитыокислительный стресскарбонильные производные белковокисленный триптофанбитирозинглутатион |
| spellingShingle | Ye. V. Shakhristova Ye. A. Stepovaya V. V. Ivanov O. L. Nosareva N. V. Ryazantseva V. V. Novitsky OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES Бюллетень сибирской медицины сахарный диабет адипоциты окислительный стресс карбонильные производные белков окисленный триптофан битирозин глутатион |
| title | OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES |
| title_full | OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES |
| title_fullStr | OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES |
| title_full_unstemmed | OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES |
| title_short | OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES |
| title_sort | oxidative modification of proteins and glutathione system in adipocytes under diabetes |
| topic | сахарный диабет адипоциты окислительный стресс карбонильные производные белков окисленный триптофан битирозин глутатион |
| url | https://bulletin.ssmu.ru/jour/article/view/69 |
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