Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae)
The whitefly, Bemisia tabaci, an insect of the order Hemiptera which attacks more than 600 species of plants, is one of the most important agricultural pests around the world. The insecticidal Cry proteins from Bacillus thuringiensis (Bt) are useful biological pesticides, and some are toxic to He...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2019-12-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2019.131259 |
| _version_ | 1818320590914715648 |
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| author | Eneida Torres Cabra Javier Adolfo Hernandez Fernandez |
| author_facet | Eneida Torres Cabra Javier Adolfo Hernandez Fernandez |
| author_sort | Eneida Torres Cabra |
| collection | DOAJ |
| description | The whitefly, Bemisia tabaci, an insect of the order Hemiptera which attacks more than
600 species of plants, is one of the most important agricultural pests around the world. The
insecticidal Cry proteins from Bacillus thuringiensis (Bt) are useful biological pesticides,
and some are toxic to Hemipteran insects. In this study, Colombian native isolates of Bt
were functionally characterized at molecular and biological levels. The strains contained
between one and five different crystal shapes: round, triangular, amorphous, bipyramidal
and squared. The strains presented between three to seven bands of proteins in their electrophoretic
pattern that were organized into six groups according to their possible biological
activity on insect pests. Cry1Aa, cry1Ab, cry1Ac, cry1B and cry1C genes were identified
for PCR in the different Bt isolates. Bioassays were performed on tomato leaves whose surface
was spread with 3 μg · ml−1 crude extract of Bt toxins. Second instar larvae of whitefly,
which were placed on top of leaves and exposed to the toxins for 7 days, exhibited mortalities
from 18 to 69%. The lethal concentration 50 (LC50) of ZBUJTL39, Bt kurstaki HD1 and
ZCUJTL9 strains were 1.83, 1.85 and 2.16 μg · ml−1, respectively (p < 0.05). These results
show that the native Bt strain ZBUJTL39, which contained the genes cry1Aa, cry1Ab, cryCa
and cryBa could eventually be used for the development of an integrated management program
together with other tools for the control of B. tabaci. |
| first_indexed | 2024-12-13T10:27:26Z |
| format | Article |
| id | doaj.art-f382aca35ae444b48d8d57b84784b089 |
| institution | Directory Open Access Journal |
| issn | 1899-007X 1899-007X |
| language | English |
| last_indexed | 2024-12-13T10:27:26Z |
| publishDate | 2019-12-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-f382aca35ae444b48d8d57b84784b0892022-12-21T23:51:00ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2019-12-0159450351110.24425/jppr.2019.131259Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae)Eneida Torres Cabra0Javier Adolfo Hernandez Fernandez1Department of Sciences and Agricultural Sciences, Faculty of Science of Agricultural, Juan de Castellanos University Foundation, Tunja, ColombiaDepartment of Natural Science and Environmental, Faculty of Science and Engineering, Jorge Tadeo Lozano University, Bogotá, ColombiaThe whitefly, Bemisia tabaci, an insect of the order Hemiptera which attacks more than 600 species of plants, is one of the most important agricultural pests around the world. The insecticidal Cry proteins from Bacillus thuringiensis (Bt) are useful biological pesticides, and some are toxic to Hemipteran insects. In this study, Colombian native isolates of Bt were functionally characterized at molecular and biological levels. The strains contained between one and five different crystal shapes: round, triangular, amorphous, bipyramidal and squared. The strains presented between three to seven bands of proteins in their electrophoretic pattern that were organized into six groups according to their possible biological activity on insect pests. Cry1Aa, cry1Ab, cry1Ac, cry1B and cry1C genes were identified for PCR in the different Bt isolates. Bioassays were performed on tomato leaves whose surface was spread with 3 μg · ml−1 crude extract of Bt toxins. Second instar larvae of whitefly, which were placed on top of leaves and exposed to the toxins for 7 days, exhibited mortalities from 18 to 69%. The lethal concentration 50 (LC50) of ZBUJTL39, Bt kurstaki HD1 and ZCUJTL9 strains were 1.83, 1.85 and 2.16 μg · ml−1, respectively (p < 0.05). These results show that the native Bt strain ZBUJTL39, which contained the genes cry1Aa, cry1Ab, cryCa and cryBa could eventually be used for the development of an integrated management program together with other tools for the control of B. tabaci.https://doi.org/10.24425/jppr.2019.131259biological controlgenes cryinsect pestmolecular characterization |
| spellingShingle | Eneida Torres Cabra Javier Adolfo Hernandez Fernandez Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae) Journal of Plant Protection Research biological control genes cry insect pest molecular characterization |
| title | Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae) |
| title_full | Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae) |
| title_fullStr | Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae) |
| title_full_unstemmed | Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae) |
| title_short | Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae) |
| title_sort | larvicidal activity of bacillus thuringiensis colombian native strains against bemisia tabaci hemiptera aleyrodidae |
| topic | biological control genes cry insect pest molecular characterization |
| url | https://doi.org/10.24425/jppr.2019.131259 |
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