Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection
Infection of Herpes simplex virus 1 (HSV-1) induces severe clinical disorders, such as herpes simplex encephalitis and keratitis. Acyclovir (ACV) is the current therapeutic drug against viral infection and ACV-resistant strains have gradually emerged, leading to the requirement for novel antiviral a...
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| Format: | Article |
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MDPI AG
2019-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/5/466 |
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| author | Feng Li Xiaowei Song Guifeng Su Yiliang Wang Zhaoyang Wang Jiaoyan Jia Shurong Qing Lianzhou Huang Yuan Wang Kai Zheng Yifei Wang |
| author_facet | Feng Li Xiaowei Song Guifeng Su Yiliang Wang Zhaoyang Wang Jiaoyan Jia Shurong Qing Lianzhou Huang Yuan Wang Kai Zheng Yifei Wang |
| author_sort | Feng Li |
| collection | DOAJ |
| description | Infection of Herpes simplex virus 1 (HSV-1) induces severe clinical disorders, such as herpes simplex encephalitis and keratitis. Acyclovir (ACV) is the current therapeutic drug against viral infection and ACV-resistant strains have gradually emerged, leading to the requirement for novel antiviral agents. In this study, we exhibited the antiviral activity of amentoflavone, a naturally occurring biflavonoid, toward HSV-1 and ACV-resistant strains. Amentoflavone significantly inhibited infection of HSV-1 (F strain), as well as several ACV-resistant strains including HSV-1/106, HSV-1/153 and HSV-1/Blue at high concentrations. Time-of-drug-addition assay further revealed that amentoflavone mainly impaired HSV-1 early infection. More detailed study demonstrated that amentoflavone affected cofilin-mediated F-actin reorganization and reduced the intracellular transportation of HSV-1 from the cell membrane to the nucleus. In addition, amentoflavone substantially decreased transcription of viral immediate early genes. Collectively, amentoflavone showed strong antiviral activity against HSV-1 and ACV-resistant strains, and amentoflavone could be a promising therapeutic candidate for HSV-1 pathogenesis. |
| first_indexed | 2024-12-13T04:37:10Z |
| format | Article |
| id | doaj.art-f39e2311ffeb4e3a8cdf622ccf2e462d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T04:37:10Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f39e2311ffeb4e3a8cdf622ccf2e462d2022-12-21T23:59:25ZengMDPI AGViruses1999-49152019-05-0111546610.3390/v11050466v11050466Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early InfectionFeng Li0Xiaowei Song1Guifeng Su2Yiliang Wang3Zhaoyang Wang4Jiaoyan Jia5Shurong Qing6Lianzhou Huang7Yuan Wang8Kai Zheng9Yifei Wang10Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaSchool of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, ChinaInstitute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInfection of Herpes simplex virus 1 (HSV-1) induces severe clinical disorders, such as herpes simplex encephalitis and keratitis. Acyclovir (ACV) is the current therapeutic drug against viral infection and ACV-resistant strains have gradually emerged, leading to the requirement for novel antiviral agents. In this study, we exhibited the antiviral activity of amentoflavone, a naturally occurring biflavonoid, toward HSV-1 and ACV-resistant strains. Amentoflavone significantly inhibited infection of HSV-1 (F strain), as well as several ACV-resistant strains including HSV-1/106, HSV-1/153 and HSV-1/Blue at high concentrations. Time-of-drug-addition assay further revealed that amentoflavone mainly impaired HSV-1 early infection. More detailed study demonstrated that amentoflavone affected cofilin-mediated F-actin reorganization and reduced the intracellular transportation of HSV-1 from the cell membrane to the nucleus. In addition, amentoflavone substantially decreased transcription of viral immediate early genes. Collectively, amentoflavone showed strong antiviral activity against HSV-1 and ACV-resistant strains, and amentoflavone could be a promising therapeutic candidate for HSV-1 pathogenesis.https://www.mdpi.com/1999-4915/11/5/466herpes simplex virus 1ACV-resistant strainsamentoflavonecofilinF-actin dynamic |
| spellingShingle | Feng Li Xiaowei Song Guifeng Su Yiliang Wang Zhaoyang Wang Jiaoyan Jia Shurong Qing Lianzhou Huang Yuan Wang Kai Zheng Yifei Wang Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection Viruses herpes simplex virus 1 ACV-resistant strains amentoflavone cofilin F-actin dynamic |
| title | Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection |
| title_full | Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection |
| title_fullStr | Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection |
| title_full_unstemmed | Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection |
| title_short | Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection |
| title_sort | amentoflavone inhibits hsv 1 and acv resistant strain infection by suppressing viral early infection |
| topic | herpes simplex virus 1 ACV-resistant strains amentoflavone cofilin F-actin dynamic |
| url | https://www.mdpi.com/1999-4915/11/5/466 |
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