Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions
Sorghum has a significant amount of proteins, especially kafirin; however, limited information is available on evaluating its potential for peptide antioxidants. The objectives of this study were to: (1) investigate the effects of two key variables, enzyme-to-substrate ratio and reaction time on kaf...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-05-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/8/5/131 |
| _version_ | 1827840133735383040 |
|---|---|
| author | Shiwei Xu Yanting Shen Yonghui Li |
| author_facet | Shiwei Xu Yanting Shen Yonghui Li |
| author_sort | Shiwei Xu |
| collection | DOAJ |
| description | Sorghum has a significant amount of proteins, especially kafirin; however, limited information is available on evaluating its potential for peptide antioxidants. The objectives of this study were to: (1) investigate the effects of two key variables, enzyme-to-substrate ratio and reaction time on kafirin hydrolysis using Alcalase; (2) evaluate the antioxidant performances of the hydrolysates and fractions from membrane ultrafiltration and gel filtration; and (3) identify peptide sequences in the antioxidant fraction using MALDI-TOF/TOF MS. Kafirin hydrolysates prepared at enzyme-to-substrate ratio of 0.4 Au/g and 4 h had a good balance of antioxidant activity, yield, and economic efficiency. Medium-sized fraction of hydrolysates (5–10 kDa) from membrane filtration possessed the highest antioxidant activities among various fractions. The fraction also unveiled a good inhibition effect against lipid oxidation in emulsion and ground meat systems. Smaller-sized fraction (F3) collected through gel-filtration chromatography had significantly stronger antioxidant activities than other fractions, and 26 representative peptide sequences were identified in the fraction. |
| first_indexed | 2024-03-12T07:29:45Z |
| format | Article |
| id | doaj.art-f392d6c7fea845979d141b6fbba27c7f |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T07:29:45Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f392d6c7fea845979d141b6fbba27c7f2023-09-02T21:53:52ZengMDPI AGAntioxidants2076-39212019-05-018513110.3390/antiox8050131antiox8050131Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated FractionsShiwei Xu0Yanting Shen1Yonghui Li2Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USADepartment of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USADepartment of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USASorghum has a significant amount of proteins, especially kafirin; however, limited information is available on evaluating its potential for peptide antioxidants. The objectives of this study were to: (1) investigate the effects of two key variables, enzyme-to-substrate ratio and reaction time on kafirin hydrolysis using Alcalase; (2) evaluate the antioxidant performances of the hydrolysates and fractions from membrane ultrafiltration and gel filtration; and (3) identify peptide sequences in the antioxidant fraction using MALDI-TOF/TOF MS. Kafirin hydrolysates prepared at enzyme-to-substrate ratio of 0.4 Au/g and 4 h had a good balance of antioxidant activity, yield, and economic efficiency. Medium-sized fraction of hydrolysates (5–10 kDa) from membrane filtration possessed the highest antioxidant activities among various fractions. The fraction also unveiled a good inhibition effect against lipid oxidation in emulsion and ground meat systems. Smaller-sized fraction (F3) collected through gel-filtration chromatography had significantly stronger antioxidant activities than other fractions, and 26 representative peptide sequences were identified in the fraction.https://www.mdpi.com/2076-3921/8/5/131grain sorghumkafirinprotein hydrolysateantioxidantemulsionground meatpeptide sequence |
| spellingShingle | Shiwei Xu Yanting Shen Yonghui Li Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions Antioxidants grain sorghum kafirin protein hydrolysate antioxidant emulsion ground meat peptide sequence |
| title | Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions |
| title_full | Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions |
| title_fullStr | Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions |
| title_full_unstemmed | Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions |
| title_short | Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions |
| title_sort | antioxidant activities of sorghum kafirin alcalase hydrolysates and membrane gel filtrated fractions |
| topic | grain sorghum kafirin protein hydrolysate antioxidant emulsion ground meat peptide sequence |
| url | https://www.mdpi.com/2076-3921/8/5/131 |
| work_keys_str_mv | AT shiweixu antioxidantactivitiesofsorghumkafirinalcalasehydrolysatesandmembranegelfiltratedfractions AT yantingshen antioxidantactivitiesofsorghumkafirinalcalasehydrolysatesandmembranegelfiltratedfractions AT yonghuili antioxidantactivitiesofsorghumkafirinalcalasehydrolysatesandmembranegelfiltratedfractions |