Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells
In the present study, we evaluated the effects of biological factors, lifestyle factors, and environmental conditions on the induction of DNA damage in exfoliated cells of the oral mucosa. Age, sex, medication use, and environmental conditions were analyzed in individuals residing in the cities of C...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-06-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019317438 |
| _version_ | 1818849049463226368 |
|---|---|
| author | Deborah Navit de Carvalho Cavalcante Bruno do Amaral Crispim Beatriz Barufatti Grisolia Lucilene Finoto Viana Nayara Halimy Maran Julio César Jut Solórzano Kelly Mari Pires de Oliveira Alexeia Barufatti |
| author_facet | Deborah Navit de Carvalho Cavalcante Bruno do Amaral Crispim Beatriz Barufatti Grisolia Lucilene Finoto Viana Nayara Halimy Maran Julio César Jut Solórzano Kelly Mari Pires de Oliveira Alexeia Barufatti |
| author_sort | Deborah Navit de Carvalho Cavalcante |
| collection | DOAJ |
| description | In the present study, we evaluated the effects of biological factors, lifestyle factors, and environmental conditions on the induction of DNA damage in exfoliated cells of the oral mucosa. Age, sex, medication use, and environmental conditions were analyzed in individuals residing in the cities of Caarapó and Itaporã. The individuals were assessed by a questionnaire, and oral mucosa cells were collected and subjected to mutagenicity analysis. We observed no statistical differences in DNA damage related to sex. However, the mutagenic effect was found to be proportional to age, with higher frequencies of DNA damage observed in individuals between the ages of 46 and 65 years. In addition, higher frequencies of DNA damage were found in individuals who continuously used medication and for prolonged periods, and greater DNA damage was observed in individuals who used antihypertensive drugs than those who took antidepressants. In terms of environmental conditions, Caarapó residents had a significantly higher frequency of DNA damage than that of residents from Itaporã. Based on the analysis of land use and occupation, this result can be attributed to the smaller fraction of forest fragments and the higher proportion of buildings in Caarapó than Itaporã. We concluded that age, continued medication use, and environmental conditions can lead to greater DNA damage. |
| first_indexed | 2024-12-19T06:27:03Z |
| format | Article |
| id | doaj.art-f37f9597d8124b28804dd3093e5f13a4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T06:27:03Z |
| publishDate | 2019-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f37f9597d8124b28804dd3093e5f13a42022-12-21T20:32:31ZengElsevierHeliyon2405-84402019-06-0156e01840Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cellsDeborah Navit de Carvalho Cavalcante0Bruno do Amaral Crispim1Beatriz Barufatti Grisolia2Lucilene Finoto Viana3Nayara Halimy Maran4Julio César Jut Solórzano5Kelly Mari Pires de Oliveira6Alexeia Barufatti7Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, BrazilFaculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, BrazilUniversidade Estadual de Maringá, Av. Colombo, 5790 – Jd. Universitário, CEP 87020-900, Maringá, PR, BrazilUniversidade Estadual do Mato Grosso do Sul, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 351, CEP 79804-970, Dourados, MS, BrazilFaculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, BrazilUniversidade Estadual do Mato Grosso do Sul, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 351, CEP 79804-970, Dourados, MS, BrazilFaculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, BrazilFaculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, Brazil; Corresponding author.In the present study, we evaluated the effects of biological factors, lifestyle factors, and environmental conditions on the induction of DNA damage in exfoliated cells of the oral mucosa. Age, sex, medication use, and environmental conditions were analyzed in individuals residing in the cities of Caarapó and Itaporã. The individuals were assessed by a questionnaire, and oral mucosa cells were collected and subjected to mutagenicity analysis. We observed no statistical differences in DNA damage related to sex. However, the mutagenic effect was found to be proportional to age, with higher frequencies of DNA damage observed in individuals between the ages of 46 and 65 years. In addition, higher frequencies of DNA damage were found in individuals who continuously used medication and for prolonged periods, and greater DNA damage was observed in individuals who used antihypertensive drugs than those who took antidepressants. In terms of environmental conditions, Caarapó residents had a significantly higher frequency of DNA damage than that of residents from Itaporã. Based on the analysis of land use and occupation, this result can be attributed to the smaller fraction of forest fragments and the higher proportion of buildings in Caarapó than Itaporã. We concluded that age, continued medication use, and environmental conditions can lead to greater DNA damage.http://www.sciencedirect.com/science/article/pii/S2405844019317438KaryorrhexisLand use and coverageMutagenesisMicronucleusPharmaceutical drugkGenetics |
| spellingShingle | Deborah Navit de Carvalho Cavalcante Bruno do Amaral Crispim Beatriz Barufatti Grisolia Lucilene Finoto Viana Nayara Halimy Maran Julio César Jut Solórzano Kelly Mari Pires de Oliveira Alexeia Barufatti Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells Heliyon Karyorrhexis Land use and coverage Mutagenesis Micronucleus Pharmaceutical drugk Genetics |
| title | Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells |
| title_full | Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells |
| title_fullStr | Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells |
| title_full_unstemmed | Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells |
| title_short | Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells |
| title_sort | effects of age sex medication and environmental conditions on genetic alterations in oral mucosa cells |
| topic | Karyorrhexis Land use and coverage Mutagenesis Micronucleus Pharmaceutical drugk Genetics |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019317438 |
| work_keys_str_mv | AT deborahnavitdecarvalhocavalcante effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT brunodoamaralcrispim effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT beatrizbarufattigrisolia effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT lucilenefinotoviana effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT nayarahalimymaran effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT juliocesarjutsolorzano effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT kellymaripiresdeoliveira effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells AT alexeiabarufatti effectsofagesexmedicationandenvironmentalconditionsongeneticalterationsinoralmucosacells |