Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic
ABSTRACT: Objectives: The aim of the study is to characterise the genomic features of three GES-producing Enterobacterales isolates from Czech hospitals. Methods: In 2020, during a routine screening of the hospital's surfaces in Prague General Hospital, two strains (CZ862 and CZ863) that belon...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522000509 |
| _version_ | 1828815257416499200 |
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| author | Marc Finianos Lucie Kraftova Costas C. Papagiannitsis Vaclava Adamkova Jaroslav Hrabak Ibrahim Bitar |
| author_facet | Marc Finianos Lucie Kraftova Costas C. Papagiannitsis Vaclava Adamkova Jaroslav Hrabak Ibrahim Bitar |
| author_sort | Marc Finianos |
| collection | DOAJ |
| description | ABSTRACT: Objectives: The aim of the study is to characterise the genomic features of three GES-producing Enterobacterales isolates from Czech hospitals. Methods: In 2020, during a routine screening of the hospital's surfaces in Prague General Hospital, two strains (CZ862 and CZ863) that belonged to the Enterobacter cloacae complex were found to be blaGES positive. Another blaGES positive strain identified as Klebsiella oxytoca was recovered from a patient hospitalised in Pilsen. Antibiotic susceptibility profiling was done with broth microdilution assay. Conjugation/transformation experiments were performed on all three strains. Genomic DNA of the three isolates was subjected to whole genome sequencing using PacBio platform. Results: Multilocus sequence types typing of CZ862 and CZ863 identified the strains as ST837 and a novel ST (ST1622). Both blaGES harbouring plasmids showed high sequence similarity and complete query coverage (100% and 99.98%) with pEcl-35771cz. Both plasmids had two copies of blaGES instead of one copy as found in pEcl-35771cz. The clinical isolate CZ598 belonged to ST180. The plasmid harboured blaGES-7 gene, cat and aac(6’)-lb and the novel variant blaOXA-1011. No similar sequences were observed, suggesting a novel plasmid. Conclusion: The detection of the two blaGES-positive plasmids in the same hospital environment, the first report after 3 years, suggests a hidden source. This highlights the importance of the hidden sources and evolution of such plasmids on the route of spreading into clinical settings. Also, the detection of the new blaOXA-1011, which is thought in this case to be associated with carbapenem resistance, imposes a health risk if disseminated, limiting therapeutic options. |
| first_indexed | 2024-12-12T10:41:06Z |
| format | Article |
| id | doaj.art-f38ccd91713743fc800ad64909a5ba71 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-12T10:41:06Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-f38ccd91713743fc800ad64909a5ba712022-12-22T00:27:04ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-06-0129116119Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech RepublicMarc Finianos0Lucie Kraftova1Costas C. Papagiannitsis2Vaclava Adamkova3Jaroslav Hrabak4Ibrahim Bitar5Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech RepublicDepartment of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech RepublicDepartment of Microbiology, University Hospital Larissa, GreeceDepartment of Clinical Microbiology, General University Hospital, Prague, Czech RepublicDepartment of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech RepublicDepartment of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic; Corresponding author. Mailing address: Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Alej Svobody 80, 304 60 Plzen, Czech Republic.ABSTRACT: Objectives: The aim of the study is to characterise the genomic features of three GES-producing Enterobacterales isolates from Czech hospitals. Methods: In 2020, during a routine screening of the hospital's surfaces in Prague General Hospital, two strains (CZ862 and CZ863) that belonged to the Enterobacter cloacae complex were found to be blaGES positive. Another blaGES positive strain identified as Klebsiella oxytoca was recovered from a patient hospitalised in Pilsen. Antibiotic susceptibility profiling was done with broth microdilution assay. Conjugation/transformation experiments were performed on all three strains. Genomic DNA of the three isolates was subjected to whole genome sequencing using PacBio platform. Results: Multilocus sequence types typing of CZ862 and CZ863 identified the strains as ST837 and a novel ST (ST1622). Both blaGES harbouring plasmids showed high sequence similarity and complete query coverage (100% and 99.98%) with pEcl-35771cz. Both plasmids had two copies of blaGES instead of one copy as found in pEcl-35771cz. The clinical isolate CZ598 belonged to ST180. The plasmid harboured blaGES-7 gene, cat and aac(6’)-lb and the novel variant blaOXA-1011. No similar sequences were observed, suggesting a novel plasmid. Conclusion: The detection of the two blaGES-positive plasmids in the same hospital environment, the first report after 3 years, suggests a hidden source. This highlights the importance of the hidden sources and evolution of such plasmids on the route of spreading into clinical settings. Also, the detection of the new blaOXA-1011, which is thought in this case to be associated with carbapenem resistance, imposes a health risk if disseminated, limiting therapeutic options.http://www.sciencedirect.com/science/article/pii/S2213716522000509blaGES-1blaGES-5blaGES-7blaOXA-1011Enterobacter cloacaeKlebsiella michiganensis |
| spellingShingle | Marc Finianos Lucie Kraftova Costas C. Papagiannitsis Vaclava Adamkova Jaroslav Hrabak Ibrahim Bitar Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic Journal of Global Antimicrobial Resistance blaGES-1 blaGES-5 blaGES-7 blaOXA-1011 Enterobacter cloacae Klebsiella michiganensis |
| title | Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic |
| title_full | Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic |
| title_fullStr | Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic |
| title_full_unstemmed | Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic |
| title_short | Genomic characterisation of three GES-producing Enterobacterales isolated in the Czech Republic |
| title_sort | genomic characterisation of three ges producing enterobacterales isolated in the czech republic |
| topic | blaGES-1 blaGES-5 blaGES-7 blaOXA-1011 Enterobacter cloacae Klebsiella michiganensis |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522000509 |
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