Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China
Aquatic environments are important reservoirs for drug resistance. Aquatic foods may act as carriers to lead antibiotic-resistant commensal bacteria into the human gastrointestinal system, then contacting gut microbiota and spreading antibiotic resistance. Here, several shrimp farms were investigate...
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MDPI AG
2023-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/11/2143 |
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| author | Yilin Zhang Xinrui Lv Weiwei Cao Huang Zhang Lei Shi Weibin Bai Lei Ye |
| author_facet | Yilin Zhang Xinrui Lv Weiwei Cao Huang Zhang Lei Shi Weibin Bai Lei Ye |
| author_sort | Yilin Zhang |
| collection | DOAJ |
| description | Aquatic environments are important reservoirs for drug resistance. Aquatic foods may act as carriers to lead antibiotic-resistant commensal bacteria into the human gastrointestinal system, then contacting gut microbiota and spreading antibiotic resistance. Here, several shrimp farms were investigated to identify colistin resistance among commensal bacteria of aquaculture. A total of 884 (41.6%) colistin-resistant isolates were identified among 2126 strains. Electroporation demonstrated that colistin-resistant fragments were present in some commensal bacteria that could be transferred to other bacteria. Most of the resistant bacteria were <i>Bacillus</i> spp., with 69.3% of the <i>Bacillus</i> species exhibiting multiple drug resistance. <i>Bacillus licheniformis</i> was prevalent, with 58 strains identified that comprised six sequence types (ST) based on multilocus sequence typing. Whole-genome sequencing and comparisons with previous <i>B. licheniformis</i> genomes revealed a high degree of genomic similarity among isolates from different regions. Thus, this species is widely distributed, and this study provides new insights into global antibiotic-resistant characteristics of <i>B. licheniformis</i>. Sequence analyses further revealed some of these strains are even pathogenic and virulent, suggesting the antibiotic resistance and hazards of commensal bacteria in aquaculture should be considered. Considering the “One Health” perspective, improved monitoring of aquatic food is needed to prevent the spread of drug-resistant commensal bacteria from food-associated bacteria to humans. |
| first_indexed | 2024-03-11T03:08:08Z |
| format | Article |
| id | doaj.art-f38f2c8e344f4f7681f22f524a21eb14 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T03:08:08Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-f38f2c8e344f4f7681f22f524a21eb142023-11-18T07:50:51ZengMDPI AGFoods2304-81582023-05-011211214310.3390/foods12112143Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in ChinaYilin Zhang0Xinrui Lv1Weiwei Cao2Huang Zhang3Lei Shi4Weibin Bai5Lei Ye6Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, ChinaInstitute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, ChinaSchool of Food Science and Biology, Guangdong Polytechnic of Science and Trade, Guangzhou 510640, ChinaGuangzhou Double Helix Gene Technology Co., Ltd., Guangzhou International Bio Island Co., Ltd., Guangzhou 510320, ChinaInstitute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, ChinaInstitute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, ChinaInstitute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, ChinaAquatic environments are important reservoirs for drug resistance. Aquatic foods may act as carriers to lead antibiotic-resistant commensal bacteria into the human gastrointestinal system, then contacting gut microbiota and spreading antibiotic resistance. Here, several shrimp farms were investigated to identify colistin resistance among commensal bacteria of aquaculture. A total of 884 (41.6%) colistin-resistant isolates were identified among 2126 strains. Electroporation demonstrated that colistin-resistant fragments were present in some commensal bacteria that could be transferred to other bacteria. Most of the resistant bacteria were <i>Bacillus</i> spp., with 69.3% of the <i>Bacillus</i> species exhibiting multiple drug resistance. <i>Bacillus licheniformis</i> was prevalent, with 58 strains identified that comprised six sequence types (ST) based on multilocus sequence typing. Whole-genome sequencing and comparisons with previous <i>B. licheniformis</i> genomes revealed a high degree of genomic similarity among isolates from different regions. Thus, this species is widely distributed, and this study provides new insights into global antibiotic-resistant characteristics of <i>B. licheniformis</i>. Sequence analyses further revealed some of these strains are even pathogenic and virulent, suggesting the antibiotic resistance and hazards of commensal bacteria in aquaculture should be considered. Considering the “One Health” perspective, improved monitoring of aquatic food is needed to prevent the spread of drug-resistant commensal bacteria from food-associated bacteria to humans.https://www.mdpi.com/2304-8158/12/11/2143antibiotic resistanceaquatic shrimpprevalencewhole-genome sequencinggenome-wide association study |
| spellingShingle | Yilin Zhang Xinrui Lv Weiwei Cao Huang Zhang Lei Shi Weibin Bai Lei Ye Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China Foods antibiotic resistance aquatic shrimp prevalence whole-genome sequencing genome-wide association study |
| title | Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China |
| title_full | Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China |
| title_fullStr | Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China |
| title_full_unstemmed | Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China |
| title_short | Survey of Colistin Resistance in Commensal Bacteria from <i>Penaeus vannamei</i> Farms in China |
| title_sort | survey of colistin resistance in commensal bacteria from i penaeus vannamei i farms in china |
| topic | antibiotic resistance aquatic shrimp prevalence whole-genome sequencing genome-wide association study |
| url | https://www.mdpi.com/2304-8158/12/11/2143 |
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