Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells.
Epiblast stem cells (EpiSCs) are primed pluripotent stem cells and can be derived from postimplantation mouse embryos. We now show that the absence of canonical Wnt/β-catenin signaling is essential for maintenance of the undifferentiated state in mouse EpiSCs and in the epiblast of mouse embryos. At...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3653965?pdf=render |
| _version_ | 1811264675372859392 |
|---|---|
| author | Tomoyuki Sumi Shinya Oki Keiko Kitajima Chikara Meno |
| author_facet | Tomoyuki Sumi Shinya Oki Keiko Kitajima Chikara Meno |
| author_sort | Tomoyuki Sumi |
| collection | DOAJ |
| description | Epiblast stem cells (EpiSCs) are primed pluripotent stem cells and can be derived from postimplantation mouse embryos. We now show that the absence of canonical Wnt/β-catenin signaling is essential for maintenance of the undifferentiated state in mouse EpiSCs and in the epiblast of mouse embryos. Attenuation of Wnt signaling with the small-molecule inhibitor XAV939 or deletion of the β-catenin gene blocked spontaneous differentiation of EpiSCs toward mesoderm and enhanced the expression of pluripotency factor genes, allowing propagation of EpiSCs as a homogeneous population. EpiSCs were efficiently established and propagated from single epiblast cells in the presence of both XAV939 and the Rho kinase (ROCK) inhibitor Y27632. Cell transplantation revealed that EpiSCs were able to contribute to primordial germ cells and descendants of all three germ layers in a host embryo, suggesting that they maintained pluripotency, even after prolonged culture with XAV939. Such an improvement in the homogeneity of pluripotency achieved with the use of a Wnt inhibitor should prove advantageous for manipulation of primed pluripotent stem cells. |
| first_indexed | 2024-04-12T20:08:37Z |
| format | Article |
| id | doaj.art-f3a47ac579164cb0ab112892f11d17e0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:08:37Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f3a47ac579164cb0ab112892f11d17e02022-12-22T03:18:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6337810.1371/journal.pone.0063378Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells.Tomoyuki SumiShinya OkiKeiko KitajimaChikara MenoEpiblast stem cells (EpiSCs) are primed pluripotent stem cells and can be derived from postimplantation mouse embryos. We now show that the absence of canonical Wnt/β-catenin signaling is essential for maintenance of the undifferentiated state in mouse EpiSCs and in the epiblast of mouse embryos. Attenuation of Wnt signaling with the small-molecule inhibitor XAV939 or deletion of the β-catenin gene blocked spontaneous differentiation of EpiSCs toward mesoderm and enhanced the expression of pluripotency factor genes, allowing propagation of EpiSCs as a homogeneous population. EpiSCs were efficiently established and propagated from single epiblast cells in the presence of both XAV939 and the Rho kinase (ROCK) inhibitor Y27632. Cell transplantation revealed that EpiSCs were able to contribute to primordial germ cells and descendants of all three germ layers in a host embryo, suggesting that they maintained pluripotency, even after prolonged culture with XAV939. Such an improvement in the homogeneity of pluripotency achieved with the use of a Wnt inhibitor should prove advantageous for manipulation of primed pluripotent stem cells.http://europepmc.org/articles/PMC3653965?pdf=render |
| spellingShingle | Tomoyuki Sumi Shinya Oki Keiko Kitajima Chikara Meno Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells. PLoS ONE |
| title | Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells. |
| title_full | Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells. |
| title_fullStr | Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells. |
| title_full_unstemmed | Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells. |
| title_short | Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells. |
| title_sort | epiblast ground state is controlled by canonical wnt β catenin signaling in the postimplantation mouse embryo and epiblast stem cells |
| url | http://europepmc.org/articles/PMC3653965?pdf=render |
| work_keys_str_mv | AT tomoyukisumi epiblastgroundstateiscontrolledbycanonicalwntbcateninsignalinginthepostimplantationmouseembryoandepiblaststemcells AT shinyaoki epiblastgroundstateiscontrolledbycanonicalwntbcateninsignalinginthepostimplantationmouseembryoandepiblaststemcells AT keikokitajima epiblastgroundstateiscontrolledbycanonicalwntbcateninsignalinginthepostimplantationmouseembryoandepiblaststemcells AT chikarameno epiblastgroundstateiscontrolledbycanonicalwntbcateninsignalinginthepostimplantationmouseembryoandepiblaststemcells |