Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the anti...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-07-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/8/7/735 |
| _version_ | 1797728520570404864 |
|---|---|
| author | Mária Čížková Monika Slavková Milada Vítová Vilém Zachleder Kateřina Bišová |
| author_facet | Mária Čížková Monika Slavková Milada Vítová Vilém Zachleder Kateřina Bišová |
| author_sort | Mária Čížková |
| collection | DOAJ |
| description | DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga <i>Chlamydomonas reinhardtii</i>. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in <i>C. reinhardtii</i> is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity. |
| first_indexed | 2024-03-12T11:15:14Z |
| format | Article |
| id | doaj.art-f3a6c38f4a0e442093b805bc98f4a82a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T11:15:14Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f3a6c38f4a0e442093b805bc98f4a82a2023-09-02T02:11:30ZengMDPI AGCells2073-44092019-07-018773510.3390/cells8070735cells8070735Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent ZeocinMária Čížková0Monika Slavková1Milada Vítová2Vilém Zachleder3Kateřina Bišová4Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czech RepublicLaboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czech RepublicLaboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czech RepublicLaboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czech RepublicLaboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czech RepublicDNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga <i>Chlamydomonas reinhardtii</i>. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in <i>C. reinhardtii</i> is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity.https://www.mdpi.com/2073-4409/8/7/735DNA damagedouble-stranded breakzeocincaffeinecell cyclecyclin-dependent kinase<i>Chlamydomonas reinhardtii</i> |
| spellingShingle | Mária Čížková Monika Slavková Milada Vítová Vilém Zachleder Kateřina Bišová Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin Cells DNA damage double-stranded break zeocin caffeine cell cycle cyclin-dependent kinase <i>Chlamydomonas reinhardtii</i> |
| title | Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin |
| title_full | Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin |
| title_fullStr | Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin |
| title_full_unstemmed | Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin |
| title_short | Response of the Green Alga <i>Chlamydomonas reinhardtii</i> to the DNA Damaging Agent Zeocin |
| title_sort | response of the green alga i chlamydomonas reinhardtii i to the dna damaging agent zeocin |
| topic | DNA damage double-stranded break zeocin caffeine cell cycle cyclin-dependent kinase <i>Chlamydomonas reinhardtii</i> |
| url | https://www.mdpi.com/2073-4409/8/7/735 |
| work_keys_str_mv | AT mariacizkova responseofthegreenalgaichlamydomonasreinhardtiiitothednadamagingagentzeocin AT monikaslavkova responseofthegreenalgaichlamydomonasreinhardtiiitothednadamagingagentzeocin AT miladavitova responseofthegreenalgaichlamydomonasreinhardtiiitothednadamagingagentzeocin AT vilemzachleder responseofthegreenalgaichlamydomonasreinhardtiiitothednadamagingagentzeocin AT katerinabisova responseofthegreenalgaichlamydomonasreinhardtiiitothednadamagingagentzeocin |