Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals
Dislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates o...
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MDPI AG
2017-06-01
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| Series: | Crystals |
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| Online Access: | http://www.mdpi.com/2073-4352/7/6/170 |
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| author | Haruhiko Koizumi Satoshi Uda Kozo Fujiwara Junpei Okada Jun Nozawa |
| author_facet | Haruhiko Koizumi Satoshi Uda Kozo Fujiwara Junpei Okada Jun Nozawa |
| author_sort | Haruhiko Koizumi |
| collection | DOAJ |
| description | Dislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates of the crystal measured under an applied field at 1 MHz was observed, although the overall driving force increased. Assuming that the birth and spread mechanism of two-dimensional nucleation occurs, an increase in the effective surface energy of the step ends was realized in the presence of the electric field, which led to an improvement in the crystal quality of the tetragonal HEW lysozyme crystals. This article also discusses the increase in the effective surface energy of the step ends with respect to the change in the entropy of the solid. |
| first_indexed | 2024-04-11T13:38:10Z |
| format | Article |
| id | doaj.art-f3a799bce82c4253ab1f35819269684b |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-04-11T13:38:10Z |
| publishDate | 2017-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-f3a799bce82c4253ab1f35819269684b2022-12-22T04:21:23ZengMDPI AGCrystals2073-43522017-06-017617010.3390/cryst7060170cryst7060170Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme CrystalsHaruhiko Koizumi0Satoshi Uda1Kozo Fujiwara2Junpei Okada3Jun Nozawa4Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanDislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates of the crystal measured under an applied field at 1 MHz was observed, although the overall driving force increased. Assuming that the birth and spread mechanism of two-dimensional nucleation occurs, an increase in the effective surface energy of the step ends was realized in the presence of the electric field, which led to an improvement in the crystal quality of the tetragonal HEW lysozyme crystals. This article also discusses the increase in the effective surface energy of the step ends with respect to the change in the entropy of the solid.http://www.mdpi.com/2073-4352/7/6/170protein crystalsgrowth kineticselectric fieldcrystal quality |
| spellingShingle | Haruhiko Koizumi Satoshi Uda Kozo Fujiwara Junpei Okada Jun Nozawa Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals Crystals protein crystals growth kinetics electric field crystal quality |
| title | Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals |
| title_full | Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals |
| title_fullStr | Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals |
| title_full_unstemmed | Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals |
| title_short | Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals |
| title_sort | effect of an external electric field on the kinetics of dislocation free growth of tetragonal hen egg white lysozyme crystals |
| topic | protein crystals growth kinetics electric field crystal quality |
| url | http://www.mdpi.com/2073-4352/7/6/170 |
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