Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy
Pineapple is an economically important tropical fruit crop, but the lack of adequate planting material limits its productivity. A range of micropropagation protocols has been developed over the years to address this shortfall. Still, the final stage of micropropagation, i.e. acclimatisation, remains...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2020-10-01
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| Series: | The EuroBiotech Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/ebtj-2020-0026 |
| _version_ | 1818689564878831616 |
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| author | Soto Guillermo Lorente Gustavo Mendoza Jessica Báez Evelio Dany Lorenzo Carlos Manuel Rodríguez Romelio Hajari Elliosha Vicente Oscar Lorenzo José Carlos Baez Evelio Luis |
| author_facet | Soto Guillermo Lorente Gustavo Mendoza Jessica Báez Evelio Dany Lorenzo Carlos Manuel Rodríguez Romelio Hajari Elliosha Vicente Oscar Lorenzo José Carlos Baez Evelio Luis |
| author_sort | Soto Guillermo |
| collection | DOAJ |
| description | Pineapple is an economically important tropical fruit crop, but the lack of adequate planting material limits its productivity. A range of micropropagation protocols has been developed over the years to address this shortfall. Still, the final stage of micropropagation, i.e. acclimatisation, remains a challenge as pineapple plantlets grow very slowly. Several studies have been conducted focusing on this phase and attempting to improve plantlet growth and establishment, which requires tools for the non-destructive evaluation of growth during acclimatisation. This report describes the use of semi-automated and automated image analysis to quantify canopy growth of pineapple plantlets, during five months of acclimatisation. The canopy area progressively increased during acclimatisation, particularly after 90 days. Regression analyses were performed to determine the relationships between the automated image analysis and morphological indicators of growth. The mathematical relationships between estimations of the canopy area and the fresh and dry weights of intact plantlets, middle-aged leaves (D leaves) and roots showed determination coefficients (R2) between 0.84 and 0.92. We propose an appropriate tool for the simple, objective and non-destructive evaluation of pineapple plantlets growth, which can be generally applied for plant phenotyping, to reduce costs and develop streamlined pipelines for the assessment of plant growth. |
| first_indexed | 2024-12-17T12:12:07Z |
| format | Article |
| id | doaj.art-f3a805fa9e0347dcbceb41bca915c4a5 |
| institution | Directory Open Access Journal |
| issn | 2564-615X |
| language | English |
| last_indexed | 2024-12-17T12:12:07Z |
| publishDate | 2020-10-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj.art-f3a805fa9e0347dcbceb41bca915c4a52022-12-21T21:49:21ZengSciendoThe EuroBiotech Journal2564-615X2020-10-014422322910.2478/ebtj-2020-0026ebtj-2020-0026Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopySoto Guillermo0Lorente Gustavo1Mendoza Jessica2Báez Evelio Dany3Lorenzo Carlos Manuel4Rodríguez Romelio5Hajari Elliosha6Vicente Oscar7Lorenzo José Carlos8Baez Evelio Luis9Bioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaPlant Improvement; Agricultural Research Council-Tropical and Subtropical Crops; Private BagX11208, Nelspruit, 1200, South AfricaInstitute for the Conservation and Improvement of Valencian Agrodiversity (COMAV), Universitat Politècnica de València, 46022Valencia, SpainBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaBioplant Center, University of Ciego de Ávila, Ciego de Ávila, 69450, CubaPineapple is an economically important tropical fruit crop, but the lack of adequate planting material limits its productivity. A range of micropropagation protocols has been developed over the years to address this shortfall. Still, the final stage of micropropagation, i.e. acclimatisation, remains a challenge as pineapple plantlets grow very slowly. Several studies have been conducted focusing on this phase and attempting to improve plantlet growth and establishment, which requires tools for the non-destructive evaluation of growth during acclimatisation. This report describes the use of semi-automated and automated image analysis to quantify canopy growth of pineapple plantlets, during five months of acclimatisation. The canopy area progressively increased during acclimatisation, particularly after 90 days. Regression analyses were performed to determine the relationships between the automated image analysis and morphological indicators of growth. The mathematical relationships between estimations of the canopy area and the fresh and dry weights of intact plantlets, middle-aged leaves (D leaves) and roots showed determination coefficients (R2) between 0.84 and 0.92. We propose an appropriate tool for the simple, objective and non-destructive evaluation of pineapple plantlets growth, which can be generally applied for plant phenotyping, to reduce costs and develop streamlined pipelines for the assessment of plant growth.https://doi.org/10.2478/ebtj-2020-0026ananas comosus (l.) merrimage analysisacclimatisationlarge-scale propagationmicropropagation |
| spellingShingle | Soto Guillermo Lorente Gustavo Mendoza Jessica Báez Evelio Dany Lorenzo Carlos Manuel Rodríguez Romelio Hajari Elliosha Vicente Oscar Lorenzo José Carlos Baez Evelio Luis Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy The EuroBiotech Journal ananas comosus (l.) merr image analysis acclimatisation large-scale propagation micropropagation |
| title | Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy |
| title_full | Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy |
| title_fullStr | Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy |
| title_full_unstemmed | Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy |
| title_short | Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy |
| title_sort | growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy |
| topic | ananas comosus (l.) merr image analysis acclimatisation large-scale propagation micropropagation |
| url | https://doi.org/10.2478/ebtj-2020-0026 |
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