Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides
Abstract As a highly infectious epidemic in aquaculture, Pseudomonas plecoglossicida infection results in high mortality of teleosts and serious economic losses. Host–pathogen interactions shape the outcome of an infection, yet we still understand little about the molecular mechanism of these pathog...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17387-6 |
| _version_ | 1798038081600749568 |
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| author | Jun Zeng Zhiqiang Yang Yue Zhong Yingli Zheng Jingwen Hao Gang Luo Qingpi Yan |
| author_facet | Jun Zeng Zhiqiang Yang Yue Zhong Yingli Zheng Jingwen Hao Gang Luo Qingpi Yan |
| author_sort | Jun Zeng |
| collection | DOAJ |
| description | Abstract As a highly infectious epidemic in aquaculture, Pseudomonas plecoglossicida infection results in high mortality of teleosts and serious economic losses. Host–pathogen interactions shape the outcome of an infection, yet we still understand little about the molecular mechanism of these pathogen-mediated processes. Here, a P. plecoglossicida strain (NZBD9) and Epinephelus coioides were investigated as a model system to characterize pathogen-induced host metabolic remodeling over the course of infection. We present a non-targeted metabolomics profiling of E. coioides spleens from uninfected E. coioides and those infected with wild-type and clpV-RNA interference (RNAi) strains. The most significant changes of E. coioides upon infection were associated with amino acids, lysophospatidylcholines, and unsaturated fatty acids, involving disturbances in host nutritional utilization and immune responses. Dihydrosphingosine and fatty acid 16:2 were screened as potential biomarkers for assessing P. plecoglossicida infection. The silencing of the P. plecoglossicida clpV gene significantly recovered the lipid metabolism of infected E. coioides. This comprehensive metabolomics study provides novel insights into how P. plecoglossicida shape host metabolism to support their survival and replication and highlights the potential of the virulence gene clpV in the treatment of P. plecoglossicida infection in aquaculture. |
| first_indexed | 2024-04-11T21:35:21Z |
| format | Article |
| id | doaj.art-f3a79063418c40f59268ffe217f9b2ed |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T21:35:21Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f3a79063418c40f59268ffe217f9b2ed2022-12-22T04:01:47ZengNature PortfolioScientific Reports2045-23222022-08-0112111110.1038/s41598-022-17387-6Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioidesJun Zeng0Zhiqiang Yang1Yue Zhong2Yingli Zheng3Jingwen Hao4Gang Luo5Qingpi Yan6College of Ocean Food and Biological Engineering, Jimei UniversityCollege of Ocean Food and Biological Engineering, Jimei UniversityCollege of Ocean Food and Biological Engineering, Jimei UniversityCollege of Ocean Food and Biological Engineering, Jimei UniversityCollege of Ocean Food and Biological Engineering, Jimei UniversityFisheries College, Jimei UniversityFisheries College, Jimei UniversityAbstract As a highly infectious epidemic in aquaculture, Pseudomonas plecoglossicida infection results in high mortality of teleosts and serious economic losses. Host–pathogen interactions shape the outcome of an infection, yet we still understand little about the molecular mechanism of these pathogen-mediated processes. Here, a P. plecoglossicida strain (NZBD9) and Epinephelus coioides were investigated as a model system to characterize pathogen-induced host metabolic remodeling over the course of infection. We present a non-targeted metabolomics profiling of E. coioides spleens from uninfected E. coioides and those infected with wild-type and clpV-RNA interference (RNAi) strains. The most significant changes of E. coioides upon infection were associated with amino acids, lysophospatidylcholines, and unsaturated fatty acids, involving disturbances in host nutritional utilization and immune responses. Dihydrosphingosine and fatty acid 16:2 were screened as potential biomarkers for assessing P. plecoglossicida infection. The silencing of the P. plecoglossicida clpV gene significantly recovered the lipid metabolism of infected E. coioides. This comprehensive metabolomics study provides novel insights into how P. plecoglossicida shape host metabolism to support their survival and replication and highlights the potential of the virulence gene clpV in the treatment of P. plecoglossicida infection in aquaculture.https://doi.org/10.1038/s41598-022-17387-6 |
| spellingShingle | Jun Zeng Zhiqiang Yang Yue Zhong Yingli Zheng Jingwen Hao Gang Luo Qingpi Yan Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides Scientific Reports |
| title | Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides |
| title_full | Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides |
| title_fullStr | Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides |
| title_full_unstemmed | Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides |
| title_short | Metabolomics insights into the interaction between Pseudomonas plecoglossicida and Epinephelus coioides |
| title_sort | metabolomics insights into the interaction between pseudomonas plecoglossicida and epinephelus coioides |
| url | https://doi.org/10.1038/s41598-022-17387-6 |
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