Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway
Sea buckthorn (<i>Hippophaë rhamnoides</i> L.), as one of the Elaeagnaceae family, has the significant function of anti-tumor, anti-inflammation, anti-oxidation, and other physiological activities. High hydrostatic pressure (HHP) extraction has the advantages of being easy and efficient,...
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MDPI AG
2024-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/4/560 |
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| author | Zhuomin Yan Xiaoping Feng Xinian Li Zhenpeng Gao Zhouli Wang Guangxu Ren Fangyu Long |
| author_facet | Zhuomin Yan Xiaoping Feng Xinian Li Zhenpeng Gao Zhouli Wang Guangxu Ren Fangyu Long |
| author_sort | Zhuomin Yan |
| collection | DOAJ |
| description | Sea buckthorn (<i>Hippophaë rhamnoides</i> L.), as one of the Elaeagnaceae family, has the significant function of anti-tumor, anti-inflammation, anti-oxidation, and other physiological activities. High hydrostatic pressure (HHP) extraction has the advantages of being easy and efficient, while maintaining biological activity. In this study, sea buckthorn flavonoid (SBF) was extracted with HHP and purified sea buckthorn flavonoid (PSBF) was isolated by AB-8 macroporous resin column. HPLC analysis was used to quantified them. In addition, the effect of anti-allergy in RBL-2H3 cells by SBF, PSBF, and their flavonoid compounds was evaluated. The results demonstrate the conditions for obtaining the maximum flavonoid amount of SBF: 415 MPa for 10 min, 72% ethanol concentration, and a liquid to solid ratio of 40 mL/g, which increased the purity from 1.46% to 13.26%. Both SBF and PSBF included rutin, quercitrin, quercetin, isorhamnetin, and kaempferol. In addition, quercitrin, kaempferol, and SBF could regulate Th1/Th2 cytokine balance. Moreover, extracellular Ca<sup>2+</sup> influx was reduced by quercitrin and PSBF. Furthermore, rutin, quercetin, iso-rhamnetin, and SBF could also inhibit P-p38 and P-JNK expression, thereby suppressing the phosphorylation of the MAPK signaling pathways. Overall, SBF is effective for relieving food allergy and might be a promising anti-allergic therapeutic agent. |
| first_indexed | 2024-03-07T22:33:07Z |
| format | Article |
| id | doaj.art-f38b685937dd41e8a94274c1fa9c82d7 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-07T22:33:07Z |
| publishDate | 2024-02-01 |
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| series | Foods |
| spelling | doaj.art-f38b685937dd41e8a94274c1fa9c82d72024-02-23T15:16:24ZengMDPI AGFoods2304-81582024-02-0113456010.3390/foods13040560Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling PathwayZhuomin Yan0Xiaoping Feng1Xinian Li2Zhenpeng Gao3Zhouli Wang4Guangxu Ren5Fangyu Long6College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Beijing 100081, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaSea buckthorn (<i>Hippophaë rhamnoides</i> L.), as one of the Elaeagnaceae family, has the significant function of anti-tumor, anti-inflammation, anti-oxidation, and other physiological activities. High hydrostatic pressure (HHP) extraction has the advantages of being easy and efficient, while maintaining biological activity. In this study, sea buckthorn flavonoid (SBF) was extracted with HHP and purified sea buckthorn flavonoid (PSBF) was isolated by AB-8 macroporous resin column. HPLC analysis was used to quantified them. In addition, the effect of anti-allergy in RBL-2H3 cells by SBF, PSBF, and their flavonoid compounds was evaluated. The results demonstrate the conditions for obtaining the maximum flavonoid amount of SBF: 415 MPa for 10 min, 72% ethanol concentration, and a liquid to solid ratio of 40 mL/g, which increased the purity from 1.46% to 13.26%. Both SBF and PSBF included rutin, quercitrin, quercetin, isorhamnetin, and kaempferol. In addition, quercitrin, kaempferol, and SBF could regulate Th1/Th2 cytokine balance. Moreover, extracellular Ca<sup>2+</sup> influx was reduced by quercitrin and PSBF. Furthermore, rutin, quercetin, iso-rhamnetin, and SBF could also inhibit P-p38 and P-JNK expression, thereby suppressing the phosphorylation of the MAPK signaling pathways. Overall, SBF is effective for relieving food allergy and might be a promising anti-allergic therapeutic agent.https://www.mdpi.com/2304-8158/13/4/560allergysea buckthorn flavonoidHHPRBL-2H3MAPK |
| spellingShingle | Zhuomin Yan Xiaoping Feng Xinian Li Zhenpeng Gao Zhouli Wang Guangxu Ren Fangyu Long Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway Foods allergy sea buckthorn flavonoid HHP RBL-2H3 MAPK |
| title | Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_full | Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_fullStr | Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_full_unstemmed | Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_short | Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_sort | sea buckthorn flavonoid extracted by high hydrostatic pressure inhibited ige stimulated mast cell activation through the mitogen activated protein kinase signaling pathway |
| topic | allergy sea buckthorn flavonoid HHP RBL-2H3 MAPK |
| url | https://www.mdpi.com/2304-8158/13/4/560 |
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