Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer
During open heart surgery, the haemostasis status of a patient has to be monitored permanently in order to quickly and reliably detect bleeding or coagulation-based disorders. Currently, no single medical device is available to provide a comprehensive solution for monitoring the coagulation status (...
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MDPI AG
2018-01-01
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| Series: | Applied Sciences |
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| Online Access: | http://www.mdpi.com/2076-3417/8/1/84 |
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| author | Silju-John Kunnakattu Theresia Groß Juvano Knieps Tim Kemper Stefan Fennrich Nicole Rauch Hans P. Wendel Sandra Stoppelkamp |
| author_facet | Silju-John Kunnakattu Theresia Groß Juvano Knieps Tim Kemper Stefan Fennrich Nicole Rauch Hans P. Wendel Sandra Stoppelkamp |
| author_sort | Silju-John Kunnakattu |
| collection | DOAJ |
| description | During open heart surgery, the haemostasis status of a patient has to be monitored permanently in order to quickly and reliably detect bleeding or coagulation-based disorders. Currently, no single medical device is available to provide a comprehensive solution for monitoring the coagulation status (coagulation, platelets and fibrinolysis). We intend to approach this problem with a rheological method. Here, we compared the performance of an oscillatory rheometer with a medical reference device, a ball coagulometer. Measuring the extrinsic coagulation (prothrombin time; PT), various heparin concentrations (0.5–2.0 IU/mL) could be differentiated and also discriminated from the intrinsic coagulation (activated partial thromboplastin time; aPTT) providing comparable clotting times between rheometer and ball coagulometer. In addition, the oscillatory rheometer was capable to detect the antagonising of heparin with the equimolar concentration of protamine and also the titration of various protamine concentrations (0.5–3.0 IU/mL) to a fixed heparin concentration (2 IU/mL). The addition of increasing concentrations of heparin to citrated blood prolonged the clotting time (CT), changed the slope calculated by linear regression of the elastic and viscous shear moduli (i.e., information of the coagulation process) and changed the value of the shear moduli at the end of the measurement (300 s). These results indicate that the oscillatory rheometer is capable to dynamically measure the haemostasis status with different activators and various inhibitor concentrations. |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-11T03:13:30Z |
| publishDate | 2018-01-01 |
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| series | Applied Sciences |
| spelling | doaj.art-f39f74d0bae246658414f3a4810806982022-12-22T01:22:50ZengMDPI AGApplied Sciences2076-34172018-01-01818410.3390/app8010084app8010084Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory RheometerSilju-John Kunnakattu0Theresia Groß1Juvano Knieps2Tim Kemper3Stefan Fennrich4Nicole Rauch5Hans P. Wendel6Sandra Stoppelkamp7Department of Thoracic, Cardiac and Vascular Surgery, Clinical Research Laboratory, University Hospital Tuebingen, 72076 Tuebingen, GermanyDepartment of Micro- and Nanoanalytics, University of Applied Sciences Iserlohn, 58644 Iserlohn, GermanyDepartment of Micro- and Nanoanalytics, University of Applied Sciences Iserlohn, 58644 Iserlohn, GermanyDepartment of Micro- and Nanoanalytics, University of Applied Sciences Iserlohn, 58644 Iserlohn, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, Clinical Research Laboratory, University Hospital Tuebingen, 72076 Tuebingen, GermanyDepartment of Micro- and Nanoanalytics, University of Applied Sciences Iserlohn, 58644 Iserlohn, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, Clinical Research Laboratory, University Hospital Tuebingen, 72076 Tuebingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, Clinical Research Laboratory, University Hospital Tuebingen, 72076 Tuebingen, GermanyDuring open heart surgery, the haemostasis status of a patient has to be monitored permanently in order to quickly and reliably detect bleeding or coagulation-based disorders. Currently, no single medical device is available to provide a comprehensive solution for monitoring the coagulation status (coagulation, platelets and fibrinolysis). We intend to approach this problem with a rheological method. Here, we compared the performance of an oscillatory rheometer with a medical reference device, a ball coagulometer. Measuring the extrinsic coagulation (prothrombin time; PT), various heparin concentrations (0.5–2.0 IU/mL) could be differentiated and also discriminated from the intrinsic coagulation (activated partial thromboplastin time; aPTT) providing comparable clotting times between rheometer and ball coagulometer. In addition, the oscillatory rheometer was capable to detect the antagonising of heparin with the equimolar concentration of protamine and also the titration of various protamine concentrations (0.5–3.0 IU/mL) to a fixed heparin concentration (2 IU/mL). The addition of increasing concentrations of heparin to citrated blood prolonged the clotting time (CT), changed the slope calculated by linear regression of the elastic and viscous shear moduli (i.e., information of the coagulation process) and changed the value of the shear moduli at the end of the measurement (300 s). These results indicate that the oscillatory rheometer is capable to dynamically measure the haemostasis status with different activators and various inhibitor concentrations.http://www.mdpi.com/2076-3417/8/1/84oscillatory rheometryblood coagulationhaemorheologyviscoelasticpoint-of-care |
| spellingShingle | Silju-John Kunnakattu Theresia Groß Juvano Knieps Tim Kemper Stefan Fennrich Nicole Rauch Hans P. Wendel Sandra Stoppelkamp Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer Applied Sciences oscillatory rheometry blood coagulation haemorheology viscoelastic point-of-care |
| title | Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer |
| title_full | Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer |
| title_fullStr | Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer |
| title_full_unstemmed | Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer |
| title_short | Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer |
| title_sort | dynamic and quantitative assessment of blood coagulation status with an oscillatory rheometer |
| topic | oscillatory rheometry blood coagulation haemorheology viscoelastic point-of-care |
| url | http://www.mdpi.com/2076-3417/8/1/84 |
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