Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting
Summary: Muscle stem cells (MuSCs) are the building blocks for regenerating skeletal muscle after trauma. If we intend to maximize the therapeutic potential of MuSCs, we must further study their molecular and functional properties. Here, we present a protocol for the isolation of mouse MuSCs via a t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723006238 |
| _version_ | 1797652564833992704 |
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| author | Gabriel Elizalde Alma Zuniga Munoz Albert E. Almada |
| author_facet | Gabriel Elizalde Alma Zuniga Munoz Albert E. Almada |
| author_sort | Gabriel Elizalde |
| collection | DOAJ |
| description | Summary: Muscle stem cells (MuSCs) are the building blocks for regenerating skeletal muscle after trauma. If we intend to maximize the therapeutic potential of MuSCs, we must further study their molecular and functional properties. Here, we present a protocol for the isolation of mouse MuSCs via a two-step enzymatic and mechanical dissociation of skeletal muscle coupled with fluorescence-activated cell sorting (FACS). FACS-isolated MuSCs can be used for various downstream applications including cell culture, cell transduction, immunofluorescence, and gene expression assays.For complete details on the use and execution of this protocol, please refer to Almada et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-11T16:31:47Z |
| format | Article |
| id | doaj.art-f38644e877424e8d8a6705defafffb71 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-11T16:31:47Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f38644e877424e8d8a6705defafffb712023-10-24T04:07:01ZengElsevierSTAR Protocols2666-16672023-12-0144102656Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sortingGabriel Elizalde0Alma Zuniga Munoz1Albert E. Almada2Department of Orthopaedic Surgery, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USADepartment of Orthopaedic Surgery, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USADepartment of Orthopaedic Surgery, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USA; Corresponding authorSummary: Muscle stem cells (MuSCs) are the building blocks for regenerating skeletal muscle after trauma. If we intend to maximize the therapeutic potential of MuSCs, we must further study their molecular and functional properties. Here, we present a protocol for the isolation of mouse MuSCs via a two-step enzymatic and mechanical dissociation of skeletal muscle coupled with fluorescence-activated cell sorting (FACS). FACS-isolated MuSCs can be used for various downstream applications including cell culture, cell transduction, immunofluorescence, and gene expression assays.For complete details on the use and execution of this protocol, please refer to Almada et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723006238Cell isolationFlow CytometryGene ExpressionAntibodyStem CellsCell Differentiation |
| spellingShingle | Gabriel Elizalde Alma Zuniga Munoz Albert E. Almada Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting STAR Protocols Cell isolation Flow Cytometry Gene Expression Antibody Stem Cells Cell Differentiation |
| title | Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting |
| title_full | Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting |
| title_fullStr | Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting |
| title_full_unstemmed | Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting |
| title_short | Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting |
| title_sort | protocol for the isolation of mouse muscle stem cells using fluorescence activated cell sorting |
| topic | Cell isolation Flow Cytometry Gene Expression Antibody Stem Cells Cell Differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723006238 |
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