Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells
<p>Abstract</p> <p>Background</p> <p>Neuropeptide Y (NPY) is emerging as a modulator of communication between the brain and the immune system. However, in spite of increasing evidence that supports a role for NPY in the modulation of microglial cell responses to inflamm...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-12-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | http://www.jneuroinflammation.com/content/8/1/169 |
| _version_ | 1818807415124000768 |
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| author | Ferreira Raquel Santos Tiago Viegas Michelle Cortes Luísa Bernardino Liliana Vieira Otília V Malva João O |
| author_facet | Ferreira Raquel Santos Tiago Viegas Michelle Cortes Luísa Bernardino Liliana Vieira Otília V Malva João O |
| author_sort | Ferreira Raquel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Neuropeptide Y (NPY) is emerging as a modulator of communication between the brain and the immune system. However, in spite of increasing evidence that supports a role for NPY in the modulation of microglial cell responses to inflammatory conditions, there is no consistent information regarding the action of NPY on microglial phagocytic activity, a vital component of the inflammatory response in brain injury. Taking this into consideration, we sought to assess a potential new role for NPY as a modulator of phagocytosis by microglial cells.</p> <p>Methods</p> <p>The N9 murine microglial cell line was used to evaluate the role of NPY in phagocytosis. For that purpose, an IgG-opsonized latex bead assay was performed in the presence of lipopolysaccharide (LPS) and an interleukin-1β (IL-1β) challenge, and upon NPY treatment. A pharmacological approach using NPY receptor agonists and antagonists followed to uncover which NPY receptor was involved. Moreover, western blotting and immunocytochemical studies were performed to evaluate expression of p38 mitogen-activated protein kinase (MAPK) and heat shock protein 27 (HSP27), in an inflammatory context, upon NPY treatment.</p> <p>Results</p> <p>Here, we show that NPY inhibits phagocytosis of opsonized latex beads and inhibits actin cytoskeleton reorganization triggered by LPS stimulation. Co-stimulation of microglia with LPS and adenosine triphosphate also resulted in increased phagocytosis, an effect inhibited by an interleukin-1 receptor antagonist, suggesting involvement of IL-1β signaling. Furthermore, direct application of LPS or IL-1β activated downstream signaling molecules, including p38 MAPK and HSP27, and these effects were inhibited by NPY. Moreover, we also observed that the inhibitory effect of NPY on phagocytosis was mediated <it>via </it>Y<sub>1 </sub>receptor activation.</p> <p>Conclusions</p> <p>Altogether, we have identified a novel role for NPY in the regulation of microglial phagocytic properties, in an inflammatory context.</p> |
| first_indexed | 2024-12-18T19:25:18Z |
| format | Article |
| id | doaj.art-f38cb916b3c5446ca45467579c2e263c |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-18T19:25:18Z |
| publishDate | 2011-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-f38cb916b3c5446ca45467579c2e263c2022-12-21T20:55:54ZengBMCJournal of Neuroinflammation1742-20942011-12-018116910.1186/1742-2094-8-169Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cellsFerreira RaquelSantos TiagoViegas MichelleCortes LuísaBernardino LilianaVieira Otília VMalva João O<p>Abstract</p> <p>Background</p> <p>Neuropeptide Y (NPY) is emerging as a modulator of communication between the brain and the immune system. However, in spite of increasing evidence that supports a role for NPY in the modulation of microglial cell responses to inflammatory conditions, there is no consistent information regarding the action of NPY on microglial phagocytic activity, a vital component of the inflammatory response in brain injury. Taking this into consideration, we sought to assess a potential new role for NPY as a modulator of phagocytosis by microglial cells.</p> <p>Methods</p> <p>The N9 murine microglial cell line was used to evaluate the role of NPY in phagocytosis. For that purpose, an IgG-opsonized latex bead assay was performed in the presence of lipopolysaccharide (LPS) and an interleukin-1β (IL-1β) challenge, and upon NPY treatment. A pharmacological approach using NPY receptor agonists and antagonists followed to uncover which NPY receptor was involved. Moreover, western blotting and immunocytochemical studies were performed to evaluate expression of p38 mitogen-activated protein kinase (MAPK) and heat shock protein 27 (HSP27), in an inflammatory context, upon NPY treatment.</p> <p>Results</p> <p>Here, we show that NPY inhibits phagocytosis of opsonized latex beads and inhibits actin cytoskeleton reorganization triggered by LPS stimulation. Co-stimulation of microglia with LPS and adenosine triphosphate also resulted in increased phagocytosis, an effect inhibited by an interleukin-1 receptor antagonist, suggesting involvement of IL-1β signaling. Furthermore, direct application of LPS or IL-1β activated downstream signaling molecules, including p38 MAPK and HSP27, and these effects were inhibited by NPY. Moreover, we also observed that the inhibitory effect of NPY on phagocytosis was mediated <it>via </it>Y<sub>1 </sub>receptor activation.</p> <p>Conclusions</p> <p>Altogether, we have identified a novel role for NPY in the regulation of microglial phagocytic properties, in an inflammatory context.</p>http://www.jneuroinflammation.com/content/8/1/169microglianeuropeptide YHSP27p38inflammationphagocytic cups |
| spellingShingle | Ferreira Raquel Santos Tiago Viegas Michelle Cortes Luísa Bernardino Liliana Vieira Otília V Malva João O Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells Journal of Neuroinflammation microglia neuropeptide Y HSP27 p38 inflammation phagocytic cups |
| title | Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells |
| title_full | Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells |
| title_fullStr | Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells |
| title_full_unstemmed | Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells |
| title_short | Neuropeptide Y inhibits interleukin-1β-induced phagocytosis by microglial cells |
| title_sort | neuropeptide y inhibits interleukin 1β induced phagocytosis by microglial cells |
| topic | microglia neuropeptide Y HSP27 p38 inflammation phagocytic cups |
| url | http://www.jneuroinflammation.com/content/8/1/169 |
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