Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering
3D-printed polylactic acid (PLA) scaffolds have been demonstrated as being a promising tool for the development of tissue-engineered replacements of bone. However, this material lacks a suitable surface chemistry to efficiently interact with extracellular proteins and, consequently, to integrate int...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/7/2576 |
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| author | Ricardo Donate María Elena Alemán-Domínguez Mario Monzón Jianshu Yu Francisco Rodríguez-Esparragón Chaozong Liu |
| author_facet | Ricardo Donate María Elena Alemán-Domínguez Mario Monzón Jianshu Yu Francisco Rodríguez-Esparragón Chaozong Liu |
| author_sort | Ricardo Donate |
| collection | DOAJ |
| description | 3D-printed polylactic acid (PLA) scaffolds have been demonstrated as being a promising tool for the development of tissue-engineered replacements of bone. However, this material lacks a suitable surface chemistry to efficiently interact with extracellular proteins and, consequently, to integrate into the surrounding tissue when implanted in vivo. In this study, aloe vera coatings have been proposed as a strategy to improve the bioaffinity of this type of structures. Aloe vera coatings were applied at three different values of pH (3, 4 and 5), after treating the surface of the PLA scaffolds with oxygen plasma. The surface modification of the material has been assessed through X-ray photoelectron spectroscopy (XPS) analysis and water contact angle measurements. In addition, the evaluation of the enzymatic degradation of the structures showed that the pH of the aloe vera extracts used as coating influences the degradation rate of the PLA-based scaffolds. Finally, the cell metabolic activity of an in vitro culture of human fetal osteoblastic cells on the samples revealed an improvement of this parameter on aloe vera coated samples, especially for those treated at pH 3. Hence, these structures showed potential for being applied for bone tissue regeneration. |
| first_indexed | 2024-03-10T20:35:25Z |
| format | Article |
| id | doaj.art-f38fde77fa7248e88408c130e190adda |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:35:25Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-f38fde77fa7248e88408c130e190adda2023-11-19T21:05:39ZengMDPI AGApplied Sciences2076-34172020-04-01107257610.3390/app10072576Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue EngineeringRicardo Donate0María Elena Alemán-Domínguez1Mario Monzón2Jianshu Yu3Francisco Rodríguez-Esparragón4Chaozong Liu5Departamento de Ingeniería Mecánica, Grupo de Investigación en Fabricación Integrada y Avanzada, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017 Las Palmas, SpainDepartamento de Ingeniería Mecánica, Grupo de Investigación en Fabricación Integrada y Avanzada, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017 Las Palmas, SpainDepartamento de Ingeniería Mecánica, Grupo de Investigación en Fabricación Integrada y Avanzada, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017 Las Palmas, SpainInstitute of Orthopaedic & Musculoskeletal Science, University College London, Royal National Orthopaedic Hospital, Stanmore HA4 4LP, London, UKUnidad de Investigación, Hospital Universitario de Gran Canaria Doctor Negrín, Barranco de la Ballena s/n, 35010 Las Palmas, SpainInstitute of Orthopaedic & Musculoskeletal Science, University College London, Royal National Orthopaedic Hospital, Stanmore HA4 4LP, London, UK3D-printed polylactic acid (PLA) scaffolds have been demonstrated as being a promising tool for the development of tissue-engineered replacements of bone. However, this material lacks a suitable surface chemistry to efficiently interact with extracellular proteins and, consequently, to integrate into the surrounding tissue when implanted in vivo. In this study, aloe vera coatings have been proposed as a strategy to improve the bioaffinity of this type of structures. Aloe vera coatings were applied at three different values of pH (3, 4 and 5), after treating the surface of the PLA scaffolds with oxygen plasma. The surface modification of the material has been assessed through X-ray photoelectron spectroscopy (XPS) analysis and water contact angle measurements. In addition, the evaluation of the enzymatic degradation of the structures showed that the pH of the aloe vera extracts used as coating influences the degradation rate of the PLA-based scaffolds. Finally, the cell metabolic activity of an in vitro culture of human fetal osteoblastic cells on the samples revealed an improvement of this parameter on aloe vera coated samples, especially for those treated at pH 3. Hence, these structures showed potential for being applied for bone tissue regeneration.https://www.mdpi.com/2076-3417/10/7/2576regenerative medicineadditive manufacturingplasma treatmentcoating methodaloe vera extractsosteoblast cells |
| spellingShingle | Ricardo Donate María Elena Alemán-Domínguez Mario Monzón Jianshu Yu Francisco Rodríguez-Esparragón Chaozong Liu Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering Applied Sciences regenerative medicine additive manufacturing plasma treatment coating method aloe vera extracts osteoblast cells |
| title | Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering |
| title_full | Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering |
| title_fullStr | Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering |
| title_full_unstemmed | Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering |
| title_short | Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering |
| title_sort | evaluation of aloe vera coated polylactic acid scaffolds for bone tissue engineering |
| topic | regenerative medicine additive manufacturing plasma treatment coating method aloe vera extracts osteoblast cells |
| url | https://www.mdpi.com/2076-3417/10/7/2576 |
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