Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster
Perilipins are evolutionarily conserved from Drosophila to humans, the lipid storage droplet 1 (Lsd1) is a Drosophila homolog of human perilipin 1. The function of Lsd1 as a regulator of lipolysis in Drosophila has been demonstrated, as the Lsd1 mutant causes an increase of lipid droplet size. Howev...
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MDPI AG
2016-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/17/5/648 |
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| author | Tran Thanh Men Tran Duy Binh Masamitsu Yamaguchi Nguyen Tien Huy Kaeko Kamei |
| author_facet | Tran Thanh Men Tran Duy Binh Masamitsu Yamaguchi Nguyen Tien Huy Kaeko Kamei |
| author_sort | Tran Thanh Men |
| collection | DOAJ |
| description | Perilipins are evolutionarily conserved from Drosophila to humans, the lipid storage droplet 1 (Lsd1) is a Drosophila homolog of human perilipin 1. The function of Lsd1 as a regulator of lipolysis in Drosophila has been demonstrated, as the Lsd1 mutant causes an increase of lipid droplet size. However, the functions of this gene during development are still under investigation. In order to determine the function of Lsd1 during development, Lsd1 was knocked down in Drosophila using the GAL4-UAS system. Selective knockdown of Lsd1 in the dorsal wing disc caused an atrophied wing phenotype. The generation of reactive oxygen species in the wing pouch compartment of the Lsd1-knockdown flies was significantly higher than in the control. Immunostaining with caspase-3 antibody revealed a greater number of apoptotic cells in Lsd1-knockdown wing discs than in the control. Cell death by autophagy was also induced in the knockdown flies. Moreover, cells deprived of Lsd1 showed mitochondrial expansion and decreased ATP levels. These results strongly suggest that knockdown of Lsd1 induces mitochondrial stress and the production of reactive oxygen species that result in cell death, via apoptosis and the autophagy pathway. These results highlight the roles of Drosophila Lsd1 during wing development. |
| first_indexed | 2024-04-13T01:44:46Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T01:44:46Z |
| publishDate | 2016-04-01 |
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| spelling | doaj.art-f38cb21508f447c8a05971f74754e9902022-12-22T03:08:05ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-04-0117564810.3390/ijms17050648ijms17050648Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogasterTran Thanh Men0Tran Duy Binh1Masamitsu Yamaguchi2Nguyen Tien Huy3Kaeko Kamei4Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, JapanDepartment of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, JapanDepartment of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585, JapanDepartment of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, JapanDepartment of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, JapanPerilipins are evolutionarily conserved from Drosophila to humans, the lipid storage droplet 1 (Lsd1) is a Drosophila homolog of human perilipin 1. The function of Lsd1 as a regulator of lipolysis in Drosophila has been demonstrated, as the Lsd1 mutant causes an increase of lipid droplet size. However, the functions of this gene during development are still under investigation. In order to determine the function of Lsd1 during development, Lsd1 was knocked down in Drosophila using the GAL4-UAS system. Selective knockdown of Lsd1 in the dorsal wing disc caused an atrophied wing phenotype. The generation of reactive oxygen species in the wing pouch compartment of the Lsd1-knockdown flies was significantly higher than in the control. Immunostaining with caspase-3 antibody revealed a greater number of apoptotic cells in Lsd1-knockdown wing discs than in the control. Cell death by autophagy was also induced in the knockdown flies. Moreover, cells deprived of Lsd1 showed mitochondrial expansion and decreased ATP levels. These results strongly suggest that knockdown of Lsd1 induces mitochondrial stress and the production of reactive oxygen species that result in cell death, via apoptosis and the autophagy pathway. These results highlight the roles of Drosophila Lsd1 during wing development.http://www.mdpi.com/1422-0067/17/5/648Drosophilalipid storage droplet 1mitochondrial stressperilipinwing development |
| spellingShingle | Tran Thanh Men Tran Duy Binh Masamitsu Yamaguchi Nguyen Tien Huy Kaeko Kamei Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster International Journal of Molecular Sciences Drosophila lipid storage droplet 1 mitochondrial stress perilipin wing development |
| title | Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster |
| title_full | Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster |
| title_fullStr | Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster |
| title_full_unstemmed | Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster |
| title_short | Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster |
| title_sort | function of lipid storage droplet 1 lsd1 in wing development of drosophila melanogaster |
| topic | Drosophila lipid storage droplet 1 mitochondrial stress perilipin wing development |
| url | http://www.mdpi.com/1422-0067/17/5/648 |
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